Biomedical properties and preparation of iron oxide-dextran nanostructures by MAPLE technique

Ciobanu, Carmen Steluţa; Iconaru, Simona Liliana; György, E.; Radu, Mihaela; Costache, Marieta; Dinischiotu, Anca; Coustumer, Philippe Le; Lafdi, Khalid; Predoi, Daniela

doi:10.1186/1752-153x-6-17

Cited by 45 publications

(42 citation statements)

References 50 publications

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“…11,17,19,28,36 However, the use of magnetic nanoparticles in CaP scaffolds for bone tissue engineering applications is rarely reported. In the current work, Fe 2 O 3 nanoparticles with the size of <50 nm were added to TCP precursors to achieve the Fe-doped TCP scaffolds.…”

Section: 0 Introductionmentioning

confidence: 99%

Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate

Vahabzadeh

Bose

2016

Ann Biomed Eng

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Iron (Fe) is a vital element and its deficiency causes abnormal bone metabolism. We investigated the effects of Fe and its concentration in β-tricalcium phosphate (β-TCP) on physicomechanical properties and in vitro proliferation and differentiation of osteoblasts. Our results showed that Fe addition at concentrations of 0.5 wt. % (0.5 Fe-TCP) and 1.0 wt. % (1.0 Fe-TCP) inhibits the β-TCP to α-TCP phase transformation at sintering temperature of 1250 °C. Addition of 0.25 wt. % Fe (0.25 Fe-TCP) increased the compressive strength of β-TCP from 167.27±16.2 MPa to 227.10±19.3 MPa. After 3 days of culture, surfaces of 0.5 Fe-TCP and 1.0 Fe-TCP samples were covered by osteoblast cells, compared to that of pure and 0.25 Fe-TCP. Cells grew to confluency on all Fe-doped samples after 7 days of culture and monolayer sheetlike cellular structure was found at 11 days. Optical cell density and alkaline phosphatase activity were significantly higher on Fe-doped samples and the highest values were found in 0.5 Fe-TCP samples. Our results show that Fe concentration had significant effect on physical and mechanical properties of TCP ceramics, and also on the in vitro osteoblast cellular interactions in TCP ceramics.

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Section: 0 Introductionmentioning

confidence: 99%

Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate

Vahabzadeh

Bose

2016

Ann Biomed Eng

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“…Nowadays, materials science has reached a stage of development that makes it possible to synthesize new engineered particles at a nanometric scale and to control their properties. Thus, tailoring the properties of nanoparticles for suitable biomedical, environmental, and industrial applications is one of the most studied areas of research worldwide [1][2][3]. Ideal candidates for developing nano-engineered structures with improved properties by functionalization are inorganic materials.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Balaș

Ciobanu

Burtéa

et al. 2017

Metals

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Abstract:We report the synthesis of dextran-coated iron oxide magnetic nanoparticles (DIO-NPs) with spherical shape and uniform size distribution as well as their accumulation and toxic effects on Jurkat cells up to 72 h. The characterization of dextran-coated maghemite nanoparticles was done by X-ray diffraction and dynamic light scattering analyses, transmission electron microscopy imaging, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, magnetic hysteresis, and relaxometry measurements. The quantification of DIO-NPs intracellular uptake showed a progressive accumulation of iron as a function of time and dose accompanied by additional lysosome formation and an increasing darkening exhibited by a magnetic resonance imaging (MRI) scanner. The cytotoxicity assays revealed a decrease of cell viability and a loss of membrane integrity in a time-and dose-dependent manner. Exposure to DIO-NPs determined an increase in reactive oxygen species level up to 72 h. In the first two days of exposure, the level of reduced glutathione decreased and the amount of malondyaldehyde increased, but at the end of the experiment, their concentrations returned to control values. These nanoparticles could be used as contrast agents for MRI but several parameters concerning their interaction with the cells should be taken into consideration for a safe utilization.

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“…Furthermore, the intensity of the bands corresponding to the maghemite γ-Fe 2 O 3 phase increases with increasing NPs concentration in the composite targets used in MAPLE experiments. The typical surface morphology of thin films deposited by MAPLE technique is characterized by an aggregated structure, consisting of micrometer-sized particles [17,18,22,39,119]. It is worth noting that a larger specific surface area was proven to induce an enhanced bioactivity, able to promote osteoblast differentiation, as reported in case of hybrid organic-inorganic thin films deposited by MAPLE [120,121].…”

Section: Hybrid Dextran-iron Oxide Thin Filmsmentioning

confidence: 81%

“…iron oxide, pointing to good biocompatibility [18]. Moreover, in a similar study, Ciobanu et al [39] showed that Hep G2 cells adhered very well to thin films of Dextran-doped maghemite and exhibited a normal actin cytoskeleton, proving that these cells underwent normal cell cycle progression. As a result, the authors consider that hepatocytes adhered to hybrid thin films could be used as biosensors for different xenobiotics.…”

Section: Hybrid Dextran-iron Oxide Thin Filmsmentioning

confidence: 82%

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Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Axente¹,

Sima²,

Ristoscu³

et al. 2016

Recent Advances in Biopolymers

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This chapter provides an overview of recent advances in the field of laser-based synthesis of biopolymer thin films for biomedical applications. The introduction addresses the importance of biopolymer thin films with respect to several applications like tissue engineering, cell instructive environments, and drug delivery systems. The next section is devoted to applications of the fabrication of organic and hybrid organic-inorganic coatings. Matrix-assisted pulsed laser evaporation (MAPLE) and Combinatorial-MAPLE are introduced and compared with other conventional methods of thin films assembling on solid substrates. Advantages and limitations of the methods are pointed out by focusing on the delicate transfer of bio-macromolecules, preservation of properties and on the prospect of combinatorial libraries' synthesis in a single-step process. The following section provides a brief description of fundamental processes involved in the molecular transfer of delicate materials by MAPLE. Then, the chapter focuses on the laser synthesis of two polysaccharide thin films, namely Dextran doped with iron oxide nanoparticles and Levan, followed by an overview on the MAPLE synthesis of other biopolymers. The chapter ends with summary and perspectives of this fast-expanding research field, and a rich bibliographic database.

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Biomedical properties and preparation of iron oxide-dextran nanostructures by MAPLE technique

Cited by 45 publications

References 50 publications

Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate

Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

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