Blood Monocyte Subpopulations During Uncomplicated Coronary Artery Bypass Surgery

Головкин, Сергей Иванович; Матвеева, Г.; Кудрявцев, В. А.; Григорьев, В.; Великанова, А.; Байракова, В.

doi:10.15789/1563-0625-2012-4-5-305-312

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Субпопуляция классических моноцитов определяется как крупные клетки с высоким уровнем фагоцитарной активности. Для них характерен повышенный уровень экспрессии таких рецепторов, как CCR2, CD36, CD64, CD62L и низкий уровень синтеза TNFα и IL-1 [1,16]. Неклассические моноциты представлены некрупными клетками с низкой фагоцитарной и оксидазной активностью.…”

Section: Discussionunclassified

“…Однако необходимо отметить, что и сами моноциты, также как и их потомки -макрофаги и дендритные (стимулирующие развитие адаптивного иммунного ответа в качестве антигенпрезентирующих клеток), являются регуляторными клетками, в том числе, и для клеток врожденного иммунитета [2,8,24]. К тому же моноциты, долгое время рассматривающиеся в качестве моноклеточной популяции, на сегодняшний день, в зависимости от экспрессии CD16рецептора, делятся на две основные субпопуляции: классические моноциты (CD14 + CD16 -) и неклас-сические (CD14 + CD16 + ) [1,6,16]. При этом регуляторная роль субпопуляций моноцитов на гранулоцитарные клетки крови при РГП до сих пор не изучена.…”

Section: Introductionunclassified

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Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Савченко¹,

Борисов²,

Черданцев³

et al. 2018

Russian Journal of Infection and Immunity

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The aim of the study was to investigate the regulatory effect of monocytes and their subpopulations on the population composition of granulocyte leukocytes and the state of their respiratory burst in widespread purulent peritonitis (WPP). The study involved 24 patients aged 30-65 with acute surgical diseases and injuries of abdominal organs complicated by WPP. As a control 25 relatively healthy people of the same age range were examined. A study of the population composition of monocytes and granulocyte leukocytes in blood was performed using a two-platform technology on the hematological analyzer Sysmex XE-5000 (Sysmex Inc., USA) and FC-500 flow cytometer (Beckman Coulter, USA) using the Cytodiff antibody kit (Beckman Coulter, USA). A study of the monocytes number expressing HLA-DR- and CD64-receptor was performed by flow cytometry using direct immunofluorescence of whole peripheral blood. The respiratory burst state of neutrophilic granulocytes was studied by chemiluminescence analysis on a 36-channel chemiluminescence analyzer BLM-3607 (MedBioTech, Russia). As indicators of chemiluminescence were used luminol and lucigenin. The enhancement of chemiluminescence induced by zymosan was evaluated by the ratio of the area of the induced chemiluminescence to the spontaneous area and was defined as the activation index. It has been established that the immune-inflammatory process in WPP is characterized by a decrease in the number of classical monocytes in the peripheral blood and an increase in the content of non-classical monocytes. In WPP in peripheral blood the level of monocytes expressing HLA-DR receptors decreases. The change in the ratio of monocytes subpopulations characterizes the increase in the role of the proinflammatory fraction in the WPP pathogenesis. Changes in the population composition of granulocytes in the blood in patients with WPP also characterize the development of an acute inflammatory process. In this case, there is a decrease in the number of basophils in the peripheral blood, which, apparently, is determined by the presence of an allergic component in WPP and, accordingly, their migration to the inflammation area. In patients with WPP activation of a respiratory burst of granulocytes of blood was detected, the intensity of which is determined by the synthesis of primary and secondary active oxygen species. The results of the correlation analysis made it possible to establish that in WPP the regulatory role of non-classical monocytes increases aimed at stimulating the inflammatory processes (an increase in the number of mature and immature forms of neutrophils and stimulation of the activity of a respiratory explosion of granulocytes). The revealed features of the regulatory effect of monocytes on the population composition and the intensity of the respiratory burst of granulocytes can be used in the development of immunotherapeutic methods aimed at reducing the activity of the inflammatory process in WPP.

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Section: Discussionunclassified

Section: Introductionunclassified

Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Савченко¹,

Борисов²,

Черданцев³

et al. 2018

Russian Journal of Infection and Immunity

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“…CD16-антиген является низкоаффинным Fcрецептором для IgG [9,14,19]. Моноциты, не экспрессирующие CD16-антиген, определяются как «классические» [5,8,18]. Данная фракция представлена крупными клетками с высоким уровнем фагоцитарной активности.…”

Section: Discussionunclassified

Blood Leukocytes Phenotyping by Hematoflow Method in Patients With Onychomycosis

Савченко

Борисов

Анисимова

et al. 2016

Russian Journal of Infection and Immunity

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Инфекция и иммунитет А.А. Савченко и др. врожденного и адаптивного иммунитета. Он позволяет оценивать степень тяжести иммунопатологического процесса, механизм и уровень повреждения иммунной системы, можно рекомендовать его применение для персонифицированного подхода к назначению иммунотропного лечения.

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Immunity and Its Effect on the Incidence of Multiple Organ Failure in Patients after the Heart Surgery

Партылова¹,

Петрищев²,

Кудрявцев³

et al. 2019

Obŝaâ reanimatologiâ

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The purpose of the study is to identify the components of immunity, which might predict the development of multiple organ failure in patients after heart surgery.Material and methods. The study included 40 patients who were operated in cardiac surgery Department. The inclusion criteria were the presence of indications for cardiac surgery. The exclusion criteria were the presence of infective endocarditis in patients. Before the operation and after 24 hours we studied the blood level of leukocytes and lymphocytes. We analyzed the phenotype of immune cells using monoclonal antibodies, serum levels of procalcitonin and C3 and C4 complement components. All patients were evaluated for multiple organ disfunction (MOD) using the SOFA scale.The results showed that cardiac surgery leads to the development of MOD, statistically significant multidirectional changes in both quantitative and qualitative composition of all cells of the immune system, significant changes in the level of C3 and C4 components of the cascade of complement and plasma level of pro-calcitonin. ROC analysis was revealed that the relative content of monocytes is less than 7.1% of the number of leukocytes as well as the absolute content of monocytes with the CD14 + HLA-DR + phenotype less than 0.32_109/l in the preoperative period, and the C3 level of the complement component less than 0.52 g/l, as well as the maximum SOFA score in the postoperative period, were the best predictors of MOD after the procedures.Conclusion. The components of innate immunity make it possible to predict the complication of the cardiac surgery, earlier than the SOFA scale.

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Blood Monocyte Subpopulations During Uncomplicated Coronary Artery Bypass Surgery

Cited by 4 publications

References 6 publications

Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Regulatory Influence of Blood Monocytes on the Population Composition of Granulocytes and the State of Their Respiratory Burst in the Widespread Purulent Peritonitis

Blood Leukocytes Phenotyping by Hematoflow Method in Patients With Onychomycosis

Immunity and Its Effect on the Incidence of Multiple Organ Failure in Patients after the Heart Surgery

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