Biosynthesis of isocitric acid from ethanol by yeasts

Finogenova, T. V.; Shishkanova, N. V.; Fausek, E. A.; Eremina, S S

doi:10.1007/bf00164426

Cited by 33 publications

(30 citation statements)

References 6 publications

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“…The factors above determined the excretion of acetic acid, CA, and ICA. Completion of this series of studies [45][46][47] resulted in the development of a reliable technology for obtaining ICA. Large batches of monosodium isocitrate, ordered by the companies Biotics (Moscow) and Biopharm (Tomsk), were produced using a pilot technological plant (PTP) set up at IBPM.…”

Section: Regulation Of Biosynthesis Of Citric and Isocitric Acids By mentioning

confidence: 99%

Organic Acid Production by the Yeast Yarrowia lipolytica: A Review of Prospects

Finogenova

Morgunov

Kamzolova

et al. 2005

Appl Biochem Microbiol

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164

108

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The review sums up the results of studies of (1) physiological growth characteristics of the yeast Yarrowia lipolytica cultured in the presence of diverse carbon sources ( n -alkanes, glucose, and glycerol) and(2) superhigh synthesis of organic acids, which was performed at the Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences. Microbiological processes of obtaining α -ketoglutaric, pyruvic, isocitric, and citric acids are discussed. In memoriam Aleksandr Borisovich Lozinov

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Section: Regulation Of Biosynthesis Of Citric and Isocitric Acids By mentioning

confidence: 99%

Organic Acid Production by the Yeast Yarrowia lipolytica: A Review of Prospects

Finogenova

Morgunov

Kamzolova

et al. 2005

Appl Biochem Microbiol

Self Cite

164

108

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“…Just which reaction is the most sensitive to high concentrations of ethanol needs to be specially studied. Some authorities report the appearance of acetaldehyde and acetic acid in the medium during yeast culturing in ethanol [2,5]. These metabolites may be potential inhibitors of many cell functions.…”

Section: Resultsmentioning

confidence: 99%

“…The composition of the biomass and components of the culture fluid were analyzed as described previously [5].…”

Section: Methodsmentioning

confidence: 99%

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Effect of ethanol concentration on the maximal specific growth rate and biomass composition ofYarrowia lipolytica mutant strain No. 1

et al. 1996

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The effect of ethanol concentration on the maximal specific growth rate and biomass composition of Yarrowia lipolytica No. 1 was studied during culturing in the pH-auxostat mode. Growth inhibition set in starting from a 2.64 g/liter residual concentration of ethanol. The constant of ethanol inhibition was 11.0 g/liter. Growth inhibition with ethanol was associated with changes of the fatty-acid composition of lipids and a resultant reduction of lipid unsaturation.

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“…It was recently reported that conditions for ICA overproduction by Yarrowia lipolytica consist of limit ing yeast growth by restricting the mineral compo nents in the medium (nitrogen, phosphorus, sulfur or magnesium) while providing access to a carbon source and thiamine [8][9][10]. Vegetable oils are promising substrates for the production of a number of sub stances using microorganisms [2,4].…”

mentioning

confidence: 99%

Biosynthesis of isocitric acid by the yeast Yarrowia lipolytica and its regulation

Kamzolova

Lunina

Allayarov

et al. 2015

Appl Biochem Microbiol

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We studied the biosynthesis of isocitric acid from rapeseed (canola) oil by the yeast Yarrowia lipolytica and its regulation. We determined a fundamental possibility for directed biosynthesis of isocitric acid by Y. lipolytica yeast, with only minimal amounts of citric acid byproduct, when grown on a medium containing canola oil. Wild type strains of Y. lipolytica were mutagenized by UV irradiation and treatment with N methyl N' nitro N nitrosoguanidine (NG). Subsequent selection on media with acetate and isoci trate resulted in isolation of a mutant Y. lipolytica UV/NG that synthesized isocitrate and citrate at a ratio of 2.7 : 1. In the parent strain, this ratio is 1 : 1. Inhibition of isocitrate lyase, a key enzyme in the metabolism of isocitric acid, by the addition of itaconic acid resulted in increased synthesis of isocitrate, with a ratio of isoci trate to citrate reaching 6 : 1. The cultivation of the Y. lipolytica UV/NG mutant in a pilot industrial fermenter in the presence of itaconic acid resulted in the production of 88.7 g/L of isocitric acid with a yield of 90%.

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Biosynthesis of isocitric acid from ethanol by yeasts

Cited by 33 publications

References 6 publications

Organic Acid Production by the Yeast Yarrowia lipolytica: A Review of Prospects

Organic Acid Production by the Yeast Yarrowia lipolytica: A Review of Prospects

Effect of ethanol concentration on the maximal specific growth rate and biomass composition ofYarrowia lipolytica mutant strain No. 1

Biosynthesis of isocitric acid by the yeast Yarrowia lipolytica and its regulation

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