Biomarkers of Aspergillus spores: Strain typing and protein identification

Panel of monoclonal antibodies to sperm surface proteins as a tool for monitoring localization and identification of sperm–zona pellucida receptors

“…The protein spots destaining, cysteine residue modification, proteolytic digestion, peptide extraction and sample preparation for mass spectrometry was performed as described previously (Sulc et al 2009). The protein digestion was carried out in a cleavage buffer containing 0.05 M 4-ethylmorpholine acetate, 10 % (v/v) acetonitrile and sequencing grade trypsine endoprotease (Promega, 50 ng/μl) overnight at 37°C.…”

Intact spore MALDI-TOF mass spectrometry and proteomic analysis of Puccinia pathogenic fungi

“…Moreover, because of the hydrophobicity of spores, it was difficult to add the second matrix layer. In a MALDI-based study on Aspergillus fungi, the authors previously optimized a similar amount of 50 μg of spores per spot with a limit of detection at 0.31 μg [17].…”

“…After a great success, which had accompanied MALDI-based bacterial identification since its introduction [10], numerous protocols were optimized for microscopic fungi [11,12]. The procedure of sample preparation including the choice of matrix, solvents and sample deposition technique (namely with respect to the homogeneity of sample-matrix co-crystals) is the most critical part [12][13][14][15][16][17]. MALDI-TOF mass spectra are acquired in the m/z range of 2000 to 20,000 and searched against a database of characteristic protein profiles [18].…”

Capture of Methane by Fungi: Evidence from Laboratory-Scale Biofilter and Chromatographic Isotherm Studies

“…Ultrapure sand (40 to 100 mesh, muffle furnaced to remove any residual organics) was used as inert control media, while fungal materials were homogenized with sand in various ratios for the treatments. Fungal spores were used for their uniformity and, like fungal hyphae, are known to have hydrophobic surface properties (Sulc et al, 2009;Tucker and Talbot, 2001). Spores representing both major fungal phyla (Basidiomycota and Ascomycota) were tested.…”

“…The use of inactive resting spores supports the notion that this is not an active biodegradation process but rather a biosorption process. Like hyphae, spores are known to possess hydrophobic protein sheaths (Sulc et al, 2009;Tucker and Talbot, 2001). Used by spores for attachment to plant hosts, these hydrophobin layers also appear capable of capturing a poorly soluble GHG.…”