Bisbenzylisoquinoline alkaloids from<i>Cocculus pendulus</i>

Atta-ur-Rahman, _; Wahab, Atia‐tul; Sultani, S. Zia; Nawaz, Syed Junaid; Choudhary, M. Iqbal

doi:10.1080/14786410500185303

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…The filtrate was collected and then evaporated with a rotary evaporator (Stuart, Stone, UK) until obtained viscous extract [19], [20], [21]. The viscous extract was fractionated with n-hexane and continue with chloroform at pH 3, 7, 9 and 11 [22].…”

Section: Extraction and Fractionationmentioning

confidence: 99%

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Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits

Syari¹,

Rosidah²,

Hasibuan³

et al. 2019

Open Access Maced J Med Sci

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AIM: This study was carried out to investigate cytotoxic activity towards T47D, 4T1, MCF-7, HeLa, and Raji cells of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits. Zanthoxylum acanthopodium DC. METHODS: The fruit was extracted by maceration. The ethanol extract was fractionated with liquid-liquid extraction using n-hexane, chloroform at pH 3,7, and 9 to obtained alkaloid fractions. Cytotoxic activity for fraction chloroform at pH 7 and 9 was determined with MTT assay. RESULTS: The IC50 of fraction chloroform at pH 7 and 9 was (92.67 ± 1.37; 71.87 ± 1.04; 159.87 ± 0.63; 123.39 ± 0.81; and 103.09 ± 0.58 µg/mLfor pH 7) and (451.29 ± 25.48; 247.18 ± 2.82; 318.46 ± 5.40; 303.96 ± 8.75; and 181.45 ± 1.35 µg/mL for pH 9) respectively. CONCLUSION: The results reveal that alkaloid fractions at pH 7 and 9 of Zanthoxylum acanthopodium DC. Fruits have cytotoxic activity. Our further study is to isolate and assesses anticancer activity from alkaloid compounds.

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Section: Extraction and Fractionationmentioning

confidence: 99%

“…The chloroform fractions at pH 7 and 9 were carried out by thin-layer chromatography using silica gel GF 254 as stationary phase and chloroform: methanol: ammonia (18: 15: 1) as mobile phase. Dragendorff solution was used as a sprayer reagent to identify alkaloid compounds [22], [23].…”

Section: Analysis Of Alkaloids With Thin Layer Chromatographymentioning

confidence: 99%

Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits

Syari¹,

Rosidah²,

Hasibuan³

et al. 2019

Open Access Maced J Med Sci

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“…Masingmasing lapisan bawah dari pH 3, 7 dan 9 yang telah ditampung tadi di uapkan di dalam vakum rotary sampai didapat ekstrak kasar (crude isolat) dari masing-masing fraksi. Perlakuan diulangi terus-menerus sampai total ekstrak etanol yang ditimbang untuk mendapatkan ekstrak kasar (crude isolat) sebesar 50 gram [9].…”

Section: Fraksinasi Alkaloid Kasar Dari Ekstrak Etanol Kulit Batang Dan Buah Attarasaunclassified

Aktivitas Sitotoksik Fraksi Alkaloid Kulit Batang dan Buah Attarasa (Litsea cubeba Lour.) terhadap Sel Kanker Payudara T47D

Dalimunthe

Hasibuan

Silalahi

et al. 2018

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Kanker payudara adalah salah satu jenis dari kanker yang banyak menyerang wanita selain kanker mulut rahim. Tingginya angka kejadian dan resistensi agen kemoterapi menyebabkan perlu dilakukan pencarian bahan alam dengan aktivitas antikanker. Penggunaan bahan alam diharapkan dapat mengatasi resistensi dan efek samping yang terjadi ketika digunakan kemoterapi konvensional. Tujuan penelitian ini untuk mengetahui aktivitas antikanker fraksi kloroform (alkaloid) kulit batang dan buah attarasa pada sel T47D. Ekstrak etanol diekstraksi dengan cara maserasi dan difraksinasi dengan n-heksana dan kloroform pada pH 3, 7, 9. Pengujian sitotoksik secara in vitro menggunakan metode MTT yang kemudian dianalisis menggunakan SPSS 21. Hasil uji sitotoksik (IC50) yang diperoleh setelah pemberian fraksi alkaloid kulit batang dan buah attarasa pH 7 dan 9 adalah sebesar 46,60 ± 0,19; 123,01 ± 14,63 dan 35,89 ± 1,04; dan 98,31 ± 2,51 µg/mL. Fraksi alkaloid kulit batang dan buah attarasa bersifat sitotoksik terhadap sel kanker payudara T47D. Breast cancer is one type of cancer that attacks many women in addition to cervical cancer. The high incidence and resistance of chemotherapy agents causes the need to search for natural products with anticancer activities. The use of natural product is expected to overcome resistance and side effects that occur when used conventional chemotherapy. This research aimed to evaluate the anti-cancer activity of chloroform fraction (alkaloid) from cortex and Fructus of Attarasa on T47D cell. The ethanol extract was prepared by maceration method and then fractionated using n-hexane and chloroform at pH 3, 7, and 9. In vitro study of cytotoxic activity was performed using MTT method, then analyzed using SPSS 21. The IC50 values of cytotoxic activity of alkaloid fraction from cortex and Fructus of Attarasa at pH 7 dan 9 were 46.60 ± 0.19; 123.01 ± 14.63 and 35.89 ± 1.04; dan 98.31 ± 2.51 µg/mL. Alkaloid fraction from cortex skin and Fructus of Attarasa has cytotoxic activity on Breast Cancer T47D Cell

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“…Sieb et Boiss. a,b Decoction of external (pads) and leaves [19,22] Diarylheptanoid [113]; triterpenoids and flavonoid aglycones [114]; polyphenols [115] Antioxidative [115]; cytotoxic against larynx cancer cells [116] Infusion of leaves and branches [14] Several alkaloids [117][118][119] Anticholinesterase activity [120,121] 21 Poaceae Triticum aestivum L. a Decoction of shoots [20] Lignans, dietary fibers, and aleurone [122,123] Pro-apoptotic antitumor activity in colon cancer cells [122,123] 22…”

Section: Viscum Cruciatummentioning

confidence: 99%

Medicinal Plants from Jordan in the Treatment of Cancer: Traditional Uses vs.In vitroandIn VivoEvaluations – Part 1

Afifi-Yazar¹,

Kasabri²,

Abu-Dahab³

2011

Planta Med

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Bisbenzylisoquinoline alkaloids fromCocculus pendulus

Cited by 9 publications

References 16 publications

Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits

Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits

Aktivitas Sitotoksik Fraksi Alkaloid Kulit Batang dan Buah Attarasa (Litsea cubeba Lour.) terhadap Sel Kanker Payudara T47D

Medicinal Plants from Jordan in the Treatment of Cancer: Traditional Uses vs.In vitroandIn VivoEvaluations – Part 1

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