Bombella favorum sp. nov. and Bombella mellum sp. nov., two novel species isolated from the honeycombs of Apis mellifera

Hilgarth, Maik; Redwitz, Johannes; Ehrmann, Matthias A.; Vogel, Rudi F.; Jakob, Frank

doi:10.1099/ijsem.0.004633

Cited by 22 publications

(15 citation statements)

References 43 publications

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“…Bombella spp. are described to grow in media containing 50% of glucose [ 13 ], a trait likely linked to the sugar-rich habitat as a honeybee symbiont. We compared the maximal growth rate of available Bombella strains with G. oxydans DSM46615 at 100 and 300 g L −1 in order to illustrate the high osmotolerance of Bombella spp.…”

Section: Discussionmentioning

confidence: 99%

“…Strains were isolated from Apis mellifera honeycombs in Freising (Germany) and from Apis mellifera honeycombs in Salzburg (Austria). Isolation was performed as described in a previous study by Hilgarth et al [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…A member of this group is the acetic acid bacteria Bombella (previously also invalidly designated to as Parasaccharibacter or “Alpha 2.2”), a genus composed of four described species, namely B. apis , B . intestini , B. favorum and B. mellum [ 11 , 12 , 13 , 14 ]. Focus has been drawn to this genus, since it was shown to have a positive impact against fungal pathogens [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Härer¹,

Hilgarth²,

Ehrmann³

2022

Microorganisms

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It is known that the bacterial microbiota in beehives is essential for keeping bees healthy. Acetic acid bacteria of the genus Bombella colonize several niches in beehives and are associated with larvae protection against microbial pathogens. We have analyzed the genomes of 22 Bombella strains of different species isolated in eight different countries for taxonomic affiliation, central metabolism, prophages, bacteriocins and tetracycline resistance to further elucidate the symbiotic lifestyle and to identify typical traits of acetic acid bacteria. The genomes can be assigned to four different species. Three genomes show ANIb values and DDH values below species demarcation values to any validly described species, which identifies them as two potentially new species. All Bombella spp. lack genes in the Embden–Meyerhof–Parnas pathway and the tricarboxylic acid cycle, indicating a focus of intracellular carbohydrate metabolism on the pentose phosphate pathway or the Entner–Doudoroff pathway for which all genes were identified within the genomes. Five membrane-bound dehydrogenases were identified that catalyze oxidative fermentation reactions in the periplasm, yielding oxidative energy. Several complete prophages, but no bacteriocins, were identified. Resistance to tetracycline, used to prevent bacterial infections in beehives, was only found in Bombella apis MRM1T. Bombella strains exhibit increased osmotolerance in high glucose concentrations compared to Gluconobacter oxydans, indicating adaption to high sugar environments such as beehives.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Härer¹,

Hilgarth²,

Ehrmann³

2022

Microorganisms

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“…Li et al first proposed the genus Bombella as a member of the Alphaproteobacteria (49), with the description of Bombella intestine as the type species from bumble bee crop. At the time of this writing, the genus Bombella has just four validly named species (49)(50)(51). Interestingly, these Bombella spp.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota analysis of the western honeybee (Apis mellifera L.) infested with the mite Varroa destructor reveals altered bacterial and archaeal community

Kim

Park

2022

Preprint

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The western honeybee, Apis mellifera L., is a crop pollinator that makes royal jelly and other hive products. However, widespread concerns arise about opportunistic diseases (e.g., bacteria, fungi, or mites) or chemicals that have an effect on the health and number of colonies, as well as their activity. The relationships between the gut microbiota and its host are currently being researched extensively. The effects of Varroa destructor infection on the gut microbial community, in particular, have received little investigation. This work utilized amplicon sequencing of the bacterial and archaeal 16S rRNA genes to assess the bacterial and archaeal communities of adult bee groups (healthy and affected by Varroa designed in NG and VG, respectively) and larvae from Varroa destructor-infected hives. Our results suggest that the genus Bombella was substantially dominant in larvae, while the genera Gillamella, unidentified Lactobacillaceae, and Snodgrassella were significantly dominant in adult bees. NG and VG, on the other hand, did not differ statistically significantly. The PICRUSt study revealed a significant difference in the KEGG classifications of larvae and adult bee groups. A greater number of genes involved in cofactor and vitamin production were identified in larvae. Additionally, despite the complexity of the honeybee’s bacterial community, all groups exhibited a straightforward archaeal community structure. Surprisingly, methanogen was detected in low abundance in the microbiota of honeybees. In summary, larvae and adult bees infected with Varroa destructor exhibit altered gut microbiota composition and function.

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“…Less dense species than Proteobacteria are; Commensalibacter sp. (Alpha 2.1) and Bartonella apis (Alpha 1); Parasaccharibacter apium, Bombella apis , Bombella mellum, Bombella favorum (Acetobacteraceae family, Alpha 2.2), Apibacter adventoris, Apibacter mensalis and Frischella perrara (Orbaceae family) (Kešnerová, Moritz, & Engel, 2016;Kwong & Moran, 2016b;Jones et al, 2017;Kwong, Steele, & Moran, 2018;Bleau et al, 2020;Dong et al, 2020;Tola et al, 2020;Hilgarth, Redwitz, Ehrmann, Vogel, & Jakob, 2021). Four Lactobacillus species, 2 Gilliamella species, 1 Bifidobacterium species and 1 Snodgrassella species, which constitute the intestinal core microbiome of honey bees, were determined.…”

Section: Characteristicsmentioning

confidence: 99%

Microbiota and Its Importance in Honey Bees

Borum¹

2021

BSTD

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In recent years, research on human and animal health has emerged as a very important microbiota and microbiome. Microbiota; has important functions in metabolism, immune system, growth and development. In recent years, it has been understood that the microbiota is effective in the protection of bee health in colony so prevent losses in honey bees. Season, flora, food sources, age of the individual, duties in the hive, chemicals used in the fight against parasites and pathogens, and many other factors can be effective on the microbiome of honey bees.

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Bombella favorum sp. nov. and Bombella mellum sp. nov., two novel species isolated from the honeycombs of Apis mellifera

Cited by 22 publications

References 43 publications

Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Gut microbiota analysis of the western honeybee (Apis mellifera L.) infested with the mite Varroa destructor reveals altered bacterial and archaeal community

Microbiota and Its Importance in Honey Bees

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