Biomolecular interaction study of hydralazine with bovine serum albumin and effect of <i>β</i>‐cyclodextrin on binding by fluorescence, 3D, synchronous, CD, and Raman spectroscopic methods

Bolattin, Mallavva B.; Nandibewoor, Sharanappa T.; Chimatadar, Shivamurti A.

doi:10.1002/jmr.2532

Cited by 10 publications

(7 citation statements)

References 32 publications

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“…The fluorescence intensity of peak ‘a’ was found to increase with the addition of complexes owing to the interaction between BSA and complexes 1 – 3 . That means that the diameter of the macromolecule increased and resulted in the increase of the scattering effect . Peak ‘b’ mainly represents the spectral behaviour of the tryptophan and tyrosine residues, the maximum emission wavelength and fluorescence intensity of which are closely correlated with the polarity of their microenvironments.…”

Section: Resultsmentioning

confidence: 83%

Newly synthesized Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes as potential anticancer candidates

İnci

Aydın

Huriyet

et al. 2018

Applied Organom Chemis

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pyrazino[2,3-f][1,10]phenanthroline; gly: glycine;tyr: tyrosine), have been synthesized and characterized using CHN analysis, electrospray ionization mass spectrometry, Fourier transform infrared spectroscopy and single-crystal X-ray diffraction. Interaction of these complexes with calf thymus DNA has been investigated using absorption spectral titration, ethidium bromide and Hoechst 33258 displacement assay and thermal denaturation measurements. These complexes were found to be efficient cleaving agents and cleavage reactions were mediated by hydrolytic and oxidative pathways. The interaction between these complexes and bovine serum albumin (BSA) was investigated using electronic absorption and fluorescence spectroscopy. The experimental results show that the fluorescence quenching mechanism of these complexes and BSA is a static quenching process. Furthermore, in vitro cytotoxicities of these complexes against tumour cell lines (Caco-2, MCF-7 and A549) and healthy cell line (BEAS-2B) showed that they exhibited anticancer activity with low IC 50 values. These complexes were markedly active against the cell lines and can be good drug candidates that are effective and safe for healthy tissue.

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Section: Resultsmentioning

confidence: 83%

Newly synthesized Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes as potential anticancer candidates

İnci

Aydın

Huriyet

et al. 2018

Applied Organom Chemis

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“…The fluorescence intensity of peak “a” was found to be increased with the addition of the complex. That is to say, the diameter of the macromolecule increased and concluded the increment of scattering effect . Peak “b” displays the spectral behavior of the tryptophan and tyrosine residues, the maximum emission wavelength and the fluorescence intensity of which are closely correlated with the polarity of their microenvironments.…”

Section: Resultsmentioning

confidence: 99%

Cu (II) tyrosinate complexes containing methyl substituted phenanthrolines: Synthesis, X‐ray crystal structures, biomolecular interactions, antioxidant activity, ROS generation and cytotoxicity

İnci

Aydın

Vatan

et al. 2018

Applied Organom Chemis

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In this paper, three new copper (II) complexes, [Cu(4-mphen)(tyr)(H 2 O)] ClO 4 (1), [Cu(5-mphen)(tyr)(H 2 O)]ClO 4 ·1.5H 2 O (2) and [Cu (tmphen)(tyr) (NO 3 )]0.5H 2 O (3) (4-mphen: 4-methyl-1,10-phenanthroline, 5-mphen: 5-methyl-1,10-phenanthroline, tmphen: 3,4,7,8-tetramethyl-1,10-phenanthroline and tyr: L-tyrosine), were synthesized and characterized using elemental analyses, FT-IR, ESI-MS, cyclic voltammetry and single-crystal X-ray diffraction. It was found that the complexes adopt a distorted five-coordinate square pyramidal geometry. The interaction of the three complexes with calf thymus DNA was also investigated using UV-visible absorption spectra, ethidium bromide and Hoechst 33258 displacement assay and thermal denaturation. The DNA cleavage activity of the complexes, monitored using gel electrophoresis, showed significant damage of the pUC19 plasmid DNA. Binding activity of bovine serum albumin (BSA) reveals that these complexes can strongly quench the fluorescence of BSA through a static quenching mechanism. The results suggested that interaction of the complexes with DNA occurred through a partial intercalation into the minor grooves of DNA. In addition, interaction of the complexes with bovine serum albumin quenched the fluorescence emission of the tryptophan residues of the protein binding constants and thermodynamic parameters were obtained from the fluorescence quenching experiments at different temperatures. Free radical scavenging activities of the complexes were determined by various in vitro assays such as 1,1-diphenyl-2-picryl-hydrazyl free radicals (DPPH˙) and H 2 O 2 scavenging methods. In addition, the cytotoxicity of these complexes in vitro on tumor cell lines (Caco-2 and MCF-7) was examined by XTT and showed better antitumor effect on the tested cells. ROS (reactive oxygen species) and comet experiments are consistent with each other and these complexes lead to DNA damage via the production of ROS. The effect of the hydrophobic properties of the synthesized complexes on DNA and BSA binding activities were discussed.

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“…Three‐dimensional fluorescence spectra and synchronous fluorescence spectra can provide information about the conformational changes of pepsin and trypsin with TF addition . For pepsin (Figure S1), as TF addition, the maximum peak (λ ex /λ em = 280/340 nm) was quenched by a ratio of 0.915 with a slightly red shift (Table ).…”

Section: Resultsmentioning

confidence: 99%

Investigation and comparison of the binding between tolvaptan and pepsin and trypsin: Multi‐spectroscopic approaches and molecular docking

Huang

et al. 2016

J of Molecular Recognition

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Tolvaptan (TF), a selective arginine vasopressin V2 receptor antagonist, was approved by the Food and Drug Administration in 2009. This study mainly investigated the differences between the binding of TF with pepsin and trypsin by using a series of spectroscopy and molecular modeling methods. Thermodynamic parameters and molecular docking results suggested that the binding of TF to pepsin and trypsin were both spontaneous but driven by different forces. For pepsin, the binding was driven by hydrogen bonds and van der Waals forces; but for trypsin, it was driven by electrostatic forces and hydrophobic forces. The quenching mechanism between TF and pepsin and trypsin was investigated by fluorescence experiments and time-resolved fluorescence spectroscopy. Synchronous fluorescence and 3-dimensional fluorescence were used to investigate the micro-environmental and conformational changes of pepsin and trypsin after the insertion of TF. In addition, activity-measurement results showed that both the pepsin and trypsin activities increased with increasing TF concentration, which may help to understand the possible effect of TF on the digestion and absorption of nutrients in vivo.

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Biomolecular interaction study of hydralazine with bovine serum albumin and effect of β‐cyclodextrin on binding by fluorescence, 3D, synchronous, CD, and Raman spectroscopic methods

Cited by 10 publications

References 32 publications

Newly synthesized Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes as potential anticancer candidates

Newly synthesized Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes as potential anticancer candidates

Cu (II) tyrosinate complexes containing methyl substituted phenanthrolines: Synthesis, X‐ray crystal structures, biomolecular interactions, antioxidant activity, ROS generation and cytotoxicity

Investigation and comparison of the binding between tolvaptan and pepsin and trypsin: Multi‐spectroscopic approaches and molecular docking

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