Bone mineral density and composition in rat pregnancy: effects of streptozotocin‐induced diabetes mellitus and insulin replacement

Tein,; Breen, SA; Loveday, BE; Devlin, Henry B.; Balment, R. J.; Boyd, RD; Sibley, CP; Garland, HO

doi:10.1113/expphysiol.1998.sp004099

Cited by 15 publications

(15 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present finding of reduced bone size and thereby reduced Ca content, but with maintained Ca concentration, corroborates previous reports of diabetes affecting bone development (Verhaeghe et al 1986, Tein et al 1998. The higher ratios of hypertrophic cells to proliferative cells in the growth plate found in the diabetic rat femurs in this study may be explained by a reduced local synthesis of mitogenic growth factors, such as insulinlike growth factor-I (Lazowski et al 1994).…”

Section: Figuresupporting

confidence: 81%

gamma-Linoleic acid and ascorbic acid ameliorate the effects of experimental diabetes on electrolyte and bone homeostasis in pregnant rats

Braddock¹,

Simán²,

Hamilton³

et al. 2002

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Experimental diabetes in rats is associated with excessive electrolyte loss in the urine, which is further accentuated by pregnancy, particularly of Ca. Supplementation with essential fatty acids and antioxidants has proven beneficial in treating several types of complications, including nephropathy. The present study investigated the effect of -linoleic acid (GLA; 500 mg/kg per day; group DG) and ascorbate (290 mg/kg per day; group DA), alone and in combination (group DGA), as well as ascorbyl-GLA (790 mg/kg per day; group DASG), on urinary electrolyte output and skeletal composition in pregnant streptozotocin-diabetic rats. Urine was collected in metabolism cages before and throughout pregnancy. Diabetic rats (DP) increased their urine volume as compared with control (CP) throughout the experiment, reaching an output of more than 13 times that of the control group by the end of pregnancy (CP 24 4, DP 316 21, DG 223 21, DA 221 14, DASG 163 17, DGA 220 19 ml urine/24 h). Concomitant with increased urine volume was a reduction of urinary Na (CP 47 14, DP 22 5 mmol/l), K (CP 210 34, DP 31 1 mmol/l) and Mg (CP 14 1, DP 3·8 0·2 mmol/l) concentration, but not of Ca concentration (CP 5·4 1·5, DP 6·3 0·6 mmol/l), and hence total Ca loss was relatively most severe. All the treatments reduced urine volume with no effects on electrolyte concentration as compared with DP, with no significant difference between the treatments. A reduced bone size and bone Ca content was partially ameliorated by the diet supplementation. We have concluded that GLA and ascorbate, alone or in combination, prevent urinary electrolyte loss in pregnant rats and do so by reducing urine production.

show abstract

Section: Figuresupporting

confidence: 81%

gamma-Linoleic acid and ascorbic acid ameliorate the effects of experimental diabetes on electrolyte and bone homeostasis in pregnant rats

Braddock¹,

Simán²,

Hamilton³

et al. 2002

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some animal experiments revealed that low content of Ca, P, and Mg associated with the BMD decrease in diabetic osteoporosis [21,22], but it is not clear how the bone mineral element changes after insulin treatment. The aim of this study is to assess the correlation between the BMD and the mineral content after insulin treatment in STZ-induced diabetic rats.…”

Section: Introductionmentioning

confidence: 99%

Reversal of Osteoporotic Changes of Mineral Composition in Femurs of Diabetic Rats by Insulin

Zhang

Fei

Zhang

et al. 2007

Biol Trace Elem Res

View full text Add to dashboard Cite

Insulin plays an important role in bone prevention of diabetic osteoporosis, but little is known about the relation between the bone mineral density (BMD) increase and the change of mineral element content after treated with insulin. To address this problem, male Wistar rats were randomly divided into three groups: normal group (n = 6), streptozotocin-induced diabetic group (n = 5), and streptozotocin-induced diabetic group with insulin treatment (n = 5). The femoral BMD was measured by dual energy X-ray absorptiometry, and the element content was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The results showed that the femoral BMD in diabetic group was significantly lower than that in normal group (P < 0.01) but restored by insulin treatment (P < 0.01 vs diabetic group). ICP-AES analysis revealed that the element content of calcium (Ca), phosphorous (P), magnesium (Mg), strontium (Sr), and potassium (K) in diabetic group were remarkably lower than those in normal group (P < 0.01) but only Ca, P, and Mg content were significantly increased compared with diabetic group (P < 0.05) after insulin treatment. However, no significant differences were observed in element zinc (Zn) content among three groups. Our findings suggested that the loss of Ca, P, Mg, Sr, and K content accounted for the lower BMD in streptozotocin-induced diabetes rats, insulin treatment could restore BMD by increasing the content of Ca, P, and Mg.

show abstract

“…The occurrence of different abnormalities indicating altered bone formation after inducing DM with streptozotocin (STZ) is well documented (Giglio and Lama, 2001;Hough et al, 1981;Tein et al, 1998). Streptozotocin-induced diabetes mellitus (STZ-DM), caused by the destruction of pancreatic β-cells and is similar to T1DM in human, is characterized by mild to moderate hyperglycemia, glucosuria, polyphagia, hypoinsulinemia, hyperlipidemia, and weight loss.…”

Section: Diabetic Modelmentioning

confidence: 99%

“…The rats in the control group were injected intraperitoneal with a single dose of 0.1M sodium citrate buffer (pH 4.5), while the rats in the DM group were injected intra-peritoneal with a single dose of citrate buffer containing 60mg/kg body weight of streptozotocin (Sigma Chemical Co., St. Louis, MO, USA). (Alkan et al, 2002;McCracken et al, 2006;Shyng et al, 2001;Tein et al, 1998) All animals were fed on standard Rodent diet (Rodent Diet CE-2; Japan Clea Inc., Shizuoka, Japan) with free access to water. Body weights, the presence of glucose in urine and blood glucose levels were recorded on day 0, 2, 7, 14, 21 and 28 after STZ injection.…”

Section: Inducing Diabetic Conditionmentioning

confidence: 99%

The Effect of Type 1 Diabetes Mellitus on the Craniofacial Complex

Abbassy¹,

Watari²,

Ono³

2011

Type 1 Diabetes Complications

View full text Add to dashboard Cite

Bone mineral density and composition in rat pregnancy: effects of streptozotocin‐induced diabetes mellitus and insulin replacement

Cited by 15 publications

References 23 publications

gamma-Linoleic acid and ascorbic acid ameliorate the effects of experimental diabetes on electrolyte and bone homeostasis in pregnant rats

gamma-Linoleic acid and ascorbic acid ameliorate the effects of experimental diabetes on electrolyte and bone homeostasis in pregnant rats

Reversal of Osteoporotic Changes of Mineral Composition in Femurs of Diabetic Rats by Insulin

The Effect of Type 1 Diabetes Mellitus on the Craniofacial Complex

Contact Info

Product

Resources

About