Bioreactors for tissue mass culture: Design, characterization, and recent advances

Martin, Yves; Vermette, Patrick

doi:10.1016/j.biomaterials.2005.05.057

Cited by 341 publications

(268 citation statements)

References 117 publications

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“…The success of cell attachment to the scaffold depends on the flow regime. High flows cause high shear stress (Martin and Vermette 2005) and prevent attachment of cells to the scaffold surface, already attached cells may be damaged or hindered in proliferation. Shear stress is also considered as an important parameter for in vivo systems.…”

Section: Introductionmentioning

confidence: 99%

Darcian permeability constant as indicator for shear stresses in regular scaffold systems for tissue engineering

Vossenberg

Higuera

Straten

et al. 2009

Biomech Model Mechanobiol

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The shear stresses in printed scaffold systems for tissue engineering depend on the flow properties and void volume in the scaffold. In this work, computational fluid dynamics (CFD) is used to simulate flow fields within porous scaffolds used for cell growth. From these models the shear stresses acting on the scaffold fibres are calculated. The results led to the conclusion that the Darcian (k 1 ) permeability constant is a good predictor for the shear stresses in scaffold systems for tissue engineering. This permeability constant is easy to calculate from the distance between and thickness of the fibres used in a 3D printed scaffold. As a consequence computational effort and specialists for CFD can be circumvented by using this permeability constant to predict the shear stresses. If the permeability constant is below a critical value, cell growth within the specific scaffold design may cause a significant increase in shear stress. Such a design should therefore be avoided when the shear stress experienced by the cells should remain in the same order of magnitude.

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Section: Introductionmentioning

confidence: 99%

Darcian permeability constant as indicator for shear stresses in regular scaffold systems for tissue engineering

Vossenberg

Higuera

Straten

et al. 2009

Biomech Model Mechanobiol

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“…However, the cell dose is below the standards defined in the current guidelines for transplantation (Bornstein et al 2005). The National Aeronautics and Space Administration (NASA) developed two RWV bioreactors for tissue mass culture (Martin and Vermette 2005). The slow turn lateral vessel (STLV) bioreactor has been used to culture several kinds of cells both on Earth and in space.…”

Section: Rotating Wall Vesselsmentioning

confidence: 99%

Expansion of human hematopoietic stem cells for transplantation: trends and perspectives

2008

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Umbilical cord blood transplantation is clinically limited by its low progenitor cell content. Ex vivo expansion has become an alternative to increase the cell dose available for transplants. Expansion has been evaluated in several ways such as static cultures combining growth factors or mimicking the natural microenvironment using co-culture systems. However, static cultures have a small volume capacity and therefore large-scale expansion has been addressed using bioreactors. These and other biotechnological approaches for the expansion of hematopoietic progenitors and their utility to study several aspects of hematopoietic stem cell biology are discussed here.

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“…The use of bioreactors, allows users to maintain the specific environmental conditions necessary for the proper survival of the tissue that is being cultivated (Griffith and Naughton, 2002;Martin et al, 2004;Martin and Vermette, 2005). Due to the fact that tissues, unlike cellular monolayers, need to grow in multiple layers, they begin to form a thickness and three-dimensional structure.…”

Section: Introductionmentioning

confidence: 99%

Growth rates of dynamic dermal model exposed to laminar flow and magnetic fields

et al. 2016

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Introduction: Ongoing research in the use of electromagnetic stimulation as coadjuvant in fracture healing has led the authors to begin generating computer models in order to predict cellular growth changes when cells are electromagnetically stimulated. By generating these models, scientists will be able to better understand how electromagnetic fields affect cellular development. The experimental design integrated a cellular culture bioreactor along with an external magnetic stimulation system, which allowed for dermal models to be exposed to controlled magnetic fields. Methods: Initially, it was necessary to analyze the static growth of Normal Human Skin Fibroblast (NHSF) cells when they were exposed to Extremely Low Frequency -Electromagnetic Fields (ELF-EMFs). Using optimal conditions for the NHSF culture, from stimulation signal to scaffolding material, we were able to perform the dynamic flow stimulation experiments. Results: The following systems were developed: (1) a bioreactor aimed at cellular tissue culture, and (2) Helmholtz coils capable of generating stimulation signals for the cultured tissue. The authors were able to appreciate the quantified values of cellular density diluted in all the experiment samples that were taken and overall, the irradiated samples displayed an average increase of 53% higher cellular density for the same amount of initial cellular seeding when the cells were exposed to a 1 mT, 60 Hz magnetic field signal. Conclusion: ELF-EMF's indeed alter NHSF cell growth rates and it is the challenge of the authors to continue investigating what cellular mechanisms are altered when cells are exposed to ELF-EMF's.

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Bioreactors for tissue mass culture: Design, characterization, and recent advances

Cited by 341 publications

References 117 publications

Darcian permeability constant as indicator for shear stresses in regular scaffold systems for tissue engineering

Darcian permeability constant as indicator for shear stresses in regular scaffold systems for tissue engineering

Expansion of human hematopoietic stem cells for transplantation: trends and perspectives

Growth rates of dynamic dermal model exposed to laminar flow and magnetic fields

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