Biosynthesis of the pyoverdine siderophore of <i>Pseudomonas aeruginosa</i> involves precursors with a myristic or a myristoleic acid chain

Hannauer, Mélissa; Schäfer, Matthias; Hoegy, Françoise; Gizzi, Patrick; Wehrung, Patrick; Mislin, Gaëtan L. A.; Budzikiewicz, H.; Schalk, Isabelle J.

doi:10.1016/j.febslet.2011.12.004

Cited by 59 publications

(103 citation statements)

References 27 publications

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“…The molecular weight of purified pyoverdine was about 1278 (Fig. S7), which was similar with the previous researches [24,25]. The little difference was resulted from the variable peptide chain of pyoverdine, which could also be utilized as a fingerprint method to distinguish strains from each other [26].…”

Section: Pyoverdine Purificationsupporting

confidence: 86%

A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

Yin

Wang

et al. 2016

Sensors and Actuators B: Chemical

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a b s t r a c tThe environmental copper pollution seriously threatens the health of organisms and the safety of ecosystem. Therefore, the development of a simple and sensitive method to detect copper ion is very important. In this study, we have developed a fluorescent biosensor based on biological recognition element pyoverdine to selectively detect copper ion. The fluorescence of pyoverdine is quenched obviously after binding with copper ion. A good linearity within the range of 0.2-10 M (R = 0.997) is attained and the detection limit is 50 nM. The biosensor has been successfully utilized for the detection of copper ion in drinking water, seawater and bio-samples and the results agree well with those obtained by the inductively coupled plasma mass spectrometry. Therefore, the established biosensor is a creditable method to detect copper ion with high sensitivity and selectivity, which can be utilized as a powerful tool to monitor copper pollution in the environment.

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Section: Pyoverdine Purificationsupporting

confidence: 86%

A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

Yin

Wang

et al. 2016

Sensors and Actuators B: Chemical

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“…The mobile phase was acetonitrile to NaOH-acetic acid (17 mM, pH 5.3), with acetonitrile gradient ranged from 0% to 100% over 40 min at a flow rate of 1.0 ml/min. The detection was monitored at 410 nm under an UV detector (Hannauer et al, 2012).…”

Section: Purification Of Pyoverdinementioning

confidence: 99%

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Yin

Zhang

Chen

2014

Biosensors and Bioelectronics

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a b s t r a c tMethods for the rapid and sensitive detection of furazolidone, a pesticide used for the treatment of infections of animals and human beings, have been urgently recommended for its large residual, strong carcinogenicity and genotoxicity in the environment. In this study, a method for the detection of furazolidone based on the rapid fluorescence quenching of pyoverdine by furazolidone was developed. Pyoverdine secreted by a Pseudomonas aeruginosa strain PA1 was purified through affinity chromatography and its fluorescent property was characterized. The fluorescence of pyoverdine could be quenched by furazolidone with specificity, and based on this phenomenon a fluorescent method for furazolidone detection was established. Fluorescence of pyoverdine was quenched by furazolidone probably due to the electron transfer from pyoverdine to furazolidone. The optimal pH for the detection was 7.2 in 50 mM 3-(N-Morpholino) propanesulfonic acid solution, and the whole detection process could be completed within a few seconds. The linear range of the detection was 2-160 mM and the limit of detection (LOD) was 0.5 mM. This study developed a novel fluorescent method for furazolidone detection, and the rapid and specific fluorescent biosensor can be potentially applied for furazolidone detection in the aquatic samples.

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“…The assigned structures are illustrated in Figure 4BD. While the wild type strain produced the well-known pyoverdines PVD-α-ketoglutaric acid [2], PVDsuccinamide [3], and PVD-succinic acid [4], the data clearly showed that the pyoverdines of the pvdO deletion mutant were the known dihydrointermediates of the pyoverdines with the different modifications known for the first residue, namely glutamic acid [5], α-ketoglutaric acid [6], succinamide [7], and succinic acid [8]. The succinic acid form [8] was very low abundant, and some ferribactin [9] was detected in both preparations, especially in the pvdO deletion strain.…”

Section: Resultsmentioning

confidence: 99%

“…Fluorescent pseudomonads warrant their iron supply by pyoverdines, fluorescent siderophores that are derived from non-ribosomally synthesized peptides that are called ferribactins (2) (Figure 1). Ferribactins are transported into the periplasm in acylated form and deacylated thereafter by PvdQ (3)(4)(5). In the periplasm, a 5,6-dihydroxyquinoline fluorophore is generated from ferribactin and the conserved glutamic acid residue at the first position of the peptide is modified (6)(7)(8).…”

Section: Discussionmentioning

confidence: 99%

PvdO is required for the oxidation of dihydropyoverdine as the last step of fluorophore formation in Pseudomonas fluorescens

Ringel

Dräger

Brüser

2018

Journal of Biological Chemistry

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SUMMARYPyoverdines are important siderophores that guarantee iron supply to important pathogenic and non-pathogenic pseudomonads in host habitats. A key characteristic of all pyoverdines is the fluorescent dihydroxyquinoline group that contributes two ligands to the iron complexes. Pyoverdines are derived from the non-ribosomally synthesized peptide ferribactin and their fluorophore is generated by periplasmic oxidation and cyclization reactions of D-tyrosine and Ldiaminobutyric acid. The formation of the fluorophore is known to be driven by the periplasmic tyrosinase PvdP. Here we report that the putative periplasmic oxidoreductase PvdO of Pseudomonas fluorescens A506 is required for the final oxidation of dihydropyoverdine to pyoverdine, which completes the fluorophore. The pvdO deletion mutant accumulates dihydropyoverdine, and this phenotype is fully complemented by recombinant PvdO. The autoxidation of dihydropyoverdine at alkaline pH and the presence of high copper concentrations can mask this phenotype. Mutagenesis of conserved residues with potential catalytic function identified Glu-260 as essential residue whose mutation abolished function without affecting stability or transport. Glu-260 of PvdO is at the exact position of the active site cysteine in the structurally related formylglycine-generating enzyme. Evolution thus used the same protein fold for two distinct functionalities. As purified PvdO was inactive, additional factors are required for catalysis.

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Biosynthesis of the pyoverdine siderophore of Pseudomonas aeruginosa involves precursors with a myristic or a myristoleic acid chain

Cited by 59 publications

References 27 publications

A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

PvdO is required for the oxidation of dihydropyoverdine as the last step of fluorophore formation in Pseudomonas fluorescens

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