Biotechnological methods of preservation of the grape gene pool in the in vitro collection

Abstract

The article shows the results of the improvement of meristem survival, its growth characteristics, shoot formation with the combined usage of apical meristems and the preparations such as Ribavirin, salicylic acid, Melafen, the antibiotic Cefotaxime. The research results demonstrate a decrease in the intensity of growth with the presence of sucrose, fructose, and sorbitol in the culture medium. The clarification of the parameters of the preparations application made it possible to increase the duration of nonstop storage of plants up to 8–10 months.

Preserving grapevine variety Fioletoviy Ranniy in the collection in vitro

“…One way to reduce the costs is to store in vitro under conditions of slow growth. Maintenance of plant organs or whole plants in conditions that slow down the vigor reduces labor costs and the frequency of replantings, which may be accompanied by the risk of infection and stressful conditions that threat to genetic stability [17].…”