Biotechnological approaches toward nanoparticle biofunctionalization

Avvakumova, Svetlana; Colombo, Miriam; Tortora, Paolo; Prosperi, Davide

doi:10.1016/j.tibtech.2013.09.006

Cited by 110 publications

(84 citation statements)

References 85 publications

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“…In general, non-covalent methods (electrostatic adsorption or hydrophobic interactions) are reported as simple and effective approaches for drugs bioconjugation, in the presence of functionalized AuNPs. Thanks to the relatively weak affinity of the gold surface and/or the presence of thiol stabilizing layer, several drugs can be adsorbed, with a general retention of the therapeutic activities [62][63][64].…”

Section: Resultsmentioning

confidence: 99%

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Bessar

Venditti

Benassi

et al. 2016

Colloids and Surfaces B: Biointerfaces

119

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a b s t r a c tGold nanoparticles (AuNPs) represent an effective choice for topical drug delivery systems thanks to their small size, general non-toxicity, ease of functionalization and high surface to volume ratio. Even if systemic, methotrexate still plays an important role in psoriasis treatment: its topical use shows insufficient percutaneus penetration owing to limited passive diffusion, high molecular weight and dissociation at physiological pH. The aim of our study was to design a new drug delivery nanocarrier for Methotrexate and to improve its solubility, stability and biodistribution. AuNPs were on purpose prepared with a hydrophilic stabilizing layer, in order to improve the colloidal stability in water. Watersoluble gold nanoparticles functionalized by sodium 3-mercapto-1-propansulfonate (Au-3MPS) were prepared and loaded with methotrexate (MTX). The loading efficiency of MTX on Au-3MPS was assessed in the range 70-80%, with a fast release (80% in one hour). The release was studied up to 24 h reaching the value of 95%. The Au-3MPS@MTX conjugate was fully characterized by spectroscopic techniques (UV-vis, FTIR) and DLS. Preliminary toxicity tests in the presence of keratinocytes monolayers allowed to assess that the used Au-3MPS are not toxic. The conjugate was then topically used on C57BL/6 mouse normal skin in order to trace the absorption behavior. STEM images clearly revealed the distribution of gold nanoparticles inside the cells. In vitro studies showed that Methotrexate conjugated with Au-3MPS is much more efficient than Methotrexate alone. Moreover, DL50, based on MTT analysis, is 20 folds reduced at 48 h, by the presence of nanoparticles conjugation. UV-vis spectra for in vivo tracing of the conjugate on bare mouse skin after 24 h of application, show increased delivery of Methotrexate in the epidermis and dermis using Au-3MPS@MTX conjugate, compared to MTX alone. Moreover we observed absence of the Au-3MPS in the dermis and in the epidermis, suggesting that these layers of the skin do not retain the nanoparticles. Based on our data, we found that the novel Au-3MPS@MTX conjugate is an effective non-toxic carrier for the satisfactory percutaneous absorption of Methotrexate and could help in possible topical treatment of psoriasis.

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Section: Resultsmentioning

confidence: 99%

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Bessar

Venditti

Benassi

et al. 2016

Colloids and Surfaces B: Biointerfaces

119

View full text Add to dashboard Cite

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“…The economy of biocatalytic process can be improved by enzyme reusability and stability by means of immobilization [2,3]. The challenges of using immobilized enzymes are identifying new matrix materials with appropriate structural characteristics, such as morphology and surface functionality [4].…”

Section: Introductionmentioning

confidence: 99%

Laccase-conjugated amino-functionalized nanosilica for efficient degradation of Reactive Violet 1 dye

Gahlout

Rudakiya

Gupte³

2017

Int Nano Lett

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Immobilization of enzyme with nanostructures enhances its ideal characteristics, which may allow the enzyme to become more stable and resistant. The present investigation deals with the formulation of laccase nanosilica conjugates to overcome the problems associated with its stability and reusability. Synthesized nanosilica and laccase nanoparticles were spherical shaped, with the mean size of 220 and 615 nm, respectively. Laccase nanoparticles had an optimum temperature of 55°C and pH 4.0 for the oxidation of ABTS. Laccase nanoparticle retained 79% of residual activity till 20th cycle. It also showed 91% of its initial activity at lower temperatures even after 60 days. Laccase nanoparticles were applied for Reactive Violet 1 degradation wherein 96.76% of decolourization was obtained at pH 5.0 and 30°C within 12 h. Toxicity studies on microbes and plants suggested that the degraded metabolites were less toxic than control dye. Thus, the method applied for immobilization increased storage stability and reusability of laccase, and therefore, it can be utilized for efficient degradation of azo dyes.

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“…The role played by nanoparticles could be passively achieved by particles themselves or be actively achieved by functionalization (e.g., surface modification) [14,36,259,280,377].…”

Section: Functional Fillermentioning

confidence: 99%

Application of Nanoparticles in Manufacturing

Hu¹,

Tuck²,

Wildman³

et al. 2015

Handbook of Nanoparticles

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With the development of nanoscience and nanotechnology, manufacturing is undergoing revolutionary changes. Nanoparticles, due to their novel and often enhanced properties, are now more and more used in manufacturing. In this chapter, the state-of-the-art application of nanoparticles in manufacturing is reviewed. The contents include five parts: (a) role of nanoparticles in manufacturing, (b) manufacturing methods, (c) applications, (d) challenges, and (e) conclusions. The roles of nanoparticles are divided into three categories: (i) building blocks, being the major component of a manufactured product by solid-state or colloidal nanoparticle consolidation; (ii) functional filler, as an addition for functionality, such as property improver, catalyst, stimuli-responsive, sensing, imaging, and carrier; and (iii) nanocomposites for multifunctionality. Various manufacturing methods and novel trends are summarized in this chapter, including top-down and bottom-up, from 2D to 3D, from cleanroom to desktop, 3D printing, and bio-assisted methods, such as DNA origami. Direct applications in areas of flexible electronics, molecular electronics, solar cells, construction industry, water treatment, and biomedical devices are presented. The associated challenges including nanoparticle safety issue, sustainable manufacturing, economic issue, and scale-up are discussed. Role of Nanoparticles in ManufacturingWith the development of nanoscience and nanotechnology, manufacturing is undergoing revolutionary changes. Nanoparticles, due to their novel and often enhanced properties, have more and more been used in various manufacturing applications. Their role can generally be divided into three categories: (a) building blocks, (b) functional filler, and (c) nanocomposites for multifunctionality, which is summarized in Table 1. Building BlockNanoparticles sometimes act like the bricks in a house-building process to be the main component of a manufactured product. They can be consolidated via solid-and liquid-based methods. Solid-State Nanoparticle ConsolidationSolid-state metallic, ceramic, amorphous, and compound particles can be thermomechanically processed to form bulk 2D/3D structures with desired strength and level of densification (even full densification is possible). Reported methods include powder compact forging/extrusion, equal channel angular extrusion/ pressing, and hot pressing/sinter forging combined with conventional heating, laser sintering, microwave sintering, electric discharge sintering, or spark plasma sintering [33,255,278,306,425,426,476,492]. Particles with a narrow size distribution and spherical shape are desired to achieve low pore-to-

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Biotechnological approaches toward nanoparticle biofunctionalization

Cited by 110 publications

References 85 publications

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Laccase-conjugated amino-functionalized nanosilica for efficient degradation of Reactive Violet 1 dye

Application of Nanoparticles in Manufacturing

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