Blood Group Antigens on Human Spermatozoa

Edwards, R. G.; Ferguson, Lucy; Coombs, R.R.A.

doi:10.1530/jrf.0.0070153

Cited by 82 publications

(38 citation statements)

References 16 publications

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“…In agreement with Edwards et al (1964), Boettcher (1965 reported, using the inhibition technique, that spermatozoa absorb blood group antigens from seminal plasma, i.e., spermatozoa from 0 non-secretor incubated in seminal plasma from A secretor absorb anti-A serum and anti-H agglutinins. In the experiment of Boettcher (1965), spermatozoa incubated in seminal plasma were washed in saline only three times.…”

Section: Methodssupporting

confidence: 88%

“…The cause of low rate may be attributed to the preparation procedure, since methanol fixation is considered to remove components of the outer membranes of spermatozoa (Hjort and Hansen 1977) and multiple washings of spermatozoa are recognized to remove some membrane fragments from spermatozoa. Edwards et al (1964), using the mixed agglutination technique, failed to detect A and B antigens on spermatozoa from non-secretor, though they detected them on spermatozoa from secretors. This seems to indicate only that the group specific antigens from seminal plasma were demonstrated.…”

Section: Methodsmentioning

confidence: 97%

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ABO blood group antigens of human spermatozoa.

Takeda

Hiraiwa

1985

Tohoku J. Exp. Med.

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ABO blood group antigens on the membrane of human spermatozoa were investigated with the peroxidase-labelled antibody test. Spermatozoa from 0 secretor were incubated with saliva or seminal plasma of A and B secretors, but the specific peroxidase staining was negative. This result indicates that blood group antigens on human spermatozoa originate from spermatozoon itself, but not from seminal plasma. ABO blood group antigens ; human spermatozoa ; peroxidase-labelled antibody test Yamakami (1926) reported that spermatozoa have a specific inhibiting action on the human isoagglutinin, and in the same year Landsteiner and Levine (1926) demonstrated the presence of A and B blood group antigens on human spermatozoa using the inhibition technique. Thereafter, several investigators obtained similar results with various methods. Levine and Celano (1961), using the absorption-elution method, re-confirmed these results with spermatozoa from both secretor and non-secretor in an attempt to detect D (Rho) antigen in sperm cells. Shahani and Southam (1962), using the fluorescent antisera, supported the presence of blood group antigens on human spermatozoa. Recently, Inoue and Okada (1982, 1983) demonstrated ABO blood group antigens on the membrane of human spermatozoa using both enzyme and ferritin-labelled antibody techniques. However, there still remains a question whether ABO blood group antigens on the membrane of spermatozoa originate from seminal plasma or not. The present study deals with the origin of ABO antigens on human spermatozoa. MATERIALS and METHODSPreparation of samples. Semen samples were obtained from 13 donors of known blood groups (A secretor 7, B secretor 3, B non-secretor 1, AB secretor 1, and 0 secretor 1), and kept frozen at -40°C until use. One ml of the semen was washed twelve times in 10 ml of 0.01 M phosphate buffered saline (PBS), adjusted to pH 7.2, by centrifugation at 3,000 rpm for 10 min and the deposit was used as washed spermatozoa. The supernatant of twelfth washing was confirmed not to include water-soluble antigens, using either hemagglutination inhibition test or enzyme-labelled antibody test, the latter being performed by the method

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Section: Methodssupporting

confidence: 88%

Section: Methodsmentioning

confidence: 97%

ABO blood group antigens of human spermatozoa.

Takeda

Hiraiwa

1985

Tohoku J. Exp. Med.

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“…Although some workers have reported finding the D antigen on spermatozoa (Gullbring, 1957; see also Levine & Celano, 1961;Quinlivan & Masouredis, 1962), Lawler & Shatwell (1962) have suggested that the D antigen is unlikely to be found anywhere but on the human erythrocyte. Popivanov & Vulchanov (1968) agreed with the sug¬ gestion of Edwards et al (1964) …”

Section: Introductionmentioning

confidence: 59%

“…In the mixed agglutination which they observed using human spermatozoa, O erythrocytes and anti-human O erythrocyte antiserum, Edwards et al (1964) found that the distribution of the antigen (s) involved in the agglutination was uniform over the head and tail of the sperm cells. Although Edwards et al (1964) (Weil, 1967). Alternatively, the antigens may:…”

Section: Bleeding Of Animalsmentioning

confidence: 98%

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Examination of the Semen and Erythrocytes of Seven Species for Immunological Cross-Reaction

Baldo¹,

Boettcher²

1971

Reproduction

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Cell surface changes in the proteins of rabbit spermatozoa during epididymal passage

et al. 1979

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Differences in the exposure of spermatozoa surface components during epididymal passage have been examined using lactoperoxidase‐catalyzed 125I‐iodination or labeling with 125I‐diazodiiodosulfanilic acid. Labeled surface proteins obtained from caput and cauda epididymides were solubilized in detergent, separated by sodium dodecylsulfate polyacrylamide slab gel electrophoresis, and identified by radiography. Densitometer scans of autoradiograms revealed increased amounts or exposures of surface proteins of ∼35,000, ∼39,000, ∼50,000, and ∼78,000 molecular weight on the cauda epididymal spermatozoa.

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Blood Group Antigens on Human Spermatozoa

Cited by 82 publications

References 16 publications

ABO blood group antigens of human spermatozoa.

ABO blood group antigens of human spermatozoa.

Examination of the Semen and Erythrocytes of Seven Species for Immunological Cross-Reaction

Cell surface changes in the proteins of rabbit spermatozoa during epididymal passage

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