Biosilica-enveloped ferritin cage for more efficient drug deliveries

Ki, Mi-Ran; Nguyen, Thi Khoa My; Jun, Hyun Sik; Pack, Seung Pil

doi:10.1016/j.procbio.2018.02.020

Cited by 26 publications

(12 citation statements)

References 54 publications

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“…27 Therefore, ferritin has a number of successful applications in bionanomaterial preparation, drug transport, and nanoreactors. 28 Herein, we proposed an all-in-one strategy to solve the above-mentioned three problems by fusing the coding gene of bovine carbonic anhydrase (bCA) with ferritin (F). The recombinant bCA-F self-assembled into microsized supraparticles and was efficiently purified by low-speed centrifugation in high activity recovery and yield.…”

Section: ■ Introductionmentioning

confidence: 99%

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Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Liu

Yuan

et al. 2022

ACS Sustainable Chem. Eng.

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Carbon dioxide capture, conversion, and utilization with carbonic anhydrase (CA) as a green and sustainable method still faces the challenges of complex and costly purification, relatively low yield, and poor catalytic performances and reusability. Herein, we proposed an all-in-one strategy to solve these problems by self-assembling nanosized CA oligomeric particles (nCAOPs) into micrometer-sized CA supraparticles (mCASPs) with well-designed tags. The preparation of the purified mCASPs was simple and effective by an easy scalable low-speed centrifugation method with 78% activity recovery. The obtained mCASPs with a yield of 870 mg/L was the reported highest yield of CAs. Interestingly, mCASPs could serve as carrier-free immobilized CAs and retain over 90% original activity after 15 reuse cycles. More encouragingly, mCASPs could redissolve and form nCAOPs, which had excellent catalytic performances. The hydrated activity of nCAOPs was 1.05 times that of free CAs. Also, the k cat/K m value was 1.74 times that of free CAs, and the half-life at 40 °C was 3.83 times that of free CAs. Due to the simplicity of purification and immobilization, high yield, and excellent enzymatic properties, mCASPs and nCAOPs are considered novel bioactive materials, which offer the feasibility of CAs for sustainable CO2 capture to achieve the target of carbon neutrality.

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Section: ■ Introductionmentioning

confidence: 99%

“…Ferritin has a strictly uniform size and shape and is capable of forming ordered nanostructures by self-assembly . Therefore, ferritin has a number of successful applications in bionanomaterial preparation, drug transport, and nanoreactors …”

Section: Introductionmentioning

confidence: 99%

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Liu

Yuan

et al. 2022

ACS Sustainable Chem. Eng.

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“…Interestingly, the Pack group established an innovative system for drug delivery by integrating two biocompatible approaches, Fn nanocages and bioinspired silicification ( Figure 1 ) [ 26 ]. As a result, the surface of Fn nanoparticles was genetically modified by presenting a short silica-forming peptide (SFP) on its surface, which could direct the deposition of biosilica around chimeric Fn fusion proteins [ 49 , 50 ].…”

Section: Engineered Design Of Naturally Occurring Self-assembled Prot...mentioning

confidence: 99%

“…The silica-forming peptide (silaffin R5) tagged Fn fusion protein was self-entrapped in biosilica, acting as a nanocarrier for ( A ) one-drug or ( B ) two-drug delivery. ( A ): Reproduced with permission from [ 26 ], Copyright 2018 Elsevier Ltd. ( B ): Reproduced with permission from [ 27 ], Copyright 2019 The Korean Society of Industrial and Engineering Chemistry (Elsevier).…”

Section: Figurementioning

confidence: 99%

Tailored Functionalized Protein Nanocarriers for Cancer Therapy: Recent Developments and Prospects

Abdel‐Hamid

El-Hafeez

et al. 2023

Pharmaceutics

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Recently, the potential use of nanoparticles for the targeted delivery of therapeutic and diagnostic agents has garnered increased interest. Several nanoparticle drug delivery systems have been developed for cancer treatment. Typically, protein-based nanocarriers offer several advantages, including biodegradability and biocompatibility. Using genetic engineering or chemical conjugation approaches, well-known naturally occurring protein nanoparticles can be further prepared, engineered, and functionalized in their self-assembly to meet the demands of clinical production efficiency. Accordingly, promising protein nanoparticles have been developed with outstanding tumor-targeting capabilities, ultimately overcoming multidrug resistance issues, in vivo delivery barriers, and mimicking the tumor microenvironment. Bioinspired by natural nanoparticles, advanced computational techniques have been harnessed for the programmable design of highly homogenous protein nanoparticles, which could open new routes for the rational design of vaccines and drug formulations. The current review aims to present several significant advancements made in protein nanoparticle technology, and their use in cancer therapy. Additionally, tailored construction methods and therapeutic applications of engineered protein-based nanoparticles are discussed.

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“…After showing that the transport protein ferritin can be modified with a silica-binding peptide to create a silica-enveloped drug carrier, Ki et al proved that a second drug can be loaded into the outer silica shell. 217,218 The resulting dual delivery system was tested in vitro, revealing that the two drugs can be released at different rates after cellular intake. Modified ferritin was also used for the controlled synthesis of silica nanoparticles with an encapsuled anticancer drug.…”

Section: Silicatesmentioning

confidence: 99%

A sticky tale : Biomaterial coatings from modular proteins

Alvisi¹

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Biosilica-enveloped ferritin cage for more efficient drug deliveries

Cited by 26 publications

References 54 publications

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Tailored Functionalized Protein Nanocarriers for Cancer Therapy: Recent Developments and Prospects

A sticky tale : Biomaterial coatings from modular proteins

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Biosilica-enveloped ferritin cage for more efficient drug deliveries

Cited by 26 publications

References 54 publications

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO2 Capture

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO2 Capture

Tailored Functionalized Protein Nanocarriers for Cancer Therapy: Recent Developments and Prospects

A sticky tale : Biomaterial coatings from modular proteins

Contact Info

Product

Resources

About

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture