Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering

Arafat, M. Tarik; Lam, Christopher; Ekaputra, Andrew K.; Wong, Siew Yee; Li, Xu; Gibson, Ian

doi:10.1016/j.actbio.2010.09.010

Cited by 126 publications

(90 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[27][28][29] Meanwhile, the major organic component of bone ECM is collagen, which promotes early cell adhesion and interacts with osteoblasts. 28,30 The surface of the scaffolds plays an important role in interacting with host cells of tissue after implantation in situ.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic component coating on 3D scaffolds using high bioactivity of mesoporous bioactive ceramics

Yun¹,

Khang²,

Choi³

2011

IJN

View full text Add to dashboard Cite

Background Mesoporous bioactive glasses (MBGs) are very attractive materials for use in bone tissue regeneration because of their extraordinarily high bone-forming bioactivity in vitro. That is, MBGs may induce the rapid formation of hydroxy apatite (HA) in simulated body fluid (SBF), which is a major inorganic component of bone extracellular matrix (ECM) and comes with both good osteoconductivity and high affinity to adsorb proteins. Meanwhile, the high bioactivity of MBGs may lead to an abrupt initial local pH variation during the initial Ca ion-leaching from MBGs at the initial transplant stage, which may induce unexpected negative effects on using them in in vivo application. In this study we suggest a new way of using MBGs in bone tissue regeneration that can improve the strength and make up for the weakness of MBGs. We applied the outstanding bone-forming bioactivity of MBG to coat the main ECM components HA and collagen on the MBG-polycarplolactone (PCL) composite scaffolds for improving their function as bone scaffolds in tissue regeneration. This precoating process can also expect to reduce initial local pH variation of MBGs. Methods and materials The MBG-PCL scaffolds were immersed in the mixed solution of the collagen and SBF at 37°C for 24 hours. The coating of ECM components on the MBG-PCL scaffolds and the effect of ECM coating on in vitro cell behaviors were confirmed. Results The ECM components were fully coated on MBG-PCL scaffolds after immersing in SBF containing dilute collagen-I solution only for 24 hours due to the high bone-forming bioactivity of MBG. Both cell affinity and osteoconductivity of MBG-PCL scaffolds were dramatically enhanced by this precoating process. Conclusion The precoating process of ECM components on MBG-PCL scaffold using a high bioactivity of MBG was not only effective in enhancing the functionality of scaffolds but also effective in eliminating the unexpected side effect. The MBG-PCL scaffold-coated ECM components ideally satisfied the required conditions of scaffold in tissue engineering, including 3D well-interconnected pore structures with high porosity, good bioactivity, enhanced cell affinity, biocompatibility, osteoconductivity, and sufficient mechanical properties, and promise excellent potential application in the field of biomaterials.

show abstract

Section: Introductionmentioning

confidence: 99%

Biomimetic component coating on 3D scaffolds using high bioactivity of mesoporous bioactive ceramics

Yun¹,

Khang²,

Choi³

2011

IJN

View full text Add to dashboard Cite

show abstract

“…4), with a significant amount of extracellular matrix covering the fibers of the sheets. Previous studies have demonstrated that the biomimetic CaP layer supports the proliferation and adhesion of osteoblasts for long culture periods (23)(24)(25). Furthermore, MC3T3-E1 cells have been demonstrated to preferentially adhere to and/or proliferate in regions with a high CaP content (26).…”

Section: Resultsmentioning

confidence: 99%

Preparation and in vitro evaluation of a biomimetic nanoscale calcium phosphate coating on a polyethylene terephthalate artificial ligament

Chen

Guo

et al. 2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. In the present study, a polyethylene terephthalate (PET) artificial ligament was coated with an organic layer-by-layer (LBL) self-assembled template of chitosan and hyaluronic acid, and then incubated in a calcium phosphate (CaP) solution to prepare a biomimetic CaP coating. The surface characterization of the ligament was examined using scanning electron microscopy, atomic force microscopy and energy-dispersive X-ray spectroscopy. The effects of CaP coatings on the osteogenic activity of MC3T3 E1 mouse osteoblastic cells were investigated by evaluating their attachment, proliferation and the relative expression levels of alkaline phosphatase. The results revealed that the organic LBL template on the PET artificial ligament was effective for CaP apatite formation. Following incubation for 72 h, numerous nanoscale CaP apatites were deposited on the PET ligament fibers. In addition, the results of the in vitro culture of MC3T3-E1 mouse osteoblastic cells demonstrated that the CaP coating had a good biocompatibility for cell proliferation and adhesion, and the CaP-coated group had a significantly higher alkaline phosphatase activity compared with the uncoated control group after seven days of cell culture. Collectively, these results demonstrated that the biomimetic nanoscale CaP-coated PET artificial ligaments have potential in bone-tissue engineering.

show abstract

“…Interest in composites results from the biomimetic approach, which tries to understand and mimic the structure and mechanisms of natural tissue [1,6,31]. In tissue engineering, the most commonly used type of composite is [2,6,14,32].…”

Section: Biocomposites Applied For Tissue Engineeringmentioning

confidence: 99%

Scaffolds for Tissue Engineering

Chua¹,

Yeong²

2014

Bioprinting

View full text Add to dashboard Cite

A b s t r a c tThe paper presents the current trends in medicine of regenerative tissue defect caused by resection of tumors or fractures. Although it is a relatively young field of science, it creates new possibilities for the reconstruction of pathologically altered tissue with the use of three-dimensional scaffolds. Tissue engineering places particular emphasis on the type of scaffold from which they are made because of a number of requirements of medical materials including biocompatibility, mechanical strength and porosity. Keywords: scaffolds, tissue engineering, biomaterials S t r e s z c z e n i eArtykuł przedstawia trendy panujące w medycynie w regeneracji ubytków tkankowych powstałych na skutek resekcji nowotworów bądź złamań, skupione na wykorzystaniu inżynierii tkankowej. Ta stosunkowo młoda dziedzina nauki stwarza nowe możliwości odbudowy patologicznie zmienionych tkanek z wykorzystaniem trójwymiarowych rusztowań -skafoldów. Inżynieria tkankowa kładzie szczególny nacisk na rodzaj materiału, z jakiego produkowane są skafoldy, gdyż musi on spełniać szereg wymagań, m.in. biozgodność, wytrzymałość mechaniczna i porowatość.

show abstract

Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering

Cited by 126 publications

References 49 publications

Biomimetic component coating on 3D scaffolds using high bioactivity of mesoporous bioactive ceramics

Biomimetic component coating on 3D scaffolds using high bioactivity of mesoporous bioactive ceramics

Preparation and in vitro evaluation of a biomimetic nanoscale calcium phosphate coating on a polyethylene terephthalate artificial ligament

Scaffolds for Tissue Engineering

Contact Info

Product

Resources

About