Biopreservation of Shrimps Using Composed Edible Coatings from Chitosan Nanoparticles and Cloves Extract

Abstract

Shrimps are highly valuable and perishable foodstuff that could be rapidly spoiled. Chitosan (Cht) was extracted and transformed into nanoparticles (NPs) via ionic gelation and fortified with cloves (Syzygium aromaticum) buds extract (CLE) for usage as antimicrobial composites against food-borne bacterial pathogens (Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus) and as edible coating (EC) for shrimp (Fenneropenaeus indicus) biopreservation throughout refrigerated storage. The synthesized CLE/Cht-NPs were electrostatically cross-linked and appeared with spherical shapes and homogenized distribution, with 159.4 nm mean size diameter and positive charge of 17.4 mV. The entire agents (Cht-NPs, CLE, and CLE/Cht-NPs) exhibited remarkable antibacterial activities toward all food-borne pathogens; CLE/Cht-NPs were significantly the most forceful. The scanning micrographs of treated S. typhimurium with CLE/Cht-NPs displayed NPs ability to attach and destroy bacterial cells. The ECs-treated shrimps exhibited sharp decrease in microbial groups load (aerobic microorganisms, E. coli, Enterobacteriaceae, and staphylococci) during refrigerated storage (4 ± 1°C) for 10 days. Additionally, the sensorial attributes (appearance, odor, color, and texture) of EC-treated samples preserved their elevated qualities for storage duration. The most effective EC blend contained 1.5% from Cht/NPs and 1.0% from CLE. The CLE/Cht-NPs could be impressively recommended as effectual natural composites for shrimps’ biopreservation during cold storage.

Application of Fish Collagen-Nanochitosan-Henna Extract Composites for the Control of Skin Pathogens and Accelerating Wound Healing

“…Accordingly, the compositing of these two powerful antimicrobials (NCht and He) led to forceful synergistic action that could effectually destroy the bacterial and yeast pathogens, in a time-dependent manner. This attribute was reported for NCht when incorporating with other bioactive phyto and natural compounds [20,24,41,58] 5 ND ND * "Inhibition zones impart triplicates' diameter means ± SD, assay discs (6 mm diameter) carried 50 μg from henna extract (He), nanochitosan (NCht), their blend (NCht/He), or standard antibiotic". * * "Dissimilar superscript letters within the same column indicated significant difference at p < 0:05.".…”

“…International Journal of Polymer Science particles, attachment to cells' membranes, interaction with intercellular components like DNA, and inhibition of their activities), but NCht particles have these attributes in superior manners, which make them ideal antimicrobial candidates [16,18,24,25]. The bioactivities of NCht (e.g., antimicrobial, anticancer, and preservative attributes) were illustrated to be augmented via nanopolymer incorporation with additional bioactive phytocompounds [18,26,41,58], which appointed the biocompatibility and synergy behavior of NCht when joining with other bioactive molecules.…”

“…Shrimp Shell Nanochitosan Preparation. Cts was extracted from shrimp (Penaeus monodon) waste exoskeletons, obtained from the "Aquaculture Research Farm, Kafrelsheikh University, Egypt", as demonstrated[41]. Briefly, shrimp flesh was detached; then, exoskeletons (shells) were washed repeatedly with DW and dried, at 50 ± 2°C for 22 h, and mechanically pulverized.…”

Innovative Approach for Controlling Black Rot of Persimmon Fruits by Means of Nanobiotechnology from Nanochitosan and Rosmarinic Acid-Mediated Selenium Nanoparticles

“…The Cht antimicrobial potentialities were demonstrated toward diverse microbial genera/species from bacteria, yeast or fungi [ 15 ]. The NPs formation from Cht (e.g., NCt) provided extra functionalities and bioactivities for this amazing biopolymer; the NCt was effectually employed as a potent antimicrobial, anticancer, drugs’ carrier, toxicant adsorbent, antioxidant, metals’ binder, anti-inflammatory, and nutraceuticals carrier [ 16 , 17 , 18 , 19 , 20 ].…”

In vitro antioxidant and antimicrobial activities of clove extract and its effectiveness in bio‐composite film on storage stability of goat meat balls

“…The antimicrobial packaging films might overcome this issue, wherein these films act as a delivery vehicle for reducing microflora on the surface of meat products and could be a safer option. Some studies have considered incorporating clove oil and its extract into the preparation of bio‐composite films to lower lipid oxidation and microbial growth (Hosseini et al., 2021; Liu et al., 2020; Tayel et al., 2020). However, reports on applying clove extract (CLE) as a natural preservative in goat meat balls through the bio‐composite film are scarce.…”