Blood platelet and monocyte activations and relation to stages of liver cirrhosis

Panasiuk, Anatol; Zak, Janusz; Kasprzycka, Edwina; Janicka, K; Prokopowicz, D

doi:10.3748/wjg.v11.i18.2754

Cited by 36 publications

(30 citation statements)

References 30 publications

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“…Similar results were reported by Camps et al [26] . However, Panasiuk et al [27] reported a de- Our results also revealed a significant negative correlation between vitamin D and MCP-1. This supports the role of decreased vitamin D in inflammation and fibrosis.…”

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confidence: 60%

Relationship between vitamin D and IL-23, IL-17 and macrophage chemoattractant protein-1 as markers of fibrosis in hepatitis C virus Egyptians

Husseiny

Fahmy²,

Mohamed³

et al. 2012

WJH

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AIM:To assess vitamin D in hepatitis C patients and its relationship to interleukin (IL)-23, IL-17, and macrophage chemoattractant protein-1 (MCP-1). METHODS:The study was conducted on 50 Egyptian hepatitis C virus (HCV) genotype number IV-infected patients and 25 age-and gender-matched healthy subjects. Venous blood samples were obtained. Samples were allowed to clot and sera were separated by centrifugation and stored at -20 ℃. A 25 hydroxy vitamin D assay was carried out using solid phase RIA. A 1,25 dihydroxy vitamin D assay was carried out using a commercial kit purchased from Incstar Corporation. IL-17 and -23 and MCP-1 were assayed by an enzyme immunoassay. Quantitative and qualitative polymerase chain reaction for HCV virus were done by TaqMan technology. Only HCV genotype IV-infected subjects were included in the study. The mean ± SD were determined, a t -test for comparison of means of different parameters was used. Correlation analysis was done using Pearson's correlation. Differences among different groups were determined using the Kruskal-Wallis test. RESULTS:The mean vitamin D level in HCV patients (group Ⅰ) was 15 ± 5.2 ng/mL while in control (group Ⅱ) was 39.7 ± 10.8. For active vitamin D in group Ⅰ as 16.6 ± 4.8 ng/mL while in group Ⅱ was 41.9 ± 7.9. IL-23 was 154 ± 97.8 in group Ⅰ and 6.7 ± 2.17 in group Ⅱ. IL-17 was 70.7 ± 72.5 in cases and 1.2 ± 0.4 in control. MCP-1 was 1582 ± 794.4 in group Ⅰ and 216.1 ± 5.38 in group Ⅱ. Vitamin D deficiency affected 72% of HCV-infected patients and 0% of the control group. Vitamin D insufficiency existed in 28% of HCV-infected patients and 12% of the control group. One hundred percent of the cirrhotic patients and 40% of non cirrhotic HCV-infected patients had vitamin D deficiency. IL-23, IL-17, and MCP-1 were markedly increased in HCV-infected patients in comparison to controls.A significant negative correlation between vitamin D and IL-17 and -23 and MCP-1 was detected. HCV-infected males and females showed no differences with respect to viral load, vitamin D levels, IL-17, IL-23 and MCP-1. The viral load was negatively correlated with vitamin D and active vitamin D (P = 0.0001 and P = 0.001, respectively), while positively correlated with IL-23, IL-17, and MCP-1. We classified the patients according to sonar findings into four groups. Group Ⅰa with bright hepatomegaly and included 14 patients. Group Ⅰb with perihepatic fibrosis and included 11 patients. Group Ⅰc with liver cirrhosis and included 11 patients. Group Ⅰd with hepatocellular carcinoma (HCC) and included 14 patients. Vitamin D and active vitamin D were shown to be lower in cirrhotic patients and much lower in patients with HCC, and this difference was highly significant (P = 0.0001). IL-17 and -23 and MCP-1 were higher in advanced liver disease) and the differences were highly significant (P = 0.0001). ORIGINAL ARTICLE

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confidence: 60%

Relationship between vitamin D and IL-23, IL-17 and macrophage chemoattractant protein-1 as markers of fibrosis in hepatitis C virus Egyptians

Husseiny

Fahmy²,

Mohamed³

et al. 2012

WJH

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“…All of the seven patients with liver disease suffered from invasive aspergillosis, which reflected their immunocompromised status and the presence of additional risk factors for S. maltophilia colonization, such as antibiotic therapy. As such, those seven patients are at risk of infections precipitated by the significant impairment of the neutrophil defence mechanism, frequent use of corticosteroids and invasive procedures, and malnutrition (Panasiuk et al, 2005;Cheruvattath & Balan, 2007;Falcone et al, 2011;Fishman, 2011;Barchiesi et al, 2015). However, to the best of our knowledge, our study is the first description of an association between co-infection by A. fumigatus and S. maltophilia and patients with liver disease.…”

Section: Discussionmentioning

confidence: 71%

Concomitant presence of Aspergillus fumigatus and Stenotrophomonas maltophilia in the respiratory tract: a new risk for patients with liver disease?

