Collection cultures of actinomycetes are mainly stored in a dried state on adsorbents. Practiced low-temperature storage of actinomycetes at minus 70°C. The article presents the results of investigations of the properties of the conidia of strains of the actinomycete Streptomyces lucensis VKPM Ac–1743 and Streptomyces violaceus VKPM Ac–1734 is in the process of storage at minus 12°C and minus 18°C in glycerin solution and in 0,9 % aqueous sodium chloride solution. It was found that the inhibitory activity in the native solution for the studied strains stored in the glycerin solution at minus 12°C and plus 4°C, as a result of their subsequent cultivation on the starch hydrolyzate for 120 h is at the level of (450 ± 10) IE/cm3. The indicator for crops stored at minus 18°C was higher (560 ± 10) IE/cm3. Low-temperature storage of conidia in saline solution is less effective. Pigmentation is more active in the cultivation of strains stored at minus 18°C.

Всероссийский научно-исследовательский институт пищевых добавокфилиал Федерального научного центра пищевых систем им. В.М. Горбатова РАН, Санкт-петербург, Россия Ключевые слова: лимонная кислота, продуцент, Aspergillus niger, инвертазная активность, гидролизат, крахмал. А Н НОтА ц И Я В результате исследований установлено, что штаммы микромицета Aspergillus niger Л-4 и В-3-продуценты лимонной кислоты могут синтезировать гидролитические ферменты с инвертазной активностью при глубинном способе культивирования на сахарозоминеральной среде и среде, на основе гидролизата крахмала. Наиболее предпочтительными режимами ведения биотехнологического процесса являются: возраст посевного мицелия-24 ч, температура ферментации для сахарозоминеральной среды-32 °C и для гидролизата крахмала-29 °C. Для штамма микромицета Aspergillus niger Л-4 экстрацеллюлярная инвертазная активность на 120 ч биотехнологического процесса в результате ферментации сахарозоминеральной среды составляла (0,847 ± 0,068) ед/см 3 нативного раствора, а для штамма В-3-(0,966 ± 0,077) ед/см 3 нативного раствора соответственно. при ферментации среды, на основе гидролизата крахмала, экстрацеллюлярная инвертазная активность для штамма Л-4 составляла (1,379 ± 0,097) ед/см 3 нативного раствора, а для штамма В-3-(1,597 ± 0,144) ед/ см 3 нативного раствора соответственно. Штаммы гриба Aspergillus niger Л-4 и В-3 при культивировании на сахарозоминеральной среде и среде, на основе гидролизата крахмала, обладают способностью синтезировать ферменты с инвертазной активностью, причем инвертазная активность штамма В-3 в конце процесса выше. полученные данные могут быть применены в дальнейших исследованиях для разработки технологии получения лимонной кислоты и инвертазы в одном биотехнологическом процессе.

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Inhibitors synthesized by the Streptomyces lucensis VKPM AS-1743 and Streptomyces violaceus VKPM AS-1734 strains were studied for their influence on amylases of different origin. The effect of the inhibitors was shown to be different on fungal amylase, pancreatic amylase, and amylase from human blood. It has been found that the studied inhibitors are substances of a pseudooligosaccharide nature and exhibit their activity and stability over a wide range of pH and temperature values. The physico-chemical and biochemical properties of isolated inhibitors were compared with those of known microbial inhibitors of α-glucosidases.

The aim of the present study is to explore the transformation of (1→3)(1→4)-β-D-glucans of rye biomass byAspergills niger and accumulation of (1→3)(1→6)-β-D-glucans in the microbial cell wall.Biomass from rye grain was obtained as a result of enzymatic hydrolysis of grain grinding of Omsk region of non-standard quality with grain impurity content of 45 ± 2 % by preparations (1→4)-β-glucanolytic, (1→3)-β-glucanolytic, (1→4)-xylanolytic and (1→4)-amylolytic action. Fermentation of hydrolysates, sucrosemineral and molasses medium by A. niger was carried out by a batch process under aerobic conditions. Determined the content of β-glucans, amino-nitrogen, glucose, disaccharides in grinding grain rye, rye biomass, the biomass of A. niger, the supernatants by colorimetric methods. Determination of chitin in biomass and qualitative determination of chitosan in supernatants of hydrolysates was carried out using chitosan sulfate sample and subsequent microscopy.The results of the research showed that (1→3)(1→4)-β-D-glucans in grain grinding are 10.2 ± 0.2 % in terms of dry matter, which exceeds the content of polysaccharide in the grain of standard quality by 1.5 – 3 times. In rye biomass revealed their smaller amount, 6.4 ± 0.5 %, apparently, due to the action of (1→4)and (1→3)-β-glucanase, (1→4)-xylanase and (1→4)-amylase. In microbial mass A. niger content of (1→3)(1→6)-β-Dglucans were at the level of 21.7 ± 0.7 %.On the basis of the obtained results, it was concluded that it is possible to use rye grain of non-standard quality, with a high content of grain impurities and a low proportion of starch polysaccharides, as a source of β-glucancontaining substrate for biosynthesis (1→3)(1→6)-β-D-glucans by A. niger having advantages over (1→3) (1→4)-β-D-glucans of plant origin. They are functionally more active and have a wide range of applications, namely as food additives in the manufacture of a wide range of products: for the enrichment of fibers, increasing the shelf life of products due to its water-binding properties, as thickeners, emulsifying and fat-reducing microingredients, stabilizers of creamy emulsions, textureformers, flavor enhancers.