Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by <i>Halorubrum</i> sp. BS2

Sahli, Kaouther; Gomri, Mohamed Amine; Esclapez, Julia; Gómez‐Villegas, Patricia; Ghennai, Oulaya; Bonete, Marı́a José; Léon, Rosa; Kharroub, Karima

doi:10.1002/jobm.202000083

Cited by 33 publications

(27 citation statements)

References 40 publications

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“…As shown in Figure 4B, a decrease in the inorganic salt concentration in the culture medium induced the accumulation of carotenoids in cells, although the growth rate was reduced. This finding is consistent with that of previous reports on haloarchaea [28]. We have already published a study showing that 12.03% (w/v of NaCl) in the complex medium is optimum for carotenoid production in Hfx.…”

Section: Discussionsupporting

confidence: 93%

Haloferax mediterranei Cells as C50 Carotenoid Factories

et al. 2021

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Haloarchaea produce C50 carotenoids such as bacterioruberin, which are of biotechnological in-terest. This study aimed to analyze the effect of different environmental and nutritional conditions on the cellular growth and dynamics of carotenoids accumulation in Haloferax mediterranei. The maximum production of carotenoids (40 µg·mL−1) was obtained during the stationary phase of growth, probably due to nutrient-limiting conditions (one-step culture). By seven days of culture, 1 mL culture produced 22.4 mg of dry weight biomass containing 0.18 % (w/w) of carotenoids. On the other hand, carbon-deficient cultures (low C/N ratio) were observed to be optimum for C50 bacterioruberin production by Hfx. mediterranei, but negatively affected the growth of cells. Thus, a two-steps process was evaluated for optimum carotenoids yield. In the first step, a nutri-ent-repleted culture medium enabled the haloarchaea to produce biomass, while in the second step, the biomass was incubated under osmotic stress and in a carbon-deficient medium. Under the conditions used, the obtained biomass contained 0.27% (w/w) of carotenoids after seven days, which accounts for 58.49 µg·mL−1 of carotenoids for a culture with turbidity 14.0.

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Section: Discussionsupporting

confidence: 93%

Haloferax mediterranei Cells as C50 Carotenoid Factories

et al. 2021

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“…Recent studies report a moderate inhibitory effect of the extracts from the haloarchaea Halorubrum sp. on pathogenic bacteria, with inhibition halos up to 15 mm [ 24 ], which is quite lower than the results obtained is this work. Most studies about antimicrobial interactions between halophilic bacteria and archaea are focused on halocins [ 78 , 79 , 80 ].…”

Section: Resultscontrasting

confidence: 64%

“…Examples of this are the red pigment prodigiosin, isolated from the sponge-associated bacterium Serratia marcescens [ 21 ]; or the chlorophyll a derivatives obtained from the microalga Isochrysis galbana [ 22 ]. Recently, the antimicrobial activity of pigments from some halophilic bacteria has been tested against bacteria and fungi [ 23 ]; however, so far, the studies about the antimicrobial activity of haloarchaeal pigments or extracts thereof are scarce [ 19 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)

Gómez‐Villegas

Vigara

Vilà

et al. 2020

Biology

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The need to survive in extreme environments has furnished haloarchaea with a series of components specially adapted to work in such conditions. The possible application of these molecules in the pharmaceutical and industrial fields has received increasing attention; however, many potential bioactivities of haloarchaea are still poorly explored. In this paper, we describe the isolation and identification of two new haloarchaeal strains from the saltern ponds located in the marshlands of the Odiel River, in the southwest of Spain, as well as the in vitro assessment of their antioxidant, antimicrobial, and bioactive properties. The acetone extract obtained from the new isolated Haloarcula strain exhibited the highest antioxidant activity, while the acetone extracts from both isolated strains demonstrated a strong antimicrobial activity, especially against other halophilic microorganisms. Moreover, these extracts showed a remarkable ability to inhibit the enzyme cyclooxygenase-2 and to activate the melanogenic enzyme tyrosinase, indicating their potential against chronic inflammation and skin pigmentation disorders. Finally, the aqueous protein-rich extracts obtained from both haloarchaea exhibited an important inhibitory effect on the activity of the acetylcholinesterase enzyme, involved in the hydrolysis of cholinergic neurotransmitters and related to several neurological diseases.

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“…Carotenoids from Halorubrum sp. BS2 reported having extraordinary antioxidant capacity than that of the ascorbic acid, a standard antioxidant [48]. The antioxidant capabilities of carotenoids produced by Haloterrigena turkmenica, Haloferax volcanii, Halococcus morrhuae, Halogranum rubrum, and Halobacterium salinarum were significantly higher than the standards β-carotene [43].…”

Section: Haloferax Volcaniimentioning

confidence: 94%

“…Through partial 16S rRNA sequencing, these strains were recognized to belong to Haloferax (Hfx) sp. [48]. [56] 3.3 Bacteria…”

Section: Antimicrobial Activity Of Archaeamentioning

confidence: 99%

Microorganisms: A Potential Source of Bioactive molecules for Antioxidants and Antimicrobial Applications

Rani¹,

Saini²,

Bast³

et al. 2021

Preprint

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Oxidative stress is an elevated intracellular level of free oxygen radicals that cause lipid peroxidation, protein denaturation, DNA hydroxylation, and apoptosis, ultimately negotiating cells viability. Antioxidants can scavenge such free radicals, thus reducing the oxidative stress and eventually prevent cellular damage. Medicinal plants, fruits, and spices remain the prioritized sources of antioxidants and antimicrobial properties since the time immemorial, but in contrast to plants, microorganisms can be grown at a faster rate under controlled conditions. They are non-toxic, non-carcinogenic, and biodegradable as compared to synthetic antioxidants. Microorganisms including actinomycetes, archaea, bacteria, protozoa, yeast, and fungi are auspicious source of vital bioactive compounds. The list comprises ample of bioactive components from microorganisms. One of them is bacteriocins, which are ribosomally synthesized antimicrobial peptides product of Eurotium sp., Streptomyces parvulus, S. thermophiles, Lactococcus lactis, etc. It has a great potential as next-generation antibiotics targeting the multiple-drug resistant pathogens. Pneumocandins are antifungal lipohexapeptides derived from the fungus Glarea lozoyensis, and inhibit 1,3-β-glucan synthase of the fungal cell wall and act as a precursor for the synthesis of caspofungin. It is widely used against invasive fungal infections and has been recently approved by the FDA. Taxol (paclitaxel), a chemotherapeutic drug derived from the bark of Taxus brevifolia can also be produced by endophytic fungi Taxomyces andreanae and Nodulisporium sylviforme. It is known to inhibit several fungi such as Pythium, Aphanomyces and Phytophthora. Hispidin and its derivate isolated from P. hispidus, reduce inducible nitric oxide synthase (iNOS) expression, obstruct the transcriptional activity of NF-κB, and also decrease the production of reactive oxygen species (ROS) in macrophages. Astaxanthin, known as an “aquatic” carotenoid produced by H. pluvialis, also has excellent ROS quenching activity. This study mainly focuses on fascinating antioxidant and antimicrobial compounds that have been scarcely investigated in microorganisms and discuss the promise and challenges of microorganisms as providers of health benefits.

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Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by Halorubrum sp. BS2

Cited by 33 publications

References 40 publications

Haloferax mediterranei Cells as C50 Carotenoid Factories

Haloferax mediterranei Cells as C50 Carotenoid Factories

Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)

Microorganisms: A Potential Source of Bioactive molecules for Antioxidants and Antimicrobial Applications

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