Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8‐hydroxy‐2′‐deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment

Abstract

Ratios of urinary 8-hydroxy-2'-deoxyguanosine to urinary creatinine (8-OHdG/creatinine) have been considered as a good biological indicator of DNA oxidation. Urinary 8-OHdG/ creatinine levels of lung cancer patients were evaluated by enzyme-linked iramunosorbent assay using a monoclonal antibody N45.1 during radiotherapy and chemotherapy. An increase in urinary 8-OHdG/creatinine was found in non-small-cell carcinoma (non-SCC) patients during the course of radiotherapy. SCC patients showed higher levels of urinary 8-OHdG/creatinine than the controls. Furthermore, SCC patients with complete or partial response to the chemotherapy showed a significant decrease in urinary 8-OHdG/creatinine while patients with no change or progressive disease showed an increase.

Effect of docosahexaenoic acid and eicosapentaenoic acid supplementation on oxidative stress levels during pregnancy

“…Nevertheless, MDA excretion seems to be a reliable indicator for LCPUFA peroxidation, as there were no other differences in dietary intake according to the dietary protocols (data not shown). Measurement of urinary 8-OHdG has become a well accepted marker of oxidative DNA damage in the human body [15] and it has been measured by several methods such as gas chromatography with mass spectrometric detection [41] and HPLC with electrochemical detection (ECD) [20]. Recently, an ELISA based on monoclonal IgG (N45.1 clone) was developed for estimation of 8-OHdG in urine [15], which made urinary 8-OHdG measurement much easier.…”

“…Measurement of urinary 8-OHdG has become a well accepted marker of oxidative DNA damage in the human body [15] and it has been measured by several methods such as gas chromatography with mass spectrometric detection [41] and HPLC with electrochemical detection (ECD) [20]. Recently, an ELISA based on monoclonal IgG (N45.1 clone) was developed for estimation of 8-OHdG in urine [15], which made urinary 8-OHdG measurement much easier. Yoshida et al [42] found a good correlation (r ¼ 0.88) between urinary 8-OHdG levels obtained with using ELISA (same kit as in our study) and HPLC-ECD.…”

“…8-Hydroxy-2 0 -deoxyguanosine (8-OHdG) is produced by oxidation of the nucleoside deoxyguanosine and is subsequently excreted directly into urine. Urinary 8-OHdG excretion has been identified as a sensitive marker for oxidative DNA damage [15]. Excessive oxidative stress was suggested to be causally involved in the development of pre-eclampsia and fetal growth retardation [16 -18], but there is only limited information on the potential effects of DHA supplementation on oxidative stress in pregnant women.…”

Determination of urinary oxidative DNA damage marker 8-hydroxy-2′-deoxyguanosine and the association with cigarette smoking

“…It's reported that within 8OHdG viral genomes introduce G:C → T:A transitions in vivo [10,11]. Due to its mutagenic potential, 8OHdG has been widely suggested as a biomarker for various cancers [12][13][14]. The concentration of 8OHdG is also evaluated in smokers and non-smokers [9,15,16] since cigarette smoke contains a large amount of oxygen radical forming substances, such as catechol and hydroquinone that may enhance lung carcinogenesis by free radical-mediated reactions.…”

Determination of 8-oxoguanine and 8-hydroxy-2′-deoxyguanosine in the rat cerebral cortex using microdialysis sampling and capillary electrophoresis with electrochemical detection

“…Reports of analytical methodologies for 8OHdG determination are more common than for 8oxoG, with many reports of 8oxoG being determined as 8OHdG. Elevated levels of 8OHdG have been correlated with exposure to ionizing radiation [8], industrial chemicals [9], air pollution [10], cigarette smoking [11], cancer [12,13], chemotherapy [14], and ischemia-reperfusion [15][16][17]. Although only a few are mentioned here, there are several hundred reports linking increased concentrations of 8OHdG to increased oxidative stress or disease states, with over twenty reports using 8OHdG as a biomarker for ischemia-reperfusion.…”