Biometric and genetic differences in kelabau (Osteochilus spp.) as revealed using cytochrome c oxidase subunit 1

Abstract

Background: Kelabau (Osteochilus spp.) is a freshwater fish commonly found in the rivers of Riau, Indonesia. Researchers believe that these are Osteochilus kelabau; however, accurate taxonomic determination of these fish in Riau waters has not been made. The purpose of this study was to facilitate the identification of the kelabau based on its morphology and genetics using biometric and cytochrome c oxidase subunit 1 (CO1) analyses, respectively. Methods: Fish samples were collected from the Siak, Kampar and Rokan rivers in Riau Province, Indonesia. The DNA of 90 fish was extracted from the caudal fins using a DNA extraction kit, after which it was amplified using primers Fish-F1 and Fish-R1. Sequencing was conducted by Applied Biosystems Macrogen Korea, and the DNA sequences were then edited and aligned using MEGA v. 7. All samples were BLAST-searched for identification using the National Center for Biotechnology Information and BOLD System. Phylogenetic trees were constructed, and the similarity index was calculated using accession numbers AP011385.1 and KC631202.1 in GenBank. Results: Analysis of the consensus barcode sequence for 86 species revealed a high percentage of barcode matches (96%–97% in GenBank and 96.6%–96.76% in the BOLD System). The nucleotide distance between groups of kelabau from the different rivers based on the Kimura 2-parameter model gave the following results: 0.05% between groups from the Siak and Kampar rivers, 0.09% between those from the Siak and Rokan rivers and 0.05% between those from the Kampar and Rokan rivers. The nucleotide distance between the groups in the Siak (0.09%), Kampar (0.00%) and Rokan (0.10%) Rivers indicated that the kelabau in those rivers were related to each other. Conclusions: Based on the results of the research data using CO1 and biometric analyses, the kelabau were confirmed to be O. melanopleurus.

Dna Barcoding Using Coi Gene Sequences of Wild Betta Fighting Fish From Indonesia: Phylogeny, Status and Diversity

“…A total of 92 nucleotide sequences in this study were successfully read and aligned for further analyses. The analysis of 92 nucleotide sequences according to Tamura-Nei Model showed undistributed pattern of each nucleotide, namely Adenine (A) 25.08%, Thymine (T) 30.54%, Cytosine (C) 18.60%, and Guanine (G) 25.79% with pattern as follows: T > G > A > C. Several species had different pattern from the pattern obtained in this study, such as common carp with pattern of T > C > A > G (Mohanty et al, 2013) and kalabau fish with pattern of: T > A > C > G (Asiah et al, 2019). Estimation of substitution pattern between nucleotides was determined using the method of Maximum Composite Likelihood as presented in Table 2.…”

Genetic diversity analysis and phylogeography of Osteochilus spilurus (Cyprinidae: Labeoninae) from Bangka, Belitung, and Kalimantan Islands using Cytochrome b gene

“…The lowest Guanine than other nucleotides also found in molecular identification of O. spilurus(Kurniawan et al 2021b), Cyclocheilichthys apogon(Kenthao et al 2016) using cyt b gene, Osteochilus melanopleurus(Asiah et al 2020), Labeo rohita, Catla catla, Cirrhinus mrigala, Labeo fimbriatus, Labeo bata, and Cirrhinus reba(Mohanty et al 2015) using COI gene. When compared to covalent bonds in A-T bases, C-G base hydrogen bonds are weak, making them easily disrupted and leading to alterations in the fundamental molecular structure of DNA(Astuti et al 2020).…”

The Effect of Temperature on Osteochilus melanopleurus, Bleeker 1852 Yolk Utilization

“…Kelabau fish is one of consumption fish which found in the Riau rivers, Indonesia. This type of fish has been identified both molecularly and morphologically as Osteochilus melanopleurus [1]. Distribution of O. melanopleurus in Sumatera and Kalimantan which is included in the genus Osteochilus [2].…”