Bis-GMA affects craniofacial development in zebrafish embryos ( Danio rerio )

Kramer, Alexander G.; Vuthiganon, Jompobe; Lassiter, Christopher S.

doi:10.1016/j.etap.2016.02.018

Cited by 21 publications

(11 citation statements)

References 40 publications

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“…Twenty three resin‐matrix composites (13%) were free of BPA once urethane dimethacrylate (UDMA) or UDMA/triethylene glycol dimethacrylate (TEGDMA) replaced Bis‐GMA 3 ; On in vivo assessment including 22 human participants, the maximum concentration of BPA in saliva was around 2.0889 × 10 −2 μg/ml after placement lingual orthodontic retainers. BPA levels detected in urine did not appear to have any link to the placement of the lingual orthodontic retainers 2 ; The exposure of different content of Bis‐GMA to zebrafish embryos resulted in mortality of 55% embryos at 10 μM Bis‐GMA and 30% embryos at 1 μM Bis‐GMA 26 ; In vitro analysis detected significant amounts of BPA, TEGDMA, and other methacrylate‐based monomers released from orthodontic adhesives used in daily clinical practice. The content of BPA at around 12.54% was significantly high for inducing localized or systemic toxicity regarding the previous studies 4 …”

Section: Resultsmentioning

confidence: 97%

“…Zebrafish adults were maintained on a 14/10 h light/dark schedule. After fertilization (hpf), different content of Bis‐GMA (10 nM, 100 nM, 1 μM and 10 μM) were added for 12 h post fertilization (hpf) in the surrounding culture environment for 12 hr and their toxicity and mortality was daily evaluated 26 . The concentrations of Bis‐GMA were chosen from previous studies on the release of BPA 51 .…”

Section: Discussionmentioning

confidence: 99%

“…The concentrations of Bis‐GMA were chosen from previous studies on the release of BPA 51 . An increase in zebrafish mortality of approximately 30% was recorded at 1 μM Bis‐GMA that increased up to 55% at 10 μM Bis‐GMA 26 . The mortality was significant low (as the baseline) when the zebrafish was exposed to 10 or 100 nM.…”

Section: Discussionmentioning

confidence: 99%

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An integrative review on the toxicity of Bisphenol A (BPA) released from resin composites used in dentistry

et al. 2021

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The main aim of this study was to perform an integrative review on the release of bisphenol A (BPA) from resin‐matrix composites and potential toxic effects. A bibliographic search was performed on the PubMed platform using the following keywords: “Bisphenol A" OR “BPA” AND “resin composite” OR “composite resin” AND “toxicity” OR “cytotoxicity” OR “release”. Inclusion criteria involved in vitro and in vivo studies on the release and toxicity of BPA. Results highlighted the release of BPA from resin‐matrix composites due to insufficient polymerization and/or degradation of the polymeric matrix. BPA is part of the organic matrix of resin‐matrix composites and may be hydrolysed in human saliva, although studies report that low doses might not be detected by traditional chemical analysis. Studies exposing zebrafish embryos to different concentrations of Bis‐GMA, showed 55% mortality at 10 μM Bis‐GMA while 30% mortality was recorded at 1 μM Bis‐GMA. In patients, a BPA concentration of around 2.09 × 10−2 μg/ml was found in the saliva after placement of lingual orthodontic retainers with resin‐matrix composites. Also, the BPA molecule can be swallowed and absorbed by the oral/gastrointestinal mucosa, which might result in systemic toxicity. The degradation of resin‐matrix composites and release of BPA in oral environment are dependent on the organic matrix content and on the polymerization method. A increased release of BPA can lead to the absorption into oral and gastrointestinal mucosa with high risks of local and systemic toxicity.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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An integrative review on the toxicity of Bisphenol A (BPA) released from resin composites used in dentistry

et al. 2021

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“…Previous studies have suggested that endocrine-disrupting compounds can affect heart rate (Santos et al, 2014), bone development (Kopras et al, 2014), and cartilage formation (Kramer et al, 2016). Although atrazine does not directly bind to estrogen receptors (Roberge et al, 2004), it has been shown to elevate levels of estrogen in humans by increasing amounts of the enzyme aromatase (Sanderson et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Atrazine affects craniofacial chondrogenesis and axial skeleton mineralization in zebrafish (Danio rerio)

2018

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Atrazine is a commonly used herbicide that has previously been implicated as an endocrine-disrupting compound. Previous studies have shown that estrogenic endocrine-disrupting compounds affect the development of the heart, cartilage, and bone in zebrafish ( Danio rerio). To determine whether atrazine has effects similar to other endocrine disruptors, zebrafish embryos were treated with a range of atrazine concentrations. Atrazine treatment at a low concentration of 0.1 µM resulted in significant differences in craniofacial cartilage elements, while concentrations ≥1 µM led to decreased survival and increased heart rates. Fish treated with ≥1 µM atrazine also developed with delayed vertebrae mineralization. Higher concentrations of atrazine caused gross craniofacial defects and decreased hatching rates. Further studies into the molecular pathways disrupted in these developmental processes could shed light on a link between endocrine-disrupting compounds and developmental abnormalities.

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“…In zebrafish larvae, BPA induced craniofacial malformations by disrupting chondrocyte organization in the pharyngeal structures and inducing apoptosis (Huang et al, 2020). The BPA-based polymer Bis-GMA used in dental fillers decreased palatoquadral length in zebrafish larvae at 10 nM, while the palatoquadral-ceratohyal angle was decreased at 1 µM (Kramer et al, 2016). It was hypothesized that these effects are caused by ER-mediated altered expression of SHH and BMP pathway genes.…”

Section: Estrogens and Craniofacial Malformationsmentioning

confidence: 99%

Zebrafish Models of Craniofacial Malformations: Interactions of Environmental Factors

Raterman

Metz

Wagener

et al. 2020

Front. Cell Dev. Biol.

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The zebrafish is an appealing model organism for investigating the genetic (G) and environmental (E) factors, as well as their interactions (GxE), which contribute to craniofacial malformations. Here, we review zebrafish studies on environmental factors involved in the etiology of craniofacial malformations in humans including maternal smoking, alcohol consumption, nutrition and drug use. As an example, we focus on the (cleft) palate, for which the zebrafish ethmoid plate is a good model. This review highlights the importance of investigating ExE interactions and discusses the variable effects of exposure to environmental factors on craniofacial development depending on dosage, exposure time and developmental stage. Zebrafish also promise to be a good tool to study novel craniofacial teratogens and toxin mixtures. Lastly, we discuss the handful of studies on gene–alcohol interactions using mutant sensitivity screens and reverse genetic techniques. We expect that studies addressing complex interactions (ExE and GxE) in craniofacial malformations will increase in the coming years. These are likely to uncover currently unknown mechanisms with implications for the prevention of craniofacial malformations. The zebrafish appears to be an excellent complementary model with high translational value to study these complex interactions.

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Bis-GMA affects craniofacial development in zebrafish embryos ( Danio rerio )

Cited by 21 publications

References 40 publications

An integrative review on the toxicity of Bisphenol A (BPA) released from resin composites used in dentistry

An integrative review on the toxicity of Bisphenol A (BPA) released from resin composites used in dentistry

Atrazine affects craniofacial chondrogenesis and axial skeleton mineralization in zebrafish (Danio rerio)

Zebrafish Models of Craniofacial Malformations: Interactions of Environmental Factors

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