Biomimetic fabrication of BCP/COL/HCA scaffolds for bone tissue engineering

Yang, Chun Rong; Wang, Y.J.; Chen, Xiaofeng; Zhao, Naru

doi:10.1016/j.matlet.2005.07.022

Cited by 25 publications

(10 citation statements)

References 32 publications

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“…On the other hand, bone regeneration was reported to be accelerated in the presence of ceramics, including synthetic calcium phosphate (BCP) and hydroxyl apatite (HAp), which makes them applicable for bone tissue engineering scaffolds . As ceramics are fragile, the applications have been limited to use with polymers …”

Section: Introductionmentioning

confidence: 99%

The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation

Amirian

Linh

Min

et al. 2014

J of Applied Polymer Sci

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A composite scaffold of gelatine (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was successfully fabricated. Growth factors such as bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were loaded into the Gel-Pec-BCP hydrogel scaffolds by freeze-drying. The surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive and X-ray diffraction spectroscopy. The results obtained from Fourier transform infrared spectroscopy and quantitative measurements showed successfully loading of BMP-2 and VEGF into the Gel-Pec-BCP hydrogel scaffolds. In addition MC3T3-E1 preosteoblasts were cultivated on the three types of scaffolds to investigate the effects of BMP-2 and VEGF on cell viability and proliferation. The Gel-Pec-BCP scaffolds loaded with VEGF and BMP-2 demonstrated more cell spreading and proliferation compared to those of the Gel-Pec-BCP scaffolds.

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Section: Introductionmentioning

confidence: 99%

The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation

Amirian

Linh

Min

et al. 2014

J of Applied Polymer Sci

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“…Nonetheless, the total augmented area of the ErhBMP-2/DBBC group was significantly higher at 8 weeks compared to that in the ErhBMP-2/CS and the Erh-BMP-2/FDBB groups (p<0.05). Previous studies have described that collagen coated alloplastic grafts facilitate the bone generation [43]. They interpreted that the role of collagen was to imbibe the blood easily and make the matrix formation reliable.…”

Section: Discussionmentioning

confidence: 99%

Effects of block bone substitutes loaded with Escherichia Coli-produced recombinant human bone morphogenetic protein-2 on space maintenance and bone formation in rat calvarial onlay model

Lee¹,

Jung²,

Pang³

2016

Tissue Eng Regen Med

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We aimed to evaluate the effects of onlay-type grafted human freeze-dried corticocancellous bone block (FDBB) and deproteinized bovine bone with collagen (DBBC) loaded with Escherichia coli-produced recombinant human bone morphogenetic protein-2 (ErhBMP-2) on space maintenance and new bone formation in rat calvaria. Collagen sponge (CS), FDBB, or DBBC disks (8×4 mm) with ErhBMP-2 (2.5 μg) were implanted onto the calvaria of male Sprague-Dawley rats, whereas CS with buffer was implanted onto the calvaria as controls (n=20/carrier). Rats were killed at 2 or 8 weeks post-surgery for histologic and histomorphometric analyses; total augmented area, new bone area, and bone density were evaluated. At both time-points, all ErhBMP-2 groups showed significantly higher new bone area and bone density than the control group (p<0.05). ErhBMP-2/FDBB and ErhBMP-2/DBBC groups showed significantly higher total augmented area than ErhBMP-2/CS group (8 weeks), and ErhBMP-2/FDBB group showed significantly higher new bone area and bone density than ErhBMP-2/DBBC group (p<0.05). ErhBMP-2/CS group showed the highest bone density (p<0.05). Combining ErhBMP-2 with FDBB or DBBC could significantly improve onlay graft outcomes, by new bone formation and bone density increase. Moreover, onlaygrafted FDBB and DBBC with ErhBMP-2 could be an alternative to autogenous block onlay bone graft.

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“…Besides, the peaks of 211, 112 and 300 overlapped with each other without division, suggesting that the crystallinity of the apatite crystals was very low. Compared with JCPDS standard card of HA, the peaks of the apatite crystals in the mineralized samples became wider, which indicated that the crystallite size was tiny and the crystallinity was very low, closely resembling that of the natural bone tissue [20,21]. Moreover, the diffraction peak at 2!…”

Section: Characterization Of Composite Membranesmentioning

confidence: 94%

Biomimetic synthesis of poly(lactic-co-glycolic acid)/multi-walled carbon nanotubes/apatite composite membranes

Zhang¹

2012

Express Polym. Lett.

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Abstract. Bioactive guided tissue regeneration (GTR) membrane has had some success for periodontal therapy. In this study, poly(lactic-co-glycolic acid) (PLGA)/multi-walled carbon nanotubes (MWNTs) composite membranes were incubated in three supersaturated calcification solutions (SCS) of different pH values for 21 days to prepare a PLGA/ MWNTs/apatite composite. Scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), energy dispersive spectroscopy (EDS), water contact angle measurement and mechanical testing were used for characterization. It was found that after 21 days incubation, apatite with low crystallite size and crystallinity was formed on the PLGA/MWNTs composite membranes. The Ca-poor carbapatite was similar in morphology and composition to that of natural bone. The size and shape of the apatite crystals immersed in three SCS were different from each other. The hydrophilicity and mechanical properties of the PLGA/MWNTs composite membranes were significantly enhanced after mineralization. This indicated that biomimetic mineralization may be an effective method to improve the biocompatibility and bone inductivity of certain materials. The PLGA/MWNTs/apatite composites may be potentially useful in GTR applications, particularly as GTR membranes for periodontal tissue regeneration.

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Biomimetic fabrication of BCP/COL/HCA scaffolds for bone tissue engineering

Cited by 25 publications

References 32 publications

The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation

The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation

Effects of block bone substitutes loaded with Escherichia Coli-produced recombinant human bone morphogenetic protein-2 on space maintenance and bone formation in rat calvarial onlay model

Biomimetic synthesis of poly(lactic-co-glycolic acid)/multi-walled carbon nanotubes/apatite composite membranes

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