Blocking the large extracellular loop (LEL) domain of FcTetraspanin-3 could inhibit the infection of white spot syndrome virus (WSSV) in Chinese shrimp, Fenneropenaeus chinensis

Liu, Gui; Wang, Bing; Li, Fuhua; Sun, Yumiao; Luo, Zhan; Xiang, Jianhai

doi:10.1016/j.fsi.2012.02.022

Cited by 20 publications

(3 citation statements)

References 64 publications

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“…CD81 has also been suggested to play a role in the entry of members of the retroviridae family (human immunodeficiency virus type 1 (HIV-1) and HTLV-1) since CD81 and CD82 associate with CD4 [ 94 , 95 ]. Additionally, tetraspanins have been shown to facilitate infection of white spot syndrome virus [ 69 ], Influenza A virus [ 70 ], canine distemper virus [ 72 ], feline immunodeficiency virus [ 74 ], as well as sporozoites from Plasmodium yoelii and Plasmodium falciparum [ 61 ]. In most of these cases, tetraspanins seem to be part of a larger receptor complex assisting in the facilitation of viral entry.…”

Section: Discussionmentioning

confidence: 99%

Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus

et al. 2017

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Human cytomegalovirus (HCMV) depends on and modulates multiple host cell membrane proteins during each stage of the viral life cycle. To gain a global view of the impact of HCMV-infection on membrane proteins, we analyzed HCMV-induced changes in the abundance of membrane proteins in fibroblasts using stable isotope labeling with amino acids (SILAC), membrane fractionation and protein identification by two-dimensional liquid chromatography and tandem mass spectrometry. This systematic approach revealed that CD81, CD44, CD98, caveolin-1 and catenin delta-1 were down-regulated during infection whereas GRP-78 was up-regulated. Since CD81 downregulation was also observed during infection with UV-inactivated virus we hypothesized that this tetraspanin is part of the viral entry process. Interestingly, additional members of the tetraspanin family, CD9 and CD151, were also downregulated during HCMV-entry. Since tetraspanin-enriched microdomains (TEM) cluster host cell membrane proteins including known CMV receptors such as integrins, we studied whether TEMs are required for viral entry. When TEMs were disrupted with the cholesterol chelator methyl-β-cylcodextrin, viral entry was inhibited and this inhibition correlated with reduced surface levels of CD81, CD9 and CD151, whereas integrin levels remained unchanged. Furthermore, simultaneous siRNA-mediated knockdown of multiple tetraspanins inhibited viral entry whereas individual knockdown had little effect suggesting essential, but redundant roles for individual tetraspanins during entry. Taken together, our data suggest that TEM act as platforms for receptors utilized by HCMV for entry into cells.

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Section: Discussionmentioning

confidence: 99%

Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus

et al. 2017

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“…Compelling evidence indicates that the large extracellular loop (LEL) of mast cell-expressed TET proteins [Cluster of differentiation (CDs), such as CD9, CD63, CD81, CD82, CD151] plays a key role in the route of pathogens infection ( Pileri et al, 1998 ; Ho et al, 2006 ; Mazurov et al, 2007 ; Shanmukhappa et al, 2007 ; Singethan and Schneider-Schaulies, 2008 ). Especially, blocking the activity of large extracellular loop domain of Fenneropenaeus chinensis tetraspanin-3 by anti-LEL antibody significantly inhibit the infection of white spot syndrome virus in Chinese shrimp ( Gui et al, 2012 ). In animals, the EC2 domain of TETs may be relevant for the protein complex formation with other proteins ( Hemler, 2001 , 2003 ; Stipp et al, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Tetraspanins Facilitate Virus Infection via Membrane-Recognition GCCK/RP Motif and Cysteine Residues

Zhu

Sun

2022

Front. Plant Sci.

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Tetraspanins (TETs) function as key molecular scaffolds for surface signal recognition and transduction via the assembly of tetraspanin-enriched microdomains. TETs’ function in mammalian has been intensively investigated for the organization of multimolecular membrane complexes, regulation of cell migration and cellular adhesion, whereas plant TET studies lag far behind. Animal and plant TETs share similar topologies, despite the hallmark of “CCG” in the large extracellular loop of animal TETs, plant TETs contain a plant specific GCCK/RP motif and more conserved cysteine residues. Here, we showed that the GCCK/RP motif is responsible for TET protein association with the plasma membrane. Moreover, the conserved cysteine residues located within or neighboring the GCCK/RP motif are both crucial for TET anchoring to membrane. During virus infection, the intact TET3 protein enhanced but GCCK/RP motif or cysteine residues-deficient TET3 variants abolished the cell-to-cell movement capability of virus. This study provides cellular evidence that the GCCK/RP motif and the conserved cysteine residues are the primary determinants for the distribution and function of TET proteins in Arabidopsis.

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“…Silencing of caspase‐3 and Toll gene in L. vannamei activated an antiviral response against WSSV (Rijiravanich et al ; Lebreuche et al ). Silencing of tetraspanin‐3 in Fenneropenaeus chinensis significantly reduced mortality in shrimp challenged with WSSV (Gui et al ). In addition, Rab7 gene silencing has shown an impact against the replication of WSSV in P. monodon juveniles (Ongvarrasopone et al ).…”

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confidence: 99%

Long‐lasting Effect Against White Spot Syndrome Virus in Shrimp Broodstock, Litopenaeus vannamei, by LvRab7 Silencing

Álvarez-Ruíz

Luna‐González

Escamilla-Montes

et al. 2015

J World Aquaculture Soc

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The white spot syndrome virus (WSSV) remains the most devastating viral pathogen of shrimp culture worldwide. Gene silencing by RNA interference (RNAi) using double stranded RNA (dsRNA) has been considered a powerful tool for conferring protection against WSSV when viral genes are silenced, as documented in several shrimp species. However, this effect is not long lasting. Our results provide the first evidence that long-term silencing of the LvRab7 endogen produced antiviral effect against WSSV, which endured at least 21 d after dsRNA treatment (dat). Until now, the most efficient way to implement RNAi with dsRNA into the shrimp is by injection. Consequently, its application to broodstock in hatcheries is possible, minimizing the risk of vertical transmission of the virus. We show that the expression of Rab7 in hemocytes is lowest at 2 dat and finally recovers to basal status. In contrast, in gills and pleopods, gene expression silencing continued for at least 21 d. We challenged Litopenaeus vannamei broodstock with WSSV at 7, 14, or 21 dat reaching mortality rates of 0, 40, and 27%, respectively. In conclusion, the LvRab7 gene silencing is progressive and effective against WSSV. However, further studies are necessary to elucidate the functions of Rab7 in shrimp cells before applying this methodology at a commercial level.

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Blocking the large extracellular loop (LEL) domain of FcTetraspanin-3 could inhibit the infection of white spot syndrome virus (WSSV) in Chinese shrimp, Fenneropenaeus chinensis

Cited by 20 publications

References 64 publications

Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus

Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus

Arabidopsis Tetraspanins Facilitate Virus Infection via Membrane-Recognition GCCK/RP Motif and Cysteine Residues

Long‐lasting Effect Against White Spot Syndrome Virus in Shrimp Broodstock, Litopenaeus vannamei, by LvRab7 Silencing

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