Blood and Tissue Enzymatic Activities of GDH and LDH, Index of Glutathione, and Oxidative Stress among Breast Cancer Patients Attending Referral Hospitals of Addis Ababa, Ethiopia: Hospital‐Based Comparative Cross‐Sectional Study

Mehdi, Mohammed; Menon, M.K.; Seyoum, Nebiyou; Bekele, Mahteme; Tigeneh, Wondimagegn; Seifu, Daniel

doi:10.1155/2018/6039453

Cited by 25 publications

(17 citation statements)

References 40 publications

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“…Further significant increases in these levels occur after radiotherapy. These results are consistent with that of Mehdi et al who reported that the level of TAC of breast cancer patients wassignificantly lowerthan that of the healthy control group, while concentration of TOS among breast cancer patients were significantly higher than among the control group (Mehdi et al, 2018).…”

Section: Discussionsupporting

confidence: 93%

Oxidative Stress Parameters in Patients with Breast Cancer before and after Radiotherapy

Youssef

Salem

2019

Egypt. J. Rad.Sci.

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R EACTIVE oxygen species (ROS) are generated in response to endogenous or exogenous stimuli. Antioxidants defense system resists for balancing ROS-mediated injury; if oxidation exceeds the defense mechanisms, oxidative stress is generated. Oxidative stress may be involved in the development of breast cancer. Moreover, radiationtherapy (RT), used for the treatment of breast cancer, works by the production of reactive oxygen species at the site of radiation which leads to local oxidative stress. Studies which detect one or few oxidant and antioxidant markers failed to detect the overall oxidant/antioxidant status of the subjects. The authors aim at studying the impact of radiotherapy on the total oxidant status (TOS), total antioxidant status (TAS) with calculation of oxidative stress index (OSI), and measure the lipid peroxidation (MDA) in breast cancer patients. TAS, TOS, MDA and OSI in healthy and breast cancer groups are measured. In breast cancer group, all parameters were measuredbefore and after radiation therapy. In the breast cancer group, TOS, OSI and MDA levels have increased significantly (P<0.001) and the TAS level has decreased (P<0.001) in the breast cancer patients after radiotherapy than before radiotherapy. Breast cancer group TAS after RT reaches about forth its level measured inthe control group. Radiotherapy in breast cancer patients depletes the total antioxidants (TAS), increases total oxidative status (TOS), lipid peroxidation (MDA) and OSI. Breast cancer and its treatment modalities display the patients in a state of severe oxidative stress which requires the supplementation of antioxidants.

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Section: Discussionsupporting

confidence: 93%

Oxidative Stress Parameters in Patients with Breast Cancer before and after Radiotherapy

Youssef

Salem

2019

Egypt. J. Rad.Sci.

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“…Others have found similar results when evaluating antioxidant status in the peripheral blood of patients with gastric cancer [ 34 – 36 ]. Although some studies have demonstrated good correlation of increased oxidative stress in the serum and cancerous tissue samples [ 37 ], others have shown poor correlation [ 38 ]. Therefore, abnormal serum oxidative status in our study may not reflect oxidative stress status in the gastric tissue.…”

Section: Discussionmentioning

confidence: 99%

Increased Oxidative Stress in Gastric Cancer Patients and Their First‐Degree Relatives: A Prospective Study from Northeastern Brazil

