Blood levels of nitric oxide and DNA breaks assayed in whole blood and isolated peripheral blood mononucleated cells in patients with multiple sclerosis

Borisovs, Vitālijs; Ļeonova, Elīna; Баумане, Л.; Kalnina, Jolanta; Mjagkova, Natalja; Sjakste, Nikolajs

doi:10.1016/j.mrgentox.2018.11.008

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…Nitrites and nitrates in plasma and serum were measured using the Sievers NOA-280i chemiluminescence detector, and MDA levels in plasma and serum were assessed via TBARS assay. Detailed methods and results for these assays can be found in previous studies [11], [12].…”

Section: Biochemical Markersmentioning

confidence: 99%

“…A cohort study was conducted, comprising 96 patients who underwent SNP genotyping to investigate the role of genetic markers in multiple sclerosis (MS). Of these patients, 26 were previously included in a biochemical analysis study [11], [12]. The study group consisted of 78.1% females and 21.9% males, re ecting the well-known female predisposition to MS [13].…”

Section: Patientsmentioning

confidence: 99%

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Investigating the Correlation between Genetic and Biochemical Markers in Multiple Sclerosis

Borisovs

Trapiņa

Paramonova

et al. 2023

Preprint

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Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system with a complex etiology involving genetic and environmental factors. Genome-wide association studies have identified several genetic variants associated with MS susceptibility, including the XRCC1 gene involved in DNA base excision repair. Environmental factors such as exposure to nitric oxide have also been linked to MS development and progression. This study aims to investigate the potential association between these factors, including patient demographic descriptors and biochemical markers, to better understand the mechanisms underlying MS. This scientific article discusses the results of statistical analysis of biomarkers in relation to multiple sclerosis (MS). Our findings reveal significant associations between various factors, such as levels of nitric oxide (NO) and serum nitrite levels, NO and plasma malondialdehyde (MDA) levels, nitrite and nitrate levels in serum, and nitrite and MDA levels in plasma. It also suggests a potential association between XRCC1 rs25487 T > C locus deviation from Hardy-Weinberg equilibrium and MS in the population of Latvia. Furthermore, XRCC1 rs25487 T > C is found to be statistically significantly associated with DNA damage in peripheral blood mononuclear cells (PBMNC) and potentially associated with levels of NO. Additionally, PARP1 rs1136410 A > G is linked to plasma levels of MS patients. Finally, the study finds that XRCC1 rs25487 T > C is associated with both plasma nitrite levels and serum malondialdehyde (MDA) levels of MS patients, with the rare allele T specifically correlated with the latter.

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Section: Biochemical Markersmentioning

confidence: 99%

Section: Patientsmentioning

confidence: 99%

Investigating the Correlation between Genetic and Biochemical Markers in Multiple Sclerosis

Borisovs

Trapiņa

Paramonova

et al. 2023

Preprint

Self Cite

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“…Each of them was analysed in duplicates -two samples per slide. The general procedure for alkaline comet assay was previously described (Borisovs et al, 2019) and performed with a few modi cations. 50 µL of isolated PBMNC were mixed with 100 µL of 1% low-melting agarose preheated to 37 o C (Sigma-Aldrich, Germany).…”

Section: Collection and Processing Of Blood Samplesmentioning

confidence: 99%

“…Taking into account the total number of the registered MS patients in Latvia ( 2035), 1.2% of all patients were enrolled in the study. (Borisovs et al, 2019). To evaluate the number of oxidized bases, DNA damage data was subtracted from the enzyme induced damage data.…”

Section: Demographic Datamentioning

confidence: 99%

Nitrosative stress parameters and the level of oxidized DNA bases in patients with multiple sclerosis

et al. 2021

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Multiple sclerosis (MS) is a neurodegenerative disease with various factors affecting its etiology.Overproduction of nitric oxide and subsequent lesions of biopolymers are some of the possible causes of the disease. This study aimed to measure the most relevant nitrosative and oxidative stress biomarkers and the level of modi ed DNA bases in patients with MS. Each parameter was assayed in 25 patients with MS and 25 healthy controls. This study involved detecting blood plasma and serum nitric oxide metabolites by chemiluminescence detector Sievers NOA-280i, malondialdehyde (MDA) measurements with thiobarbituric acid reactive substance (TBARS) assay, detection of oxidized purines and pyrimidines with the enzyme-modi ed comet assay. Statistical analysis of the results was performed by one-way analysis of variance (ANOVA) and unpaired t test for the comparison of less than three data sets. DNA single-strand breaks, levels of modi ed purines and pyrimidines, as well as nitrite and nitrate levels in plasma and serum samples, were signi cantly higher in patients with MS than in healthy controls. On the contrary, MDA levels appeared to be lower in patients with MS.

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“…Measurement of NO in whole blood. NO production was measured essentially as described (24). A total of 20 mg diethylthiocarbamate (Sigma-Aldrich; Merck KGaA) was added to 1 ml fresh blood, stirred and incubated for 30 min at room temperature.…”

Section: Patients and Ethicsmentioning

confidence: 99%

Nitric oxide metabolism is impaired by type 1 diabetes and diabetic nephropathy

et al. 2020

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Blood levels of nitric oxide and DNA breaks assayed in whole blood and isolated peripheral blood mononucleated cells in patients with multiple sclerosis

Cited by 12 publications

References 27 publications

Investigating the Correlation between Genetic and Biochemical Markers in Multiple Sclerosis

Investigating the Correlation between Genetic and Biochemical Markers in Multiple Sclerosis

Nitrosative stress parameters and the level of oxidized DNA bases in patients with multiple sclerosis

Nitric oxide metabolism is impaired by type 1 diabetes and diabetic nephropathy

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