Biotechnological and Medical Aspects of Lactic Acid Bacteria Used for Plant Protection: A Comprehensive Review

Bergsma, Simon; Euverink, Gert-Jan; Charalampogiannis, Nikolaos; Poulios, Efthymios; Janssens, Thierry; Achinas, Spyridon

doi:10.3390/biotech11030040

Cited by 4 publications

(4 citation statements)

References 122 publications

(113 reference statements)

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“…Unreasonable doses of mineral fertilizers, the use of chemical means to control plant diseases and the failure to comply with the technology of their application bring harm to the environment (Raman et al, 2022). Thus, scientists search for ecological methods of protection from the agents of crop diseases, which could be found in microbes-antagonists for the elaboration of biopreparations on their basis (Dragovoz et al, 2014;Hrabova et al, 2015;Davranov et al, 2019;Bergsma et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…However, the increase in their application impacts the ecological equilibrium and safety of food products and is the main factor of environmental pollution (Ra man et al, 2022). Th erefore, as an alternative to chemical preparations, researchers pay great attention to the elaboration of ecologic methods of protection against crop disease agents, using microbial preparations, based on different species of microorganisms and their metabolites (Bergsma et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Bacteria-antagonists of the agents of soryz bacterial diseases

Reshetnikov,

Patyka

2024

Agric. sci. pract.

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Aim. To isolate and identify bacteria with antagonist properties for biocontrol of the agents of bacterial diseases of soryz (Sorghum oryzoidum) and sorghum crops. Methods. The studies were conducted in 2021-2023. Spore-forming bacteria were isolated from the soryz samples, collected in the fields of the experimental farm of the Uman National Horticulture University (Cherkasy region, Uman). Lactic acid bacteria were isolated from soryz plants, collected in the private land plot, located between the villages of Teolyn, Vladyslavchyk, Kniazhyky in Monastyryshche com- munity, Uman district, where Pershotravneve hamlet used to be situated. A total of 1,250 samples were analyzed. The experiment had three repeats. Spore-forming and lactic acid bacteria were isolated from the surface of soryz plants on the firm ripe stage in summer while isolating phytopathogenic bacteria. The isolates of lactic acid bacteria- antagonists were also isolated from the inner part of winter stubble stalk of soryz, collected from the tilled field. The antagonistic activity of the strains of lactic acid bacteria and spore-forming bacteria, isolated from different ecological niches, to phytopathogens of soryz and sorghum crops was determined in vitro. The strains of Pseudomonas syringae, the agents of soryz bacterial spots, were used as test-cultures: 211141a, 211141, 210341, 21034, and 210521, along with the collection strains of phytopathogens: Pseudomonas syringae 8299, Pseudomonas syringae subsp. syringae UKM B-1021, X. oryzae 8375, Dickeya chrysanthemi 8683, Diskeya chryzanthemi 8683. The antagonistic activity of the extracted isolates of spore-forming and lactic acid bacteria was studied using the method of radial strokes (joint cultivation of the antagonist and the strains under investigation). The bacterial isolates were deemed inactive if the growth delay zone was 0–5 mm (–), from 5 to 10 mm (+) – low activity, 11–20 mm (++) – moderate activity, over 20 mm (+++) – high activity regarding the test-cultures. To check the effect of the isolate-antagonist of phytopathogenic bacteria, artificial infecting was conducted in the field conditions. For this purpose, a diurnal culture of the antagonist was introduced into the stalk of plants in the concentration of 1×108 colony-forming units, and 24 h later, a culture of test-strain of the phytopathogen was administered above the previous puncture. The results were evaluated 7–14 days after the artificial infection. The experiment had three repeats. The isolates of bacteria which demonstrated their an- tagonistic properties regarding the phytopathogenic bacteria were identified by their morphological properties, Gram staining, catalase test, profile of carbohydrate fermentation and mass-spectrometry (MALDI-TOF – Matrix Assisted Laser Desorption/Ionization) using VITEK MS mass-spectrometer. Results. Thirty-eight spore-forming bacterial iso- lates were extracted from soryz; among these, 21030, 21095, 21040, ASV1, ASV3, B4 demonstrated their antagonistic activity towards the investigated phytopathogenic bacteria. Isolate 21040 showed high antagonistic activity to most test-strains of P. syringae from soryz (the zone of negative culture – 23–30 mm) and lower activity regarding the collection cultures. Isolates B4 and AVS3 demonstrated their selective activity regarding the investigated phytopatho- gens. Twenty isolates of lactic acid bacteria were extracted. Higher antagonistic activity was noted for the isolates of lactic acid bacteria 8/1 and F1 to the strains of P. syringae, isolated from soryz and collection cultures. The highest antagonistic activity of isolate 8/1 was noted regarding test-strains of P. syringae 210521 and X. oryzae 8375 (the zone of negative culture – 40–35 mm). In the field conditions, the treatment of sorghum plants with F1 affected the pathological process that developed due to the impact of the phytopathogenic bacteria P. syringae, which led to the reduction in disease symptoms. The taxonomic position of the isolates of bacteria, which seem to be promising for the control of disease agents, was determined. In terms of morphology of cells and colonies, the biochemical profile, and mass-spectrometry MALDI-TOF, the spore-forming isolates 21040 and B4 were identified as Bacillus subtilis, and ASV3 – as Bacillus vallismortis. The identified isolates of lactic acid bacteria were Lactobacillus pentosus F1 and Lactobacillus sakei 8/1. Conclusions. In addition to phytopathogenic bacteria, from soryz plants we isolated the strains of spore-forming bacteria Bacillus subtilis 21040, B4, Bacillus vallismortis AVS3 and such lactic acid bacteria as Lactiplantibacillus pentosus and Lactobacillus sakei 8/1 (Latilactobacillus sakei 8/1), promising for the elaboration of methods for the biocontrol of the agents of bacterial diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacteria-antagonists of the agents of soryz bacterial diseases

Reshetnikov,

Patyka

2024

Agric. sci. pract.

