Biosynthesis of amylase inhibitor by Streptomycete cultures

Sharova, N. Yu.

doi:10.1134/s000368381608007x

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…The inhibitor activity decreases with increasing duration of fermentation, possibly due to its structural modification. This is acknowledged for the studied glycosidase inhibitors synthesised by streptomycetes during the fermentation of carbohydrate-containing media and being pseudo-oligosaccharides represented by the multiple forms in their chemical nature [16,17].…”

Section: рис 3 влияние температуры криогенного консервирования на дmentioning

confidence: 99%

Cryopreservation potential of Streptomyces lucensis and Streptomyces violaceus actinomycete collection strains as producers of glycosidase inhibitors

Выборнова¹,

Шарова²,

Принцева³

2019

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY

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The article presents a study into the effect of long-term, low-temperature (-80 and-150 °С) storage on the properties of Streptomyces lucensis RNCIM As-1743 and Streptomyces violaceus RNCIM As-1734 actinomycete collection strains acting as producers of glycosidase inhibitors. The titre (CFU in 1 cm 3 of the initial inoculum) and the inhibitory activity of strains were determined with respect to pancr eatic α-amylase in the solutions obtained by Streptomyces culture on a corn starch hydrolysate. For Streptomyces, a high survival rate (91-100 %) was established after storage at temperatures of-80 and-150 °C using a 15 % glycerol solution in terms of a cryoprotector. Streptomyces violaceus strain was identified to be the most resistant to long-term storage at low temperatures. Its inhibitory activity turns to be completely retained after storage at temperatures of-80 and-150 °С. In Streptomyces violaceus strain, the maximum activity level of 2250±200 IU/cm 3 for an inhibitor of pancreatic α-amylase is observed on the 1st day of subculture, while Streptomyces lucensis RNCIM As-1743 demonstrates the highest activity value on the 3rd day to reach a value of 3660±200 IU/cm 3 following storage at a temperature of-80 °С. The studied Streptomyces strains are chromogenic. The most intense chromogenesis is noted during the culture of Streptomyces violaceus strain stored at a temperature of-150 °С. The cryopreservation of Streptomyces violaceus and Streptomyces lucensis actinomycete strains was established to provide high (10 7-10 8) cell survival and preservation of their inhibitory activity at a high level when exposed to temperatures of-80 and-150 °С with a 15 % glycerol solution as a cryoprotector. Experimental data indicate the low-temperature storage method to be promising for Streptomyces lucensis and Streptomyces violaceus collection cultures.

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Section: рис 3 влияние температуры криогенного консервирования на дmentioning

confidence: 99%

Cryopreservation potential of Streptomyces lucensis and Streptomyces violaceus actinomycete collection strains as producers of glycosidase inhibitors

Выборнова¹,

Шарова²,

Принцева³

2019

PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY

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Effect of Carbohydrate Substrate from Starch-Containing Raw Materials on the Composition of Glucans in the Micromycete Aspergillus niger VKPM F-171 Biomass

Manzhieva

Принцева

Выборнова

et al. 2020

Biiotehnologiâ (Mosk.)

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The conditions for biocatalytic cleavage of corn starch and rye whole grain flour for the subsequent cultivation of the Asperillus niger strain VKPM F-171 are described. As a result of this procedure, the amount of water-soluble carbohydrates in the substrates increased by 5-6 times in comparison with the initial raw material, and the content of glucan p-form in the hydrolysate of rye whole grain flour grew by 2-3 times. The content of glucans in the biomass by the end of the cultivation of A. niger VKPM F-171 on corn starch hydrolysate was 28±2% of dry matter, while the proportion of glucans after fermentation on the rye whole grain flour hydrolysate was 21±1%, 96-97% of which were β-glucans. The hydrolysis with Trichoderma longibrachiatum β-glucanase and Trichoderma reesei xylanase resulted in a 1.1-1.2-fold increase in the proportion of soluble carbohydrates in microbial biomass. It was found via gas-liquid chromatography that the structural units of carbohydrates in microbial biomass are represented by glucose and its 2- and 6-deoxy derivatives, fructose, fucose, galactose, mannose, xylose, and, to the greatest extent, by the reserve saccharide sedoheptulose. It was established that β-glucans are products of hydrolysis of the chitin-glucan complex and glycopeptides. The molecular weight of the obtained microbial glucan-containing biopolymers ranges from 0.5 to 50 kDa. It was shown that the content of β-glucans in microbial biomass allows the latter to be considered as an alternative to plant sources. biosynthesis, Asperillus niger, biocatalysis, starch-containing raw materials, glucans

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Biosynthesis of amylase inhibitor by Streptomycete cultures

Cited by 2 publications

References 9 publications

Cryopreservation potential of Streptomyces lucensis and Streptomyces violaceus actinomycete collection strains as producers of glycosidase inhibitors

Cryopreservation potential of Streptomyces lucensis and Streptomyces violaceus actinomycete collection strains as producers of glycosidase inhibitors

Effect of Carbohydrate Substrate from Starch-Containing Raw Materials on the Composition of Glucans in the Micromycete Aspergillus niger VKPM F-171 Biomass

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