BMSCs promote the differentiation of NSCs into oligodendrocytes via mediating Id2 and Olig expression through BMP/Smad signaling pathway

Song, Peiwen; Xiang, Xia; Han, Tianyu; Huang, Fang; Wang, Ying; Dong, Fulong; Zhang, Renjie; Ge, Peng; Shen, Cailiang

doi:10.1042/bsr20180303

Cited by 18 publications

(22 citation statements)

References 58 publications

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“…In the process of functional reconstruction aer SCI, neurons subsequently differentiate into functional neurons and play a role in signal transmission, whereas oligodendrocytes promote the regeneration of the myelin sheath and axons, which exert a positive effect on functional recovery. 45,46 The results of cell differentiation assays were consistent with the expected results for spinal cord recovery, suggesting that PLGA/FTY720 exerted a positive effect on functional reconstruction. Although qPCR and immunouorescence staining did not reveal obvious effects of PLGA/FTY720 on NSC differentiation into neurons, the proper mass ratio of PLGA/FTY720 nanobres induced NSC differentiation into oligodendrocytes, which promote axon regeneration.…”

Section: Discussionsupporting

confidence: 80%

Local delivery of FTY720 and NSCs on electrospun PLGA scaffolds improves functional recovery after spinal cord injury

Kong

Xia

et al. 2019

RSC Adv.

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Section: Discussionsupporting

confidence: 80%

Local delivery of FTY720 and NSCs on electrospun PLGA scaffolds improves functional recovery after spinal cord injury

Kong

Xia

et al. 2019

RSC Adv.

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“…Neural stem cells (NSCs) existing in the subventricle zone of the brain's germinal region and the subgranular zone of the hippocampus are capable of self-renewing, proliferating, migrating, and differentiating into various types of cells within the brain and spinal cord tissue [13,41]. NSC differentiation into neurons, astrocytes, and oligodendrocytes could replace necrotic cells resulting from injuries to promote the structural and functional repair of the brain and spinal cord [50].…”

Section: Introductionmentioning

confidence: 99%

DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

et al. 2020

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Background: The limited neuronal differentiation of the endogenous or grafted neural stem cells (NSCs) after brain injury hampers the clinic usage of NSCs. Panax notoginseng saponins (PNS) were extensively used for their clinical value, such as in controlling blood pressure, blood glucose, and inhibiting neuronal apoptosis and enhancing neuronal protection, but whether or not it exerts an effect in promoting neuronal differentiation of the endogenous NSCs is completely unclear and the potential underlying mechanism requires further exploration. Methods: Firstly, we determined whether PNS could successfully induce NSCs to differentiate to neurons under the serum condition. Mass spectrometry and quantitative polymerase chain reaction (Q-PCR) were then performed to screen the differentially expressed proteins (genes) between the PNS + serum and serum control group, upon which dihydropyrimidinase-like 2 (DPYSL2), a possible candidate, was then selected for the subsequent research. To further investigate the actual role of DPYSL2 in the NSC differentiation, DPYSL2-expressing lentivirus was employed to obtain DPYSL2 overexpression in NSCs. DPYSL2-knockout rats were constructed to study its effects on hippocampal neural stem cells. Immunofluorescent staining was performed to identify the differentiation direction of NSCs after 7 days from DPYSL2 transfection, as well as those from DPYSL2-knockout rats. Results: Seven differentially expressed protein spots were detected by PD Quest, and DPYSL2 was found as one of the key factors of NSC differentiation in a PNS-treated condition. The results of immunostaining further showed that mainly Tuj1 and GFAP-positive cells increased in the DPYSL2-overexpressed group, while both were depressed in the hippocampal NSCs in the DPYSL2-knockout rat. Conclusions: The present study revealed that the differentiation direction of NSCs could be enhanced through PNS administration, and the DPYSL2 is a key regulator in promoting NSC differentiation. These results not only emphasized the effect of PNS but also indicated DPYSL2 could be a novel target to enhance the NSC differentiation in future clinical trials.

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“…NSCs were cultured as described in our previous studies [27,28]. Cells were obtained from the subventricular zone of SD rats.…”

Section: Cultivation Differentiation and Transfection Of Nscsmentioning

confidence: 99%

“…MSCs were cultured following the procedure described in our previous studies [27,28]. The cells were isolated from the bone marrow of Fischer 344 rats and were cultured in DMEM (low glucose, Hyclone) containing 10% fetal bovine serum (FBS) (Gibco, USA) and 1% antibiotic solution at a density of 1 × 10 6 cells/cm 2 .…”

Section: Mesenchymal Stem Cell Culture and The Preparation Of Conditimentioning

confidence: 99%

BMSC-CM prevents over-differentiation of NSCs to astrocytes by upregulating Smad 6 expression

Han

Song

Xia

et al. 2020

Preprint

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Background: Mesenchymal stem cells (MSCs) are a promising therapy for spinal cord injury (SCI) as they can provide a favorable environment for the regrowth of neurons and axons by inhibiting receptor-regulated Smads (R-Smads) in endogenous neural stem cells (NSCs). However, their mechanism of action and effect on the expression of inhibitory Smads (I-Smads) remains unclear.Method: Conditioned medium (CM) was collected from bone marrow MSCs (BMSCs) isolated from rats with SCIs, and its effect on the regulation of Smad 6 expression was tested in vitro (in NSCs) and in vivo (SCI rats). Western blot analysis and immunohistochemistry staining were used to investigate the proportion of neurons and astrocytes in vitro and in vivo. BBB scores were used to assess the neurological outcome of SCI rats at different time points.Results: BMSC-CM could upregulate Smad 6 expression in vitro. BMSC-CM-induced upregulation was suppressed by pre-treatment with the TGF-β type I receptor kinase inhibitor SB431542. BMSC-CM was able to promote the differentiation of NSCs to neurons; Smad 6 knockdown in NSCs partly weakened this effect on neural differentiation. In vivo, Smad 6 expression in the later phase of injury was closely associated with BMSC-CM treatment. Conclusion: BMSC-CM can upregulate Smad 6 expression by the secretion of TGF-β. It promotes the differentiation of NSCs into neurons, partly through upregulation of Smad 6.

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BMSCs promote the differentiation of NSCs into oligodendrocytes via mediating Id2 and Olig expression through BMP/Smad signaling pathway

Cited by 18 publications

References 58 publications

Local delivery of FTY720 and NSCs on electrospun PLGA scaffolds improves functional recovery after spinal cord injury

Local delivery of FTY720 and NSCs on electrospun PLGA scaffolds improves functional recovery after spinal cord injury

DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

BMSC-CM prevents over-differentiation of NSCs to astrocytes by upregulating Smad 6 expression

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