Blood-pressure lowering efficacy of winged bean seed hydrolysate in spontaneously hypertensive rats, peptide characterization and a toxicity study in Sprague-Dawley rats

Abstract

Winged bean seed (WBS) is an underutilized tropical crop. The current study evaluates its potential to reduce blood pressure (BP) in spontaneously hypertensive rats and finds that it reduces BP significantly, in a dose-dependent manner. Five peptides with the sequences, RGVFPCLK, TQLDLPTQ, EPALVP, MRSVVT and DMKP, have been characterized in terms of their stability against ACE via in vitro and in silico modelling. All peptides exhibited IC values between 0.019 and 6.885 mM and various inhibitory modes, including substrate, prodrug and true inhibitor modes. The toxicity status of non-Current Good Manufacturing Practice (non-CGMP) peptides is evaluated and the results show that such peptides are toxic, and thus are not suitable to be tested in animals, particularly in repeated-dose studies. In short, WBS hydrolysate demonstrated in vitro ACE inhibitory properties and in vivo blood pressure lowering efficacy in rat models, fostering its potential as a functional food ingredient. Non-CGMP grade peptides are toxic and unfit for testing in animal models.

Identification, structure-activity relationship and in silico molecular docking analyses of five novel angiotensin I-converting enzyme (ACE)-inhibitory peptides from stone fish (Actinopyga lecanora) hydrolysates

“…The tendency of a particular peptide (ligand) to bind the protein receptor (ACE) at a specific conformation is rated by the GlideXP according to a rigid receptor approximation approach. Accordingly, the ligand-protein (peptide-ACE) fitting is governed by the potential to form hydrogen bonds, electrostatic interactions and to attain an acceptable root mean square deviation in comparison to the native complex [48–50].…”

“…We attributed the higher binding energy scores obtained for the peptides in this study to the effect of rigid-receptor approach used for calculation by the Glide program which deter good score for peptides with significant steric clashes in relation to its specified receptor conformation. However, in real situation the peptides may effectively bind with alternative conformation of the same ACE receptor protein [48, 50].…”

Identification, structure-activity relationship andin silicomolecular docking analyses of five novel angiotensin I-converting enzyme (ACE)-inhibitory peptides from stone fish hydrolysates

“…The tendency of a particular peptide (ligand) to bind the protein receptor (ACE) at a specific conformation is rated by the GlideXP according to a rigid receptor approximation approach. Accordingly, the ligand-protein (peptide-ACE) fitting is governed by the potential to form hydrogen bonds, electrostatic interactions and to attain an acceptable root mean square deviation in comparison to the native complex (46, 48).…”

“…We attributed the higher binding energy scores obtained for the peptides in this study to the effect of rigid-receptor approach used for calculation by the Glide program which deter good score for peptides with significant steric clashes in relation to its specified receptor conformation. However, in real situation the peptides may effectively bind with alternative conformation of the same ACE receptor protein (46, 48).…”

Pengaruh Perbedaan Waktu Panen Terhadap Karakteristik Kimia Biji Kecipir

“…Wahyuni (2010) melaporkan bahwa total isofalvon pada biji kecipir lebih tinggi dibandingkan dengan kedelai dan terdiri dari factor-2isoflavone, daidzein, glysiteine dan genisteine. Beberapa penelitian yang sudah ada mengenai biji kecipir berada pada ekplorasi dan identifikasi komponen gizi utama seperti pada lemak (Mohanty et al, 2013) dan peptida biji kecipir (Yea et al, 2018). Penelitian lainnya menunjukkan aplikasi biji kecipir sebagai sumber protein pengganti kedelai seperti pada tahu, susu dan yoghurt (Budijanto et al, 2011;Wijaya et al, 2015) dan aplikasi pada produk seperti dalam pembuatan margarin (Makeri et al, 2019).…”