Biosynthesis and Metabolism of Plant Hormones

Sembdner, G.; Gross, D.; Liebisch, H.-W.; Schneider, G.

doi:10.1007/978-3-642-67704-5_5

Cited by 132 publications

(67 citation statements)

References 1,006 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fractions (Ve = 45 ± 5 ml), the UV spectra of which were most similar to that of authentic IAA methyl ester, were pooled. The following approximate quantities of IAA methyl ester (estimated by UV absorbance at 289, 279, and 219 nm) were thus obtained from these species: Lemna trisulca, 890,ug; Leucobryum (16,23) and IAA to indole-3-carboxylic acid (23). For these reasons, we can assume with confidence that the indolic compounds, detected with our methods, which accumulate only in plants supplied with tryptophol, are metabolites of that tryptophol.…”

mentioning

confidence: 62%

“…It is also formed in vitro by preparations from bacteria, thallophytes, and seed plants from compounds supposed to participate in the biogenesis of IAA (e.g. 18,23). In fact, tryptophol appears to be in an oxidase-reductasemediated equilibrium with the IAA precursor, indole-3-acetaldehyde (5, 16).…”

Section: Introductionmentioning

confidence: 99%

“…Indole-3-acetaldehyde was never detected; the aldehyde has only rarely been isolated from plant systems (23) Of 67 values reported, 48 were between 10 and 300 ng/g fresh weight. The lowest and the highest concentrations reported were 2 and 3,000 ng/g.…”

mentioning

confidence: 99%

“…IAA, if detectable at all, was usually a minor metabolite, as should be expected, if tryptophol oxidase responds to feedback inhibition by IAA. Indole-3-carboxylic acid, formed by a few fungi and mosses, was the only tryptophol metabolite detected which is likely to be formed via IAA.Indole-3-ethanol (tryptophol2) has been established as an endogenous plant constituent (6,19,23). It is also formed in vitro by preparations from bacteria, thallophytes, and seed plants from compounds supposed to participate in the biogenesis of IAA (e.g.…”

mentioning

confidence: 99%

“…Indole-3-ethanol (tryptophol2) has been established as an endogenous plant constituent (6,19,23). It is also formed in vitro by preparations from bacteria, thallophytes, and seed plants from compounds supposed to participate in the biogenesis of IAA (e.g.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Metabolism of Tryptophol in Higher and Lower Plants

Laćan¹,

Magnus²,

Šimaga³

et al. 1985

Plant Physiol.

View full text Add to dashboard Cite

Bacteria, thallophytes, and seed plants (107 species), supplied with exogenous indole-3-ethanol (tryptophol), formed one or more of the following metabolites: O-acetyl tryptophoL an unknown tryptophol ester (or a set of structurally closely related esters), tryptophol glucoside, tryptophol plactoside, indole-3-acetic acid (IAA), and indole-3-carboxylic acid. The unknown ester was formed by all species exAmined; 0-acetyl tryptophol appeared sporadically in representatives of most major taxonomic groups. Tryptophol galactoside was found in the algae Chlorella, Euglena, and Ochromoaas. The glucoside was formed by many eucaryotic plants, but not by bacteria; it was a significant tryptophol metabolite in vascular plants. IAA, if detectable at all, was usually a minor metabolite, as should be expected, if tryptophol oxidase responds to feedback inhibition by IAA. Indole-3-carboxylic acid, formed by a few fungi and mosses, was the only tryptophol metabolite detected which is likely to be formed via IAA.Indole-3-ethanol (tryptophol2) has been established as an endogenous plant constituent (6,19,23). It is also formed in vitro by preparations from bacteria, thallophytes, and seed plants from compounds supposed to participate in the biogenesis of IAA (e.g. 18,23). In fact, tryptophol appears to be in an oxidase-reductasemediated equilibrium with the IAA precursor, indole-3-acetaldehyde (5, 16). The oxidase has been isolated and found to be feedback inhibited by IAA (16). This points to the involvement of tryptophol in a mechanism stabilizing auxin levels in plants. Its in vivo significance has, however, remained unclear, because IAA concentrations necessary to inhibit the isolated tryptophol oxidase are larger than bulk concentrations of the hormone in plant tissues (16).Local IAA concentrations at points ofregulation may be higher than bulk concentrations. Also, removal of tryptophol oxidase from its natural subcellular environment may render the enzyme less sensitive to feedback inhibitors. For these reasons, we were looking for indications of regulated IAA formation from tryptophol in systems containing nondisrupted cell structures. Suspensions of unicellular plants and sections of multicellular plants were supplied with a large excess of tryptophol. In the absence of regulatory mechanisms, this might reasonably be expected to lead to drastically enhanced IAA levels. However, the majority of plants examined did not accumulate substantial amounts of 2 Abbreviations: tryptophol, indole-3-ethanol or 3-(2-hydroxyethyl)indole; Ve, elution volume.The most abundant tryptophol metabolites in bacteria, thallophytes, and seed plants were conjugates: esters and glycosides.MATERIALS AND METHODS Instrumentation. 'H NMR spectra were recorded at 100 MHz on a JEOL FX-100 Fourier-transform spectrometer. Chemical shifts (a) were recorded relative to tetramethylsilane. GC-MS was on a Hewlett-Packard 5985a instrument, using helium at 30 ml/ min as the carrier gas and electron impact ionization at 70 ev. Columns and temperature pr...

show abstract

mentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Metabolism of Tryptophol in Higher and Lower Plants

Laćan¹,

Magnus²,

Šimaga³

et al. 1985

Plant Physiol.

View full text Add to dashboard Cite

show abstract

Anatomical, Physiological, and Hormonal Aspects of Abscission in Citrus

Gören

1993

Horticultural Reviews

View full text Add to dashboard Cite

Gas chromatographic/mass spectrometric and tandem mass spectrometric investigations of synthetic amino acid conjugates of jasmonic acid and endogenously occurring related compounds fromVicia faba L

Schmidt¹,

Kramell²,

Brückner³

et al. 1990

Biol. Mass Spectrom.

View full text Add to dashboard Cite

Some partial synthetic amino acid ((S)‐alanine, (S)‐leucine, (S)‐isoleucine, (S)‐aspartic acid, (S)‐glutamic acid, (S)‐phenylalanine, (S)‐ and (R)‐valine) conjugates of the plant growth regulator (‐)‐jasmonic acid (JA) were investigated as methyl esters by capillary gas chromatography/mass spectrometry. By gas chromatographic data (retention indices) as well as by mass spectral behaviour all these substances can be differentiated unequivocally. In addition, the distinction between the leucine and isoleucine JA conjugates was carried out by mass‐analysed ion kinetic energy spectroscopy (tandem mass spectrometry). Based on this some naturally occurring (S)‐isoleucine conjugates of (‐)‐JA, (+)‐7‐iso‐JA and related compounds as well as the (S)‐leucine and (S)‐phenylalanine conjugates of (‐)‐JA could be identified from extracts of the broad bean Vicia faba L.

show abstract

Biosynthesis and Metabolism of Plant Hormones

Cited by 132 publications

References 1,006 publications

Metabolism of Tryptophol in Higher and Lower Plants

Metabolism of Tryptophol in Higher and Lower Plants

Anatomical, Physiological, and Hormonal Aspects of Abscission in Citrus

Gas chromatographic/mass spectrometric and tandem mass spectrometric investigations of synthetic amino acid conjugates of jasmonic acid and endogenously occurring related compounds fromVicia faba L

Contact Info

Product

Resources

About