Biomedical and Medical Applications of Nitroxides

Likhtenshtein, Gertz I.; Yamauchi, Jun; Nakatsuji, Shin’ichi; Smirnov, Alex I.; Tamura, Rui

doi:10.1002/9783527621743.ch11

Cited by 6 publications

(2 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nitroxides are long-lived, stable, free radical species that contain a disubstituted nitrogen atom linked to a univalent oxygen atom (19). Nitroxides are considered sterically hindered, structural mimics of NO because both types of compounds contain an unpaired electron, which is delocalized over the nitrogen-oxygen bond.…”

mentioning

confidence: 99%

Nitroxide Functionalized Antibiotics Are Promising Eradication Agents against Staphylococcus aureus Biofilms

Verderosa

Dhouib

Fairfull‐Smith

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Treatment of biofilm-related Staphylococcus aureus infections represents an important medical challenge worldwide, as biofilms, even those involving drugsusceptible S. aureus strains, are highly refractory to conventional antibiotic therapy. Nitroxides were recently shown to induce the dispersal of Gram-negative biofilms in vitro, but their action against Gram-positive bacterial biofilms remains unknown.Here, we demonstrate that the biofilm dispersal activity of nitroxides extends to S. aureus, a clinically important Gram-positive pathogen. Coadministration of the nitroxide CTEMPO (4-carboxy-2,2,6,6-tetramethylpiperidin-1-yloxyl) with ciprofloxacin significantly improved the biofilm eradication activity of the antibiotic against S. aureus. Moreover, covalently linking the nitroxide to the antibiotic moiety further reduced the ciprofloxacin minimal biofilm eradication concentration. Microscopy analysis revealed that fluorescent nitroxide-antibiotic hybrids could penetrate S. aureus biofilms and enter cells localized at the surface and base of the biofilm structure. No toxicity to human cells was observed for the nitroxide CTEMPO or the nitroxide-antibiotic hybrids. Taken together, our results show that nitroxides can mediate the dispersal of Gram-positive biofilms and that dual-acting biofilm eradication antibiotics may provide broad-spectrum therapies for the treatment of biofilm-related infections.

show abstract

mentioning

confidence: 99%

Nitroxide Functionalized Antibiotics Are Promising Eradication Agents against Staphylococcus aureus Biofilms

Verderosa

Dhouib

Fairfull‐Smith

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Nitroxides are long-lived, stable free radical species, which contain a disubstituted nitrogen atom linked to a univalent oxygen atom (19). Nitroxides are considered sterically hindered, structural mimics of NO, as both compounds contain an unpaired electron, which is delocalized over the nitrogen-oxygen bond.…”

Section: Introductionmentioning

confidence: 99%

Nitroxide functionalized antibiotics are promising eradication agents against Staphylococcus aureus biofilms

Verderosa

Dhouib

Fairfull‐Smith

et al. 2019

Preprint

View full text Add to dashboard Cite

24 Treatment of Staphylococcus aureus biofilm-related infections represents an important 25 medical challenge worldwide, as biofilms, even of drug-susceptible S. aureus strains, are 26 highly refectory to conventional antibiotic therapy. Nitroxides were recently shown to induce 27 dispersal of Gram-negative biofilms in vitro, but their action against Gram-positive bacterial 28 biofilms remains unknown. Here we demonstrate that the biofilm dispersal activity of 29 nitroxides extends to S. aureus, a clinically important Gram-positive pathogen. Co-30 administration of the nitroxide CTEMPO with ciprofloxacin significantly improved the 31 antibiotic's biofilm-eradication activity against S. aureus. Moreover, covalently linking the 32 nitroxide to the antibiotic moiety further reduced ciprofloxacin's minimal biofilm eradication 33 concentration. Microscopy analysis revealed that fluorescent nitroxide-antibiotic hybrids 34 could penetrate S. aureus biofilms and enter into cells localising at the surface and base of 35 the biofilm structure. No toxicity was observed for the nitroxide CTEMPO and the nitroxide-36 antibiotic hybrids against human cells. Taken together, our results show that nitroxides can 37 mediate dispersal of Gram-positive biofilms and that dual-acting biofilm-eradication 38 antibiotics could provide broad-spectrum therapies for the treatment of biofilm-related 39 infections.40

show abstract

Long‐Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

et al. 2011

View full text Add to dashboard Cite

Vermessen von Nukleinsäure‐Strukturen: Gepulste Elektron‐Elektron‐Doppelresonanz‐Spektroskopie wurde zum ersten Mal verwendet, um die Struktur von Nukleinsäuren in Oozyten von Xenopus laevis zu vermessen (siehe Bild). Die in vitro und in Zellen gemessenen Abstände sind gleich, was auf die Existenz von stabilen Gesamtkonformationen der untersuchten haarnadelförmigen RNA und des Neomycin erkennenden RNA‐Schalters hindeutet.

show abstract

Biomedical and Medical Applications of Nitroxides

Cited by 6 publications

References 111 publications

Nitroxide Functionalized Antibiotics Are Promising Eradication Agents against Staphylococcus aureus Biofilms

Nitroxide Functionalized Antibiotics Are Promising Eradication Agents against Staphylococcus aureus Biofilms

Nitroxide functionalized antibiotics are promising eradication agents against Staphylococcus aureus biofilms

Long‐Range Distance Measurements on Nucleic Acids in Cells by Pulsed EPR Spectroscopy

Contact Info

Product

Resources

About