Bioorthogonal Turn‐On Probe Based on Aggregation‐Induced Emission Characteristics for Cancer Cell Imaging and Ablation

Abstract

Bioorthogonal turn-on probes have been widely utilized in visualizing various biological processes.Most of the currently available bioorthogonal turn-on probes are blue or green emissive fluorophores with azide or tetrazine as functional groups.H erein, we present an alternative strategy of designing bioorthogonal turn-on probes based on red-emissive fluorogens with aggregation-induced emission characteristics (AIEgens). The probe is water soluble and non-fluorescent due to the dissipation of energy through free molecular motion of the AIEgen, but the fluorescence is immediately turned on upon click reaction with azide-functionalized glycans on cancer cell surface.T he fluorescence turn-on is ascribed to the restriction of molecular motion of AIEgen, which populates the radiative decay channel. Moreover,t he AIEgen can generate reactive oxygen species (ROS) upon visible light (l = 400-700 nm) irradiation, demonstrating its dual role as an imaging and phototherapeutic agent.

Exploration of biocompatible AIEgens from natural resources

“…Since water soluble AIEgens have great potential in wash-free bio-imaging applications, 27 we thus explored the utilization of BBR chloride in living cell imaging. Before such an exploration, the cytotoxicity of BBR chloride was firstly evaluated using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay.…”

AIE Polymers: Synthesis, Properties, and Biological Applications

“…The nonradiative energy decays are thus blocked and strong emissions are observed . Due to the unique properties of AIE molecules, they have been widely used in chemo/biosensing, bioimaging, image‐guided therapy, and optoelectronic devices …”

A Very Short Be–Be Distance but No Bond: Synthesis and Bonding Analysis of Ng–Be2O2–Ng′ (Ng, Ng′=Ne, Ar, Kr, Xe)

“…After this study was completed, at heoretical work was published in continuationo fo ur earlier study of Be 2 B 8 and Be 2 B 7 À , [9] which reports very short BeÀBe distances between 1.728-1.866 in molecules with ar hombic Be 2 X 2 core (X = C, N). [23] The molecules have not been observed so far.L ately, at heoreticals tudy analyzed the bondings ituation of Be 2 O 2 . [24] The authors suggest that the rhombic D 2h speciesisana romatic species.…”