Bisdemethoxycurcumin attenuates OVA‑induced food allergy by inhibiting the MAPK and NF‑κB signaling pathways

Abstract

Bisdemethoxycurcumin (BDMC) is an important ingredient derived from turmeric in addition to curcumin. It has been reported that BDMC can be used to treat mast cell-mediated allergic diseases. In the present study, a food allergy (FA) murine model sensitized by intraperitoneal injection followed by oral challenge with ovalbumin (OVA) was established. BDMC was orally administered at 100 and 200 mg/kg for 11 days in the challenge phase to treat OVA-induced FA mice. FA symptoms such as diarrhea score, anaphylactic symptom score and rectal temperature were recorded. Intestinal tissue was also observed by hematoxylin and eosin staining. In addition, other allergic indicators were also analyzed by ELISA and western blot analysis. The present study demonstrated that BDMC could suppress the decreases in rectal temperature, diarrhea and anaphylactic symptoms in FA mice. BDMC could also ameliorate the inflammation of intestinal tissues in FA mice. BDMC not only decreased the production of OVA-specific immunoglobulin (OVA-sIg)E, IgG1, histamine, mouse mast cell protease-1, diamine oxidase, cytokines (IL-4, IL-5 and IL-13) but increased cytokines interferon-γ production. The protein expression results showed that the levels of Gata-3 were decreased but T-bet levels were increased. Furthermore, compared with the OVA group, phosphorylated (p)-p38, p-JNK, p-ERK and p-NF-κBp65 levels were decreased and p-IκBα level was increased. In conclusion, the results showed that BDMC possessed a protective effect on FA. Furthermore, BDMC was able to regulate the T-helper cells (Th)1/Th2 immune balance and inhibit the activation of MAPK and NF-κB pathways in FA mice.

Intermittent fasting protects against food allergy in a murine model via regulating gut microbiota

“…Due to the lack of IgG4 isotype ( 59 – 61 ), only total and OVA-specific IgG1 can be detected in the FA mice model. Elevated OVA-sIgG1 has been observed in FA mice model ( 54 , 62 , 63 ). We observed a similar reduction of OVA-sIgE and sIgG1 after IF when compared to a free diet.…”

The Inhibition of Mitogen-Activated Protein Kinases (MAPKs) and NF-κB Underlies the Neuroprotective Capacity of a Cinnamon/Curcumin/Turmeric Spice Blend in Aβ-Exposed THP-1 Cells

“…Our data agrees with those of Kim and collaborators [44], who showed that cinnamaldehyde produced anti-inflammatory effects in RAW 264.7 murine macrophages by inhibiting ERK, JNK and p38 MAPKs phosphorylation. Moreover, bisdemethoxycurcumin was reported to diminish the inflammatory status in a food allergy murine model sensitized after oral challenge with ovalbumin via the inhibition of MAPKs phosphorylation [45].…”

Effects of Dietary Components on Mast Cells: Possible Use as Nutraceuticals for Allergies?

“…These effects were accompanied by inhibition of the MAPK signaling pathway and the nuclear translocation of NF-κB. This mechanism may be responsible for the reduced anaphylaxis symptoms, lower diarrhea scores, and attenuated increase in rectal temperature in mice after OVA stimulation [92].…”

“…The oral administration of curcumin reduced serum histamine, PGD 2 , and LTC 4 (≈50%) levels in a mouse model of passive systematic anaphylaxis [71]. Curcumin showed a greater effect on histamine (≈70%) and OVA-specific IgE (≈80%) reduction compared to BDMC, demonstrating a less than 50% reduction in both parameters in OVA-stimulated BALB/c mice [72,92,93]. Curcumin treatment in a mouse model of allergic rhinitis reduced TNF-α cytokine levels by about 70% by suppressing the phosphorylation of the Src kinases Fyn, Lyn, and Syk [72].…”