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“… Arena et al (2018) claimed that a protonated form of lactic acid inhibits the pH gradient between the outside acidic environment and the inside alkaline cytosol, dissipating the membrane potential and killing cells. LAB metabolites reported that molecules such as hydrogen peroxide, acetate, butyrate, formic acid, succinate, propionate, valerate, caproic acid, and cyclic dipeptides have potent antifungal properties ( Fazeli et al, 2004 ; Bergsma et al, 2022 ; Zapaśnik et al, 2022 ). An experiment on sourdough bread between six strains of Lactiplantibacillus plantarum showed that strain AR524 at 10 8 CFU ml –1 could remove the highest amount of DON (0.359 mg kg –1 ), and this process majorly occurred in the fermentation stage ( Cao et al, 2021 ).…”

Section: Biological Control Of Fusaria By Lactic Acid Bacteriamentioning

confidence: 99%

Fusarium biocontrol: antagonism and mycotoxin elimination by lactic acid bacteria

Krishnan,

Nampoothiri,

Suresh

et al. 2024

Front. Microbiol.

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Mycotoxins produced by Fusarium species are secondary metabolites with low molecular weight formed by filamentous fungi generally resistant to different environmental factors and, therefore, undergo slow degradation. Contamination by Fusarium mycotoxins in cereals and millets is the foremost quality challenge the food and feed industry faces across the globe. Several types of chemical preservatives are employed in the mitigation process of these mycotoxins, and they help in long-term storage; however, chemical preservatives can be used only to some extent, so the complete elimination of toxins from foods is still a herculean task. The growing demand for green-labeled food drives to evade the use of chemicals in the production processes is getting much demand. Thus, the biocontrol of food toxins is important in the developing food sector. Fusarium mycotoxins are world-spread contaminants naturally occurring in commodities, food, and feed. The major mycotoxins Fusarium species produce are deoxynivalenol, fumonisins, zearalenone, and T2/HT2 toxins. Lactic acid bacteria (LAB), generally regarded as safe (GRAS), is a well-explored bacterial community in food preparations and preservation for ages. Recent research suggests that LAB are the best choice for extenuating Fusarium mycotoxins. Apart from Fusarium mycotoxins, this review focuses on the latest studies on the mechanisms of how LAB effectively detoxify and remove these mycotoxins through their various bioactive molecules and background information of these molecules.

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“…The antifungal activity of LAB results from metabolites regulated by gene expression and is considered an intraspecific trait [17]. Short-chain organic acids, bacteriocins, chitinolytic enzymes, and volatile compounds are believed to be the metabolites that inhibit fungal growth and spore germination [15,18]. Lactiplantibacillus plantarum and other lactobacilli are the most intensively studied genera of LAB in relation to their antifungal activity against Fusarium spp.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against Fusarium spp.

Kavková,

Bazalová,

Cihlář

et al. 2023

Agronomy

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Legumes are indispensable crops in sustainable agricultural systems because of their capability for biological nitrogen fixation owing to symbiosis with rhizobia and soil fertility restoration. Fungal pathogens from the genera Fusarium cause rotting and wilting and produce mycotoxins in plant tissues. The use of fungicides in sustainable agricultural systems is limited; therefore, the application of biological agents with antifungal activity against Fusarium spp. is desirable. Lactic acid bacteria (LAB) are promising control agents that produce a wide spectrum of functional metabolites. Lactiplantibacillus plantarum and other lactobacilli are the most intensively studied genera of LAB in relation to antifungal activity against Fusarium spp. However, LAB strains belonging to the lactobacilli and lactococci genera have not yet been isolated and characterised from legumes. Therefore, we aimed to obtain wild strains of LAB from legumes, screen them for functional characteristics with respect to their antifungal activity, and compare their antifungal activity against isolates of Fusarium spp. from legumes. Consequently, 31 LAB isolates belonging to 10 species were obtained and identified from legumes. Their functional properties, including genetics and proteomics, short-chain organic acid production, and antifungal activity against five Fusarium spp., of Lactiplantibacillus plantarum, Lacticaseibacillus paracasei, and Lactiplantibacillus pentosus isolates, were studied. Cell-free supernatants of L. plantarum and L. pentosus showed significant suppression of mycelial growth and conidial germination.

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Biotechnological and Medical Aspects of Lactic Acid Bacteria Used for Plant Protection: A Comprehensive Review

Cited by 4 publications

References 122 publications

Bacteria-antagonists of the agents of soryz bacterial diseases

Bacteria-antagonists of the agents of soryz bacterial diseases

Fusarium biocontrol: antagonism and mycotoxin elimination by lactic acid bacteria

Characterisation of Wild Strains of Lactic Acid Bacteria Isolated from Legumes and Their Biocontrol Potential against Fusarium spp.

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