Blockade by Polyunsaturated n-3 Fatty Acids of Endotoxin-Induced Monocytic Tissue Factor Activation Is Mediated by the Depressed Receptor Expression in THP-1 Cells

Anti-inflammatory effects of EPA and DHA are dependent upon time and dose-response elements associated with LPS stimulation in THP-1-derived macrophages

“…At the transcriptional level, these relative efficacies of EPA and DHA was also evident. Our results are supported by previous studies which have demonstrated the anti-inflammatory effects of LC n-3 PUFA on cytokine production by macrophages [33][34][35][36][37]39].…”

“…LC n-3 PUFA suppressed TNF-α expression in in vitro murine RAW 264.7 macrophages [33,34]. EPA and DHA reduced TNF-α and IL-1β in LPS-activated human monocytes and murine macrophages [35][36][37]. LC n-3 PUFA affect several components of the NF-κB transcriptional complex [34][35][36]38,39].…”

The Effects of Omega‐3 Polyunsaturated Fatty Acids on TNF‐α and IL‐10 Secretion by Murine Peritoneal Cells In Vitro

“…Other mechanisms at work have not been identified but increased activation of extracellular receptor-activated kinases 1/2 and increased binding of early growth response-1 (Egr-1) to the TNF-a promoter have been shown to contribute to the increased LPS induced TNF-a secretion by macrophages treated with ethanol [30]. Reduced activation of mitogen-activated protein kinase (P44/P42) [23], AP-1 transcription factor [3], and transcription factor nuclear factor (NF) jB [20,25] have been proposed to mediate omega-3 PUFA induced decrease in TNF-a secretion by the murine macrophage cell line, RAW 264.7 whereas reduced LPS binding, suppression of CD14 upregulation [11], reduced nuclear p65 levels, and increased IjBa expression have been proposed to mediate omega-3 PUFA induced decrease in TNF-a secretion by the human macrophage cell line, THP-1 [39].…”

The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid–bovine serum albumin complex

“…CD14 is a glycosylphosphatidylinositollinked protein and transduces its signal by associating with other partners, especially TLR4 [50,51]. It has been reported that LPS-stimulated CD14 expression and TLR-induced signaling pathways are down-regulated by x-3 fatty acids The LPS-induced pro-inflammatory response in RAW264.7 cells 157 [5,52]. In this study, the LPS-induced CD14 expression in RAW264.7 cells was only slightly attenuated by LA-BSA pre-treatment, but was increased by SA-BSA pre-treatment (Fig.…”