Bone-Seeking Albumin-Nanomedicine for In Vivo Imaging and Therapeutic Monitoring

Abstract

Malignant osteolysis associated with irreversible primary bone tumors and bone metastases remains a clinically urgent problem. Exploiting the imaging and therapy function of flexible nanomedicine can provide an alternative for therapeutic navigation and monitoring of malignant osteolysis. Here, we report the development of albumin-based gadolinium oxide nanoparticles loaded with doxorubicin and conjugated with bone-seeking alendronate for targeted delivery and therapeutic monitoring. Compared with nontargeted nanomedicine, bone-seeking accumulation and retention can be proven by MRI in a rat model of focal malignant osteolysis. Meanwhile, we observed a whole-body distribution in the consecutive SPECT imaging after radiolabeling with 125I, SPECT imaging also indicated the enhanced bone tumor accumulation and prolonged retention. Resulting from the high drug loading and 131I labeling efficiency, the targeted nanomedicine exhibited significant chemotherapy and inter-radiotherapy capacity. Ultimately, the tumor burden of rats was obviously decreased except for the nontargeted group and the empty carrier group. In vivo CT imaging and pathological analysis revealed that the combined therapy was an efficient measure for antiosteolysis. Our findings suggest that albumin-based nanomedicine can provide a platform for bone-seeking diagnosis and therapeutic monitoring.

Methods for Radiolabeling Nanoparticles (Part 3): Therapeutic Use

“…Chen et al developed albumin-based gadolinium-oxide NPs (GdNPs) loaded with doxorubicin (DOX) and conjugated with bone-seeking alendronate (ALN) to treat malignant bone destruction. The abundance of tyrosine residues in the albumin molecule resulted in a high radiolabeling efficiency [ 107 ].…”

Recent Advances in Nanotechnology-Based Diagnosis and Treatments of Human Osteosarcoma

“…SPECT/CT imaging has shown outstanding penetration capacity and is more applicable for deep tissue imaging and especially for OSA imaging [ 66 , 67 ]. A perfect option for the therapeutic management and evaluation of malignant osteolysis could be the imaging and therapy role of versatile nanomaterials [ 68 ]. Different forms of nanomedicines based on albumin have been investigated for cancer therapy, guided imaging, and biosensors [ 69 , 70 , 71 ].…”

“…For example, an alternative to therapeutic navigation and monitoring of malignant osteolysis could be to leverage the imaging and therapy role of flexible nanomedicine. Chen et al reported the development of albumin-based gadolinium oxide nanoparticles loaded with doxorubicin and conjugated with bone-seeking alendronate for targeted delivery and therapeutic monitoring [ 68 ]. After radio labeling with 125 I and SPECT imaging, the authors observed a good distribution of NPs in the body.…”

Metal-based nano-delivery platform for treating bone disease and regeneration

“…A few emerging technologies, including tissue engineering material transplantation ( Liu J. Y. et al, 2021 ), stem cell technology ( Shang et al, 2021 ), and nanomedicine ( Qiao K. et al, 2022 ), are potential means to promote the treatment of complex bone diseases. In particular, nanomedicine, based on various nanobiomaterials has significantly accelerated the diagnosis research, treatment, and regeneration of bone diseases, and has obtained numerous achievements ( Chen et al, 2020 ; Hagaman et al, 2021 ). There are a wide variety of nanobiomaterials, including carbon-based, metal-based, virus-based, lipid-based, polymer-based, liposomes, cubes, micelles, exosomes, and cell membrane coatings ( Sharma et al, 2021 ).…”