Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Abstract

This study was aimed at developing a simple, eco-friendly and cost effective green chemistry method for the synthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum aqueous leaf and flower extracts, respectively as the natural reducing agents. The successive reduction of chloroauric acid and silver nitrate led to the formation of Au-Ag nanoparticles within 10 min at room temperature, suggesting a higher reaction rate than chemical methods involved in the synthesis. Stable, spherical nanoparticles with well-defined dimensions of average size of 3-25 nm was confirmed by UV-Visible spectroscopy, TEM, SEM-EDX, DLS, and zeta potential, whilst, FTIR in combination with GC-MS analyzed the functional groups adhered to the surface of the nanoparticles. The colloidal suspension displayed enhanced antihyperglycemic activity at 69.97 ± 3.42% (leaf) against α-amylase (from porcine) and at 85.77 ± 5.82% (flower) against Bacillus stearothermophilus α-glucosidase than that of acarbose and their respective crude extracts. Furthermore, revealed good antibacterial activity against bacterial species Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa.

Bio-nanobactericides: an emanating class of nanoparticles towards combating multi-drug resistant pathogens

“…Hence in recent times, bimetallic nanoparticles are extensively studied as antimicrobial agents against an array of pathogenic microorganisms. According to a study conducted by Malapermal et al [73], silver-gold bimetallic nanoparticles were synthesized using aqueous leaf and flower extracts of Ocimum basilicum as a natural reducing agent. The synthesized bimetallic nanoparticles remained stable and showed significant activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa [73].…”

“…According to a study conducted by Malapermal et al [73], silver-gold bimetallic nanoparticles were synthesized using aqueous leaf and flower extracts of Ocimum basilicum as a natural reducing agent. The synthesized bimetallic nanoparticles remained stable and showed significant activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa [73]. Similarly, a study conducted by Sawle et al [74], fungal mediated bimetallic nanomaterials of silver-gold nanoparticles were synthesized possesses catalytic and antimicrobial which have a broad range of applications in health care sector.…”

Bimetallic nanoparticles as antimicrobials

“…The combined Pt and Au have a strong bactericidal effect compared with each alone nontoxic to bacteria [19] . Bimetallic Au-Ag nanoparticles was synthesised by using Ocimum basilicum aqueous leaf and flower extracts and revealed a high antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa [20] . The antibacterial efficiency of Cu-TiO 2 nanoparticles greater than either TiO 2 or Cu nanoparticles when used separately [21] .…”

BIOSYNTHESIS OF ZnO NANOPARTICLES USING OCIMUM BASILICUM AND DETERMINATION OF ITS ANTIMICROBIAL ACTIVITY

“…It is widely used in biomedicine with enormous applications like drug delivery, anti-diabetic, anti-cancer and agricultural properties (Wodka et al 2010). It has been widely used in cosmetic due to UV filtering properties especially in Sunblock lotions (Malapermal et al 2015). During this study medicinally famous herbal plant; Ocimum basilicum commonly known as Niazbo was used to prepare ZnO NPs.…”