Cabaret

Bonnal

Canouï‐Poitrine

et al. 2016

Journal of Medical Microbiology

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Concomitant lung colonization by Aspergillus fumigatus and Stenotrophomonas maltophilia was reported mainly in patients with cystic fibrosis (CF) and immunocompromised patients. The aim of the study was to assess the frequency of co-culture of A. fumigatus and S. maltophilia in respiratory samples of hospitalized patients, and to determine its associated factors. Between 2007 and 2011, all patients who had A. fumigatus in their respiratory samples were retrospectively enrolled in the study. Their clinical and laboratory data, including the presence of S. maltophilia in a respiratory sample, were collected within the same month. Of the 257 enrolled patients (372 respiratory samples), 71 % were immunocompromised and 32 % had chronic respiratory disease. S. maltophilia was isolated within the same month in 20 patients (7.8 %). In the univariate analysis, factors associated with concomitant culture of A. fumigatus and S. maltophilia were liver disease (P50.009), orotracheal intubation (P50.001), ventilator-associated pneumonia (P50.006), central venous catheter (P50.003), parenteral nutrition (P50.008) and culture of Pseudomonas aeruginosa in respiratory samples (P50.002). In the multivariate analysis, the simultaneous presence of P. aeruginosa in the respiratory tract (odds ratio (OR)53.19, 95 % confidence interval (CI) 1.11-9.14, P50.031), liver disease (OR53.92, 95 % CI 1.32-11.62, P50.014) and orotracheal intubation (OR53.42, 95 % CI 1.17-9.96, P50.024) were independently associated with the co-culture of S. maltophilia and A. fumigatus. Factors independently associated with the concomitant culture of A. fumigatus and S. maltophilia were identified. These results support a future prospective study focusing on liver disease and its complications.

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“…Blood platelets, besides hemostatic properties, have the features of inflammatory cells [15,16]. Activated platelets present on their surfaces molecules CD62P and CD63 that transmit stimulation signals when in contact with other cells (platelets, monocytes, and lymphocytes) [17].…”

Section: Discussionmentioning

confidence: 99%

Increase in expression of monocytic tissue factor (CD142) with monocytes and blood platelet activation in liver cirrhosis

Panasiuk

Zak

Panasiuk

et al. 2007

Blood Coagulation &Amp; Fibrinolysis

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Tissue factor (TF) is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of TF, and while accumulating in the organ they can intensify inflammation. The aim of the present study was to evaluate the expression of TF on monocytes in advanced liver cirrhosis with regard to other activation markers. The flow cytometric analysis of TF (CD142), CD14, adhesive molecules CD11b and CD11c, costimulatory molecules CD40, CD80 and CD86, and HLA-DR on monocytes was carried out in 45 patients with postalcoholic liver cirrhosis (Child Pugh B, 20 patients; Child Pugh C, 25 patients) and in 25 healthy persons. The positive correlation between monocytic TF expression and monocyte [soluble CD14 (sCD14), CD11b, monocyte aggregates] activation, the expression of costimulatory molecules on monocytes (CD40, CD80), blood platelet (soluble P-selectin, microplatelets) activation, the level of tumor necrosis factor-alpha, biochemical parameters of liver damage (alanine aminotransferase, aspartate aminotransferase, alkaline phosphate, gamma-glutamyltransferase, and bilirubin) as well as coagulation disorders were observed in the study. In conclusion, the study revealed that the activation of monocytes and blood platelets is accompanied by the elevation of monocytic TF expression in advanced liver cirrhosis. The monocytic TF is a significant link connecting clotting processes and inflammatory and immunological phenomena in liver cirrhosis.

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Blood platelet and monocyte activations and relation to stages of liver cirrhosis

Cited by 36 publications

References 30 publications

Relationship between vitamin D and IL-23, IL-17 and macrophage chemoattractant protein-1 as markers of fibrosis in hepatitis C virus Egyptians

Relationship between vitamin D and IL-23, IL-17 and macrophage chemoattractant protein-1 as markers of fibrosis in hepatitis C virus Egyptians

Concomitant presence of Aspergillus fumigatus and Stenotrophomonas maltophilia in the respiratory tract: a new risk for patients with liver disease?

Increase in expression of monocytic tissue factor (CD142) with monocytes and blood platelet activation in liver cirrhosis

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