Biosurfactant Production by Streptomyces sp. CGS B11 Using Molasses and Spent Yeast Medium

Abstract

Biosurfactants are considered a good alternative to highly-polluting petroleum-based surfactants that are toxic and non-biodegradable in nature. However, the high production cost of biosurfactants limits its potential for commercialization. The use of a highly efficient biosurfactant-producing actinomycetes isolate, combined with the utilization of low-cost substrates such as agro- industrial wastes, may aid in lowering the overall production cost. In this study, twenty-eight (28) actinomycetes isolated from distillery wastes and soil samples were screened for the production of extracellular biosurfactants. Based on the preliminary screening experiment, isolate CGS B11 – molecularly identified as Streptomyces angustmyceticus – produced the biosurfactant with the highest emulsification activity (E24). Subsequently, the best alternative carbon and nitrogen sources, salt supplement, and pH level were determined using one-factor-at-a-time (OFAT) experiments. The highest measured biosurfactant activity was observed in the medium containing molasses, spent yeast autolysate, and NaCl, and a pH level ranging from 6.0–7.0. Biosurfactant production was observed to be growth associated with maximum emulsification activity achieved after 4 d of fermentation (late log phase). FTIR (Fourier transform infrared) spectra and biochemical composition analyses of the S. angustmyceticus CGS B11 biosurfactant suggest that it belongs to the lipopeptide type of biosurfactants. S. angustmyceticus CGS B11 biosurfactant also showed resistance and high stability on a wide range of temperature, pH, and salinity, and the ability to form stable and dense emulsions with various oils tested. It has great potential for various applications such as in the food and pharmaceutical industries, and in oil recovery and bioremediation. The results of this study will hopefully serve as a basis for large-scale production of biosurfactants utilizing agro-industrial wastes in the country.

A new bacterial-derived biosurfactant for biotechnological applications in the oil industry: production, optimization, biosurfactant functional and physicochemical characterization

“…These results are of growing interest as the prospective application of BioS in numerous industrial processes necessitates thermal stabilities. Previous research works exhibited similar findings(Chen et al 2017;Ghazala et al 2017;Kiran et al 2017;Martins et al 2018;Feng et al 2019;Purwasena et al 2019;Zouari et al 2019;Jimenez et al 2021;Mnif et al 2021aMnif et al , 2021bUmar et al 2021;Wu et al 2022).…”

“…Similar results are observed for emulsifying activity with higher attenuation than foaming power. These results go in favor of broadening its purview of use in different areas, especially for the enhanced oil recovery and biological remediation of sea water contamination (Chen et al 2017;Ghazala et al 2017;Kiran et al 2017;Martins et al 2018;Feng et al 2019;Purwasena et al 2019;Zouari et al 2019;Jimenez et al 2021;Mnif et al 2021aMnif et al , 2021bUmar et al 2021;Wu et al 2022).…”

Avaliação da produção de biossurfactantes por Pseudomonas fluorescens em substrato de melaço como fonte única de Carbono

“…Alta concentração de sacarose, glicose e frutose foram obtidas, evidenciando-se a disponibilidade de nutrientes para o crescimento microbiano. Por outro lado, concentrações muito altas de açúcares podem inibir a produção de biossurfctantes por excesso de substrato (Joshi et al, 2008;Jimenez et al, 2020), fato que deixou clara a necessidade de diluição do melaço, de maneira a torná-lo apto para o objetivo em questão. Estudos sugerem que concentrações em torno de 20 a 70 g/L (o equivalente a 2% m/v -7% m/v) de melaço oferecem boa fonte nutritiva para produção dos biotensoativos sem causar inibição.…”

Combination of Mollase and Glucose as Substrate for The Production of Biosurfactant by Bacillus subtilis BK7.1

“…The platinum ring was submerged right on the surface of supernatant and then pulled back slowly. The surface activities of the biosurfactants were determined by measuring the surface tension using Du Nouy's Tensiometer (Ogawa Seiki Co., Ltd) using the ring method at room temperature (25°C) [14].…”