Bone marrow-derived mesenchymal cells feature selective migration behavior on submicro- and nano-dimensional multi-patterned substrates

Klymov, Alexey; Bronkhorst, Ewald M.; Riet, Joost te; Jansen, John A.; Walboomers, X. Frank

doi:10.1016/j.actbio.2015.01.016

Cited by 26 publications

(20 citation statements)

References 21 publications

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“…S3 ). Additionally, it was found that cell adhesion was sensitive to the topographic gradients, resulting in a cell density gradient along the substrate with 0−1.0cm 50S grad and 0−1.0cm 150 S grad probably due to variations in cell adhesion capabilities and cell migration 32 . Cells are able to sense a gradient and can make a migration directional response (haptotaxis), although this was not included in the investigations here.…”

Section: Resultsmentioning

confidence: 99%

Directional nanotopographic gradients: a high-throughput screening platform for cell contact guidance

Zhou

Kühn

Huisman

et al. 2015

Sci Rep

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A novel approach was developed using PDMS-substrates with surface-aligned nanotopography gradients, varying unidirectional in amplitude and wavelength, for studying cell behavior with regard to adhesion and alignment. The gradients target more surface feature parameters simultaneously and provide more information with fewer experiments and are therefore vastly superior with respect to individual topography substrates. Cellular adhesion experiments on non-gradient aligned nanowrinkled surfaces displayed a linear relationship of osteoblast cell adhesion with respect to topography aspect ratio. Additionally, an aspect ratio of 0.25 was found to be most efficient for cell alignment. Modification of the surface preparation method allowed us to develop an approach for creating surface nanotopography gradients which innovatively provided a superior data collection with fewer experiments showing that 1) low amplitude with small wavenumber is best for osteoblast cell adhesion 2) indeed higher aspect ratios are favorable for alignment however only with features between 80–180 nm in amplitude and 450–750 nm in wavelength with a clear transition between adhesion and alignment efficiency and 3) disproved a linear relationship of cell adhesion towards aspect ratio as was found for single feature substrate analysis.

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Section: Resultsmentioning

confidence: 99%

Directional nanotopographic gradients: a high-throughput screening platform for cell contact guidance

Zhou

Kühn

Huisman

et al. 2015

Sci Rep

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“…Comparing micro to nanopattern designs in recent research suggests favorability towards the latter [78], yet there are studies that try to implement both these patterns into a single gradient mold [79, 80]. As previously mentioned, nanotopography does well at aligning cells along its specific pattern, more so than microtopography.…”

Section: Biophysical Regulation Of Cell Reprogrammingmentioning

confidence: 99%

“…Gradient biomaterials can be used to manipulate cell behavior towards niches that actively represent specific ECM properties for reprogramming and differentiation. Previously mentioned topography [79, 80], stiffness [95], and strain [107] gradient biomaterials have shown that cells can be controlled using these active niches for reprogramming purposes.…”

Section: Biophysical Regulation Of Cell Reprogrammingmentioning

confidence: 99%

A biomaterial approach to cell reprogramming and differentiation

Long

Kim

et al. 2017

J. Mater. Chem. B

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Cell reprogramming of somatic cells into pluripotent states and subsequent differentiation into certain phenotypes has helped progress regenerative medicine research and other medical applications. Recent research has used viral vectors to induce this reprogramming; however, limitations include low efficiency and safety concerns. In this review, we discuss how biomaterial methods offer potential avenues for either increasing viability and downstream applicability of viral methods, or providing a safer alternative. The use of non-viral delivery systems, such as electroporation, micro/nanoparticles, nucleic acids and the modulation of culture substrate topography and stiffness have generated valuable insights regarding cell reprogramming.

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“…Among the required characteristics of a biomaterial, surface texturing has a strong influence on biocompatibility, adhesion, and cell growth results. Surface texturing treatments thus aim to achieve a better tissue-implant interaction 7 .…”

Section: Introductionmentioning

confidence: 99%

Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces

et al. 2018

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Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples and two types of electropolished samples that were treated for 4 min and 12 min, respectively. The systems were characterized by atomic force microscopy, contact profilometry, X-ray diffraction, and wettability. Each system was submitted to a cell culture containing hESCs-MP cells for 14 days, and the resultant cell growth on each system was then evaluated. The results indicated that surfaces with higher nanometric and micrometric roughnesses experienced greater hESCs-MP cell growth in osteogenic media. The same behavior was not observed for cell growth in non-osteogenic media due to the absence of dexamethasone, which is responsible for controlling protein adsorption on the surface.

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Bone marrow-derived mesenchymal cells feature selective migration behavior on submicro- and nano-dimensional multi-patterned substrates

Cited by 26 publications

References 21 publications

Directional nanotopographic gradients: a high-throughput screening platform for cell contact guidance

Directional nanotopographic gradients: a high-throughput screening platform for cell contact guidance

A biomaterial approach to cell reprogramming and differentiation

Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces

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