Biosynthesis of Coumarin Glycosides by Transgenic Hairy Roots of<i>Polygonum multiflorum</i>

Zhou, Liangbin; Tian, Tian; Xue, Bailin; Song, Liyan; Liu, Ling; Yu, Rongmin

doi:10.1271/bbb.110347

Cited by 12 publications

(7 citation statements)

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“…Medicinal plants have been widely explored for hairy root culture and their secondary metabolites (Gupta et al 2011; Wilczanska-Barska et al 2012). Recently, biotransformation of coumarin glycosides by transgenic hairy roots of Polygonum multiflorum was reported using different substrates (Zhou et al 2012). …”

Section: Introductionmentioning

confidence: 99%

Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

et al. 2014

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BackgroundGentiana scabra is commonly known as ‘Longdan’ is an important herb in traditional Chinese medicines, commonly used for the treatment of inflammation, anorexia, indigestion and gastric infections. Iridoids and secoiridoids are main bioactive compounds which attributed to the pharmacological properties of this plant. The use of hairy root cultures as an excellent alternative for the production of pharmaceutically important metabolites in less time period with ensured quality of raw materials.ResultsAn efficient hairy root culture system of Gentiana scabra and influence of different plant growth regulators (PGRs) on the production of gentiopicroside, swertiamarin and loganic acid constituents were described. Leaf explants were infected with Agrobacterium rhizogenes, which induced hairy roots up to 21%. The transformed hairy root lines were confirmed by PCR using rolB and rolC gene-specific primers. Among various solid and liquid media, B5 liquid medium resulted maximum root biomass (36- fold higher) in 4-weeks. Quantitative analysis showed loganic acid was 6.6- fold higher in the presence of zeatin (1 mg/l) and gentiopicroside accumulation was 1.8- fold higher in the presence of naphthaleneacetic acid (NAA, 1 mg/l), as compared to the roots of plants grown in greenhouse. On the other hand, 1.4- and 2.5- fold higher gentiopicroside and swertiamarin were observed in the presence of 1.0 mg/l NAA as compared to commercial Gentiana herb No. 2. The result also showed iridoid and secoiridoid contents affected greatly by age, physiology and growing environment of the plant.ConclusionsThe use of hairy root cultures is an excellent alternative to harvesting natural or in vitro grown plants to produce pharmaceutically important metabolites in less time with ensured quality.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-55-17) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

et al. 2014

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“…HRs are obtained by infecting explants with Agrobacterium rhizogenes, a gram-negative soil bacterium that integrates a DNA segment into the genome of a wide variety of higher plants, including dicotyledon, gymnosperm, and some monocotyledon host species, and subsequently induces the formation of adventitious roots. Two Polygonum species (P. tinctorium and P. multiflorum) have been successfully transformed with A. rhizogenes (Chae et al 2010;Zhou et al 2012), however, to date, no study has been published on HR cultures of P. minor or the identification and quantification of its secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth

Ashraf

Zain

Zainal

et al. 2014

Plant Cell Tiss Organ Cult

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A method for hairy root induction of Persicaria minor, was developed and investigated, and a qualitative evaluation of the sesquiterpenes in the culture medium was performed. The transgenic status was confirmed by PCR analysis using rolB and virD specific primers. The efficiency with which four different strains of Agrobacterium rhizogenes (A4, ATCC43056, ATCC15834 and ATCC13333) and different concentrations of acetosyringone induced hairy roots from in vitro leaf segments of P. minor was evaluated. Strains A4 and ATCC13333 were able to induce hairy root in an average of 6 days of cocultivation and exhibited significantly higher transformation efficiencies, 20 and 21.3 %, respectively, compared to the other strains in the presence of 100 lM acetosyringone. Half-strength Murashige-Skoog basal medium supplemented with Gamborg vitamins supported the greatest increase (35-fold) of growth index of hairy roots relative to the initial weight after 8 weeks of culture. GC-MS results showed that b-caryophyllene was the main sesquiterpenes detected and was quantified with standards, with total peak areas of 7.934. Results suggested that hairy roots secreted sesquiterpenes into the medium while preserving viable hairy roots as a source of specialized metabolites.

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“…Comparison of the 1 H and 13 C NMR data with those of I revealed that compound I-1 is a glucoside of I (Table 2). 10 In the HMBC spectrum, the correlation of the anomeric proton signal at d 4.96 (H-1 0 ) to the carbon signal at d 158.0 (C-7) indicated that the sugar was linked to C-7. The 13 C NMR chemical shi of the anomeric carbon, along with the 1 H NMR chemical shi and spin-spin coupling constant (J ¼ 7.2 Hz) of the anomeric proton, allowed for the identication of a bglucopyranosyl moiety.…”

Section: Identication Of Transformed Productsmentioning

confidence: 98%

“…9 Nevertheless, coumarin glycosides could be obtained via efficient, mild, and regio-or stereoselective biotransformation of coumarins in plant cells. 10 Biotransformation catalyzed by biocatalysts, including enzymes, whole cells, or microorganisms, is widely used for biological structural modication with regio-and stereoselectivity, and this process has great potential to generate soluble and non-toxic metabolites via mild reaction conditions and fewer steps. [11][12][13][14] Several studies on the bioconversion of coumarins in plant cells have been reported.…”

Section: Introductionmentioning

confidence: 99%

“…Nine hydroxycoumarins with different substituted groups were biotransformed to investigate the substrate specicity and regioselectivity of coumarin glycosyltransferases in the hairy roots of Polygonum multiorum. 10 Zhou et al reported that kellerin, a sesquiterpene coumarin derivative, was transformed into nine novel coumarin derivatives via dehydration, dehydrogenation, hydroxylation, and reduction using the callus of Angelica sinensis (Oliv.) Diels.…”

Section: Introductionmentioning

confidence: 99%

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Biotransformation of 4-methylcoumarins by cambial meristematic cells of Camptotheca acuminata

et al. 2019

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Biosynthesis of Coumarin Glycosides by Transgenic Hairy Roots ofPolygonum multiflorum

Cited by 12 publications

References 13 publications

Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

Establishment of hairy root lines and analysis of iridoids and secoiridoids in the medicinal plant Gentiana scabra

Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth

Biotransformation of 4-methylcoumarins by cambial meristematic cells of Camptotheca acuminata

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