Bloodstain Grouping—Elution v. Inhibition

Outteridge, R. A.

doi:10.1016/s0015-7368(63)70163-0

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Yamakami discovered the presence of A and B antigens in saliva [6] . In 1963, Outride compared the absorption elution and absorption inhibition method and concluded that the former is more sensitive [7] and the studies done by Kaur et al, [8] and Aye et al, [9] were in accordance to this. So in this study absorption elution method is followed in identification of ABO blood grouping in saliva.…”

Section: Discussionmentioning

confidence: 86%

Saliva in identification of ABO Blood Grouping Rhesus Factor

Harshada R

2020

Indian Journal of Forensic Medicine & Toxicology

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Aim: To assess the efficacy and reliability of saliva in identification of ABO blood grouping. Materials and Method:The study was conducted in the outpatient department of Oral Medicine and Radiology. The serum and saliva of 50 participants were collected and the blood group of the individual from serum was identified by the slide agglutination method and from saliva by the absoption elution method.Results: Data obtained were subjected to statistical analysis using Paerson's chi square test and it showed high significance (P = <0.001). Conclusion:The outcome of the present study showed that saliva can be used as an aid in forensics for identification of ABO blood grouping of an individual.

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Section: Discussionmentioning

confidence: 86%

Saliva in identification of ABO Blood Grouping Rhesus Factor

Harshada R

2020

Indian Journal of Forensic Medicine & Toxicology

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“…The reason was that it was much sensitive than absorption inhibition method. [8] Aye in 1977 conducted a similar study comparing the two methods but on sweat. In this study, too, more positive results were obtained by absorption elution method when compared to absorption inhibition method.…”

Section: Discussionmentioning

confidence: 99%

A comparison of absorption inhibition and absorption elution methods for estimation of ABO blood groups in saliva

Sen¹,

Vanishree²,

Hunasgi³

et al. 2015

JMRPS

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Background: ABO blood groups are the primary, most common, conspicuous, and easily detectable groups. These blood group specific antigens are not the exclusive domain of the erythrocyte, but abundantly present in many other bodily secretions such as sweat, semen and even saliva. Absorption inhibition and absorption elution are the main methods to detect salivary blood group antigens. The aim of this study is to detect the secretor status of 100 samples of saliva and to compare the diagnostic efficacy of absorption elution method and absorption inhibition methods in detecting the ABO (H) blood group antigens in secretors. Materials and Methods: Totally 100 un-stimulated whole saliva samples were analyzed for the presence of ABO (H) blood group specific antigens by absorption inhibition and absorption elution methods. Initially, secretor status was ascertained by detecting the H antigen in saliva, and both methods were used to detect the blood groups from saliva and the results were compared with that of blood which were statistically analyzed. Results: Of the 100 samples, 77 secretors and 23 non-secretors. Using absorption inhibition method 62 out of 77 samples gave results that matched with blood. Using absorption elution 70 out of 77 samples gave matching results. Absorption elution depicted better sensitivity, more true positive matches with blood as well as a higher Kappa coefficient. Conclusion: Absorption elution gave better results than absorption inhibition.

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“…The principle of the AE test involves the absorption of blood group-specific agglutinin to the surface of a substance having blood group agglutinogens, followed by the elution of the absorbed antibody under a high temperature, and the agglutination of the blood cells against the corresponding antigens. Cold agglutination increases the agglutination intensity [7][8][9][10][11][12][13][14][15][16][17][18]. In this study, our objective was to determine the ABO blood group from both the hard and soft tissue of the tooth by using the AE technique and also to ascertain if there are any variations in the identification of the blood groups from the teeth based on age and gender.…”

Section: Introductionmentioning

confidence: 99%

Detection of ABO Blood Groups From Dentin and Pulp by Using the Absorption-Elution Technique: A Forensic Cross-Sectional Study Among the Population of the Al Jouf Province, Saudi Arabia

Rao,

Alrayes,

Faisal Alshammari

et al. 2024

Cureus

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Background and objective Human teeth have a significant forensic importance. As they are the hardest of all human tissues, they are not just chemically stable but also their characteristics are maintained for a long time after death even in the most harsh environmental conditions. Despite the advances made in DNA analysis, fingerprinting, etc., ABO blood grouping still plays a significant role in the forensic practice in the field of personal identification, paternity disputes, and several other scenarios including the identification of mass disaster victims . The term blood groups refers to inherited antigens on the surface of red blood cells (RBCs) detected by specific antibodies. Since tooth pulp contains numerous blood vessels, blood group antigens are most certainly bound to be present in tooth pulp. Various studies have shown that blood group antigens in the pulp and dentin are preserved as long as up to two years after the demise of an individual. Absorption-elution technique has been proven to be the most sensitive, reliable, and consistent method to determine the ABO blood group from both the pulp and dentine. This study aimed to ascertain the ABO blood group from both the hard (dentin) as well as the soft tissue (pulp) of the tooth by using the absorption-elution (AE) technique and also to determine if there are any variations in identifying the blood groups from the teeth based on age and gender. Material and methods After obtaining due consent, we included patients of both genders aged between 16-60 years visiting the outpatient department (OPD) clinics at the College of Dentistry for periodontal or orthodontic extractions. One patient's blood type was determined by using the slide agglutination technique before any capillary blood extraction was performed; this patient served as a control. For this investigation, we used the pulp and powdered dentin samples taken from the dental extractions to test for the presence of ABO and Rhesus (Rh) factor antigens by using the AE method. The study samples were compared with the control for blood group determination. Statistical analysis was carried out using the chi-square test with Monte Carlo (MC) simulation to check for any correlation of blood grouping with age and gender. Results The dentin and pulp were shown to have positive blood group antigens for the ABO and Rh factors. While neither pulp nor dentin performed significantly differently in identifying the blood group antigens, pulp showed marginally higher accuracy. There was no discernible difference regarding gender or age in the dentin or pulp of any of the 45 samples studied. Conclusions For determining an individual's blood type and Rh factor, both the hard (dentin) and soft (pulp) tissues of a tooth are valid sources. This is particularly helpful in forensic medicine cases where teeth are the only remains that can be viably used to find out a person's identity.

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Bloodstain Grouping—Elution v. Inhibition

Cited by 4 publications

References 6 publications

Saliva in identification of ABO Blood Grouping Rhesus Factor

Saliva in identification of ABO Blood Grouping Rhesus Factor

A comparison of absorption inhibition and absorption elution methods for estimation of ABO blood groups in saliva

Detection of ABO Blood Groups From Dentin and Pulp by Using the Absorption-Elution Technique: A Forensic Cross-Sectional Study Among the Population of the Al Jouf Province, Saudi Arabia

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