Biomimetic hydroxyapatite/poly xylitol sebacic adibate/vitamin K nanocomposite for enhancing bone regeneration

Abstract

Hydroxyapatite (HAP) is a significant bone mineral that establishes bone strength. HAP composites in combination with biodegradable and bioactive polymer poly xylitol sebacic adipate (PXSA) would result in a constant release at target sites. Numerous studies have shown that vitamin K (VK) might possess a vital function in bone metabolism. The purpose of the present study was to inspect the synthesized composite HAP/PXSA/VK in developing polymeric biomaterials composite for the application of bone tissue regeneration. FTIR, X-ray diffraction, SEM and TEM techniques were applied to characterize the prepared composites. The release of VK from the HAP/PXSA/VK composite was evidenced through UV-Vis spectroscopy. In vitro studies proved that the HAP/PXSA/VK composite is appropriate for mesenchymal stem cell culture. Compared to pure HAP prepared following the same method, HAP/PXSA/ VK composite provided favourable microstructures and good biodegradation distinctiveness for the application of tissue engineering, as well as tissue in-growth characteristics and improved scaffold cell penetration. This work reveals that the HAP/PXSA/VK composites have the potential for applications in bone tissue engineering.

Nano-Hydroxyapatite Composite Scaffolds Loaded with Bioactive Factors and Drugs for Bone Tissue Engineering

“…The encapsulation efficiency of VK is about 80.3%, and the sustained release of VK is about 50.87% after 10 days. The HAp/PXSA/VK scaffold significantly promoted cell proliferation, adhesion and differentiation when MG63 was cultured on this scaffold [ 132 ]. Suzuki et al prepared polylactic acid/hydroxyapatite core-shell microspheres loaded with VK 1 , in which polylactic acid is the core and hydroxyapatite is the shell.…”

Recent Developments in Polymer Nanocomposites for Bone Regeneration

“…The HAP/PXSA/VK composite was suitable for MSC culture in vitro. The HAP/PXSA/VK composite had better microstructures and biodegradation characteristics than pure HAP synthesized using the same procedure [ 89 ]. The objective of regenerative medicine is to create synthetic biomaterials that have the inorganic and organic properties of real bone.…”

Combinatorial approach to fabricate silica doped polyvinyl alcohol/hydroxyapatite/carrageenan nanocomposite for bone regeneration applications

“…Such complications restrict their usage as artificial bone grafts. 3,[8][9][10][11] To overcome these shortfalls, bone tissue engineering (BTE) emerges as an attractive field that combines the basic biological properties and designing of various bone substitutes in order to ideally mimic the characteristics and functionalities of natural human bone. BTE focuses on designing and fabricating synthetic bone implants by combining variety of organic-inorganic biomaterials with appreciable bioactivities along with minimum toxicity and low bacterial infections.…”

“…1,9,[12][13][14][15] Hydroxyapatite Ca 10 (PO 4 ) 6 (OH) 2 has gained prodigious recognition in bone regeneration applications owing to its structural and characteristics resemblance with human bone. 8,16 As the basic inorganic constituent of bone, hydroxyapatite (HAp) exhibits superior biocompatibility, fine osteo-conductivity and osteo-inductivity. It is also known to improve cell adhesiveness and proliferation along with low immunogenicity and toxicity.…”

“…Inadequacy related to these conventional treatments including higher risk of infections, pain hemorrhage, donor site morbidity and immunogenic rejection. Such complications restrict their usage as artificial bone grafts 3,8–11 . To overcome these shortfalls, bone tissue engineering (BTE) emerges as an attractive field that combines the basic biological properties and designing of various bone substitutes in order to ideally mimic the characteristics and functionalities of natural human bone.…”