Neto

Costa

Queiroz

et al. 2021

Oxidative Medicine and Cellular Longevity

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Background and Aims. First-degree relatives of gastric cancer patients are at increased risk of developing gastric cancer. Increased oxidative stress, including lipid peroxidation, has been associated with gastric carcinogenesis. Whether first-degree relatives of gastric cancer patients have increased oxidative stress remains unknown. We aimed to compare oxidative stress in patients with gastric cancer, their first-degree relatives, and dyspeptic controls. Methods. A total of 155 patients undergoing upper endoscopy were prospectively enrolled, including 50 with gastric cancer, 49 first-degree relatives of gastric cancer patients, and 56 controls. Serum concentrations of malondialdehyde (MDA) and glutathione) and activities of superoxide dismutase (SOD) and catalase were measured. Multivariate analysis adjusting for sex, age, smoking status, and alcohol consumption was performed. Results. Lipid peroxidation, as measured by concentration of MDA (nmol/mL), was higher ( p = 0.04 ), and glutathione levels were lower ( p < 0.001 ) in the gastric cancer group compared to controls. There was no difference in the catalase activity among the groups. There was no difference in glutathione and MDA concentration or catalase activity between the different stages of gastric cancer based on the TNM classification. Relatives of gastric cancer patients had higher glutathione concentration (μmol/mL) compared to gastric cancer patients (262.5 vs. 144.6; p = 0.018 ), while there was no difference in MDA concentration. Catalase and superoxide dismutase activity were lower in the gastric cancer group (3.82 vs. 0.91; p < 0.001 and 1.04 vs. 0.6; p < 0.001 ) compared to their first-degree relatives. Interestingly, MDA concentration in the first-degree relative group was higher than in the control group (7.9 vs. 5.1; p = 0.03 ). Conclusions. In this study, similarly to gastric cancer patients, their first-degree relatives were found to have increased oxidative stress compared to controls. Further studies are warranted to validate this observation and to better understand the role of oxidative stress as a possible biomarker in this population.

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“…MDA is a metabolic product of lipid peroxidation, and the content of it can indicate the degree of lipid peroxidation in cells (J. Xu et al, 2018). The release of LDH is seen as a mark of damaged cells, and any kind of cell injury can cause a leakage (Mehdi et al, 2018). The association between LDH and increased oxidative stress has been revealed in much pathology (Amantea et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Neobavaisoflavone protects osteoblasts from dexamethasone‐induced oxidative stress by upregulating the CRNDE‐mediated Nrf2/HO‐1 signaling pathway

Zhu

Wang

et al. 2021

Drug Development Research

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Neobavaisoflavone (NBIF) is a flavonoid, which has a variety of pharmacological activities. However, the mechanism of NBIF in the treatment of osteoporosis still needs further exploration. The differentiation of osteoblast MC‐3T3‐E1 cells after treatment was observed by Alizarin red staining. Cell counting kit‐8 and flow cytometry were used to detect viability, apoptosis, and reactive oxygen species (ROS) levels of treated MC‐3T3‐E1 cells, respectively. Malondialdehyde (MDA), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) were tested by ELISA kits. The expressions of lncRNA MALAT1, MEG3, CRNDE, Runx2, osteocalcin (OCN), osteopontin (OPN), collagen I (col‐I), nuclear Nrf2, cytoplasm Nrf2, heme oxygenase‐1 (HO‐1) and quinone oxidoreductase 1 (NQO1) in treated MC‐3T3‐E1 cells were examined by Quantitative real‐time PCR or Western blot. Dexamethasone (Dex) inhibited the viability of MC‐3T3‐E1 cells, while the appropriate amount of NBIF had no significantly effect on cell viability. Dex downregulated CRNDE expression, whereas NBIF upregulated CRNDE. Overexpressed CRNDE and NBIF reversed the inhibitory effects of Dex on cell viability, differentiation and levels of SOD, GSH‐Px, Runx2, OCN, OPN, col‐I, nuclear Nrf2, HO‐1 and NQO1 while reversing the promoting effect of Dex on apoptosis and the levels of ROS, MDA, LDH and cytoplasm Nrf2 in MC‐3T3‐E1 cells, respectively, but shCRNDE further reversed the effects of NBIF in MC‐3T3‐E1 cells. NBIF protected osteoblasts from Dex‐induced oxidative stress by upregulating the CRNDE‐mediated Nrf2/HO‐1 signaling pathway.

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Blood and Tissue Enzymatic Activities of GDH and LDH, Index of Glutathione, and Oxidative Stress among Breast Cancer Patients Attending Referral Hospitals of Addis Ababa, Ethiopia: Hospital‐Based Comparative Cross‐Sectional Study

Cited by 25 publications

References 40 publications

Oxidative Stress Parameters in Patients with Breast Cancer before and after Radiotherapy

Oxidative Stress Parameters in Patients with Breast Cancer before and after Radiotherapy

Increased Oxidative Stress in Gastric Cancer Patients and Their First‐Degree Relatives: A Prospective Study from Northeastern Brazil

Neobavaisoflavone protects osteoblasts from dexamethasone‐induced oxidative stress by upregulating the CRNDE‐mediated Nrf2/HO‐1 signaling pathway

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