Bone Regeneration Effect of Hyperbaric Oxygen Therapy Duration on Calvarial Defects in Irradiated Rats

Abstract

Objective. In this study, we evaluated changes in bone remodeling in an irradiated rat calvarial defect model according to duration of hyperbaric oxygen therapy. Materials and Methods. The 28 rats were divided into four groups. Radiation of 12 Gy was applied to the skull, and 5-mm critical size defects were formed on both sides of the skull. Bone grafts were applied to one side of formed defects. From the day after surgery, HBO was applied for 0, 1, and 3 weeks. At 6 weeks after bone graft, experimental sites were removed and analyzed for radiography, histology, and histomorphometry. Results. Micro-CT analysis showed a significant increase in new bone volume in the HBO-3 group, with or without bone graft. When bone grafting was performed, BV, BS, and BS/TV all significantly increased. Histomorphometric analysis showed significant increases in %NBA and %BVN in the HBO-1 and HBO-3 groups, regardless of bone graft. Conclusion. Hyperbaric oxygen therapy was effective for bone regeneration with only 1 week of treatment.

Hyperbaric Oxygen Therapy and A-PRF Pre-Treated Implants in Severe Periodontitis: A Case Report

“…Treating osteoblasts with HBOT also improved their proliferation rate compared to hypoxic or normoxic conditions and increased alkaline phosphatase activity (ALP) [ 25 ]. Hyperbaric oxygen relieved radiation-induced osteonecrosis in rats by contrasting the inflamed, hypocellular, hypovascular, and hypoxic environment in the damaged bone and promoting vascularization [ 13 , 14 ]. In patients with AVNFH, hyperbaric oxygen was demonstrated to reduce inflammatory markers such as tumor necrosis factor-α (TNF-α) and interleukin (IL)-6, creating a favorable osteogenic environment [ 8 ].…”

“…It has been successfully used in many pathologies characterized by osteonecrosis, such as avascular necrosis of the femoral head (AVNFH) and osteonecrosis of the knee [ 7 , 8 , 9 ]. In particular, referring to the oral and maxillofacial districts, HBOT increased the bone regeneration rate in osteoradionecrosis, mandibular osteomyelitis, and dental implants [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

“…Caused mainly by bacterial plaque and local immune response, periodontitis can also lead to loss of tooth attachment. During the disease’s progression, a change in the oral microbiota is recorded, showing a prevalence of anaerobic bacteria such as P. gingivalis [ 13 ]. HBOT in periodontitis was shown to improve clinical parameters such as probe depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP) compared with standard antibiotic and dental medicine treatments.…”

Stimulation of Distraction Osteogenesis in Limb Lengthening: Our Concept

“…Формирование новой кости напрямую зависит от снабжения тканей кислородом и значительно выше при нормоксических условиях [42]. Положительное влияние гБО на регенерацию тканей доказано экспериментально на животных и в клинической практике [43,44]. гБО кислородом стимулирует процессы остеогенеза при удлинении конечностей, ликвидирует ишемию, развивающуюся в тканях, повышает насыщение их кислородом, улучшает обменные процессы, о чем свидетельствует исчезновение отеков и болевых ощущений [45,46] [39,47,48,49,50,51].…”

Citric acid, but not tetracycline, improves the microscopic pattern of healing of particulate autogenous bone grafts in critical‐size defects

“…Several studies in which μCT was used to assess the repair of critical defects have shown that this method confirms and validates histomorphometric findings, besides adding relevant information such as bone mineral density, bone mineral content and bone volume. 26,38,39,[45][46][47][48][49][50] The inclusion of immunohistochemical analysis for bone resorption and neoformation markers in earlier periods of healing could also contribute to the understanding of the biological phenomena that may explain the behavior of demineralized autogenous bone as a graft material.…”