Biosynthesis and Characterization of Silver Nanoparticles (Snps) by using Leaf Extracts of Ocimum Sanctum L (Tulsi) and Study of its Antibacterial Activities

Abstract

The present study aimed to synthesis of SNPs (Silver Nanoparticles) or (Green-Silver) in aqueous medium using leaf extracts of Ocimum sanctum L. The synthesized silver nanoparticles (SNPs) were characterized by the different techniques such as UV-vis Spectrophotometer, XRD, FTIR and TEM. The synthesis of silver nanoparticles were confirmed due to the SPR optical absorption band peak at ~440 nm by UV-vis spectrophotometer, nearly 15-45 nm in diameter with spherical in shape by TEM, high crystalline with the Bragg peaks of (111), (200), (220) and (311) plane as the predominant orientation by XRD and FTIR confirmed the presence of methoxy and allyl groups in SNPs. The reduction of Ag metal ions was performed due to the presence of various types of anti-oxidants in the leaves of Ocimum sanctum L. and each having a unique structure and functions. The formations of silver nanoparticles through aqueous medium were fairly stable and which can be incubated up to 4 months in refrigerator. At last, the antimicrobial activities of AgNPs were evaluated using different bacteria strain. It was found that, this biosynthesizing of silver nanoparticles may inhibit the all types of bacterial pathogenic organisms competently. This recent research study opens an innovative design to progress our understanding of how SNPs behave can be optimized to improve human antimicrobial activities.Citation: Bindhani BK, Panigrahi AK (2015) Biosynthesis and Characterization of Silver Nanoparticles (SNPs) by using Leaf Extracts of Ocimum Sanctum L (Tulsi) and Study of its Antibacterial Activities.

Synthesis of silver nanoparticles (AgNPs) from leaf extract of Salvia miltiorrhiza and its anticancer potential in human prostate cancer LNCaP cell lines

“…Nanotechnology is the field of materials science to develop the most active nanomaterials for the therapeutic potential of numerous diseases [1]. The prepared nanoparticles exhibits several physiochemical properties and characteristics such as size, morphology and distribution [2].…”

“…Silver has been recognized to inhibit microbes for medical and industrial application and it has been mainly used in the medical and pharmaceutical industry for preparing topical ointments to prevent infection against burn and wounds. Moreover, silver metal has been considered as a toxic material that can cause deleterious toxicity against several types of cancer cells [1]. Synthesis and characterization of silver nanoparticles from the medicinal plant or extracts are recently shown to be the greatest approach to develop selective drug delivery for cancer treatment.…”

Biomimetic Synthesis and Antibacterial Properties of Strontium Oxide Nanoparticles Using Ocimum Sanctum Leaf Extract

“…These phenolic compounds have pharm logical, medicinal, antibacterial, and an antioxidant effect [21]. Bindhani and Panigrahi suggested that the reducing property of the OS leaf extract was associated with their specific phenolic composition such as eugenol [22]. Recently, Apsana et al also reported the reduction of Ca and Co metal ions using OS leaf extract and further it is confirmed that the possible reducing agent is eugenol by characterizing OS leaf extract using ultraviolet (UV) spectral analysis [23].…”

Quercetin-sensitized BiOF nanostructures: An investigation on photoinduced charge transfer and regeneration process for degradation of organic pollutants

“…Among the various green techniques, the use of plant leaf extract provides an economic viable and ecofriendly path for a well-regulated synthesis of nanostructures with well-defined morphology [26,27]. Until now, various plant extracts such as P. trifoliate fruit extract [28], Ocimum Sanctum L (Tulsi) [29], Ocimum Tenuiflorum (black Tulsi) [30] and Ficus benghalensis (Banyan tree) [31] etc. have been utilized for the preparation of nanostructures.…”