Bordetella bronchiseptica bateriophage suppresses B. bronchiseptica-induced inflammation in swine nasal turbinate cells

Park, Ga Young; Lee, Hye Min; Yu, Hyun Jin; Son, Jee Soo; Park, Sang Joon; Song, Kyoung Seob

doi:10.1007/s13258-018-0755-4

Cited by 5 publications

(6 citation statements)

References 20 publications

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“…First, we performed cell viability assays and confirmed that neither B. bronchiseptica nor Bor-BRP-1 exhibited cytotoxicity in a dose-or time-dependent manner (data not shown). B. bronchiseptica administration induced inflammatory cytokine secretion and mucin gene expression to control the respiratory microenvironment in our previous study (Park et al 2018). In addition, Bor-BRP-1 was challenged for 24 h in prior B. bronchiseptica infections.…”

Section: Bor-brp-1 Significantly Inhibited B Bronchiseptica-induced mentioning

confidence: 78%

“…Swine nasal turbinate (PT-K75) cells were purchased from ATCC (CRL-2528; Manassas, VA) and cultured as previously described (Park et al 2018). Cells were prepared at 5 × 10 5 cells/well in 6-well plates and incubated for approximately one day to reach full confluence.…”

Section: Methodsmentioning

confidence: 99%

“…According to a previous study, the inoculum of B. bronchiseptica to PT-K75 cells was selected at 1 × 10 7 cfu/ml. Bor-BRP-1 bacteriophage was produced based on a previous study (Park et al 2018). Bor-BRP-1 phages were applied 24 h prior to B. bronchiseptica-infection (1 × 10 7 cfu/ml) at 10 × concentration.…”

Section: Methodsmentioning

confidence: 99%

“…(Petrovic et al 2017;Prüller et al 2015;Biswas et al 2002;Dayao et al 2014;Niemann et al 2018). Previously, we reported that Bor-BRP-1 down-regulated B. bronchiseptica-induced inflammation (Park et al 2018). However, the physiological mechanism by which Bor-BRP-1 suppresses B. bronchiseptica-induced inflammation remains unidentified.…”

Section: Introductionmentioning

confidence: 92%

“…This impairs swine feeding and causes a reduction in growth performance resulting in great economic losses in the pig-rearing industry. 1 3 B. bronchiseptica is often isolated in mixtures with other bacterial or viral pathogens such as porcine reproductive and respiratory syndrome (PRRS) virus, porcine respiratory coronavirus, swine influenza virus (SIV), Haemophilus parasuis, Pasteurella multocida, and Streptococcus suis, from respiratory diseases in swine (Chen et al 2019;Park et al 2018;King et al 2002). B. bronchiseptica infection predisposes the upper respiratory tract to colonization with secondary pathogens including P. multocida and S. suis (Brockmeier 2004;Pósa et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Specific bacteriophage of Bordetella bronchiseptica regulates B. bronchiseptica-induced microRNA expression profiles to decrease inflammation in swine nasal turbinate cells

Park

Son

et al. 2020

Genes Genom

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Background Respiratory diseases in pigs are the main health concerns for swine producers. Similar to the diseases in human and other animals, respiratory diseases are primary related to morbidity and are the result of infection with bacteria, viruses, or both. B. bronchiseptica causes serious respiratory diseases in the swine airway track. However, the B. bronchisepticaspecific bacteriophage has diverse advantages such as decreasing antibiotic overuse and possible therapeutic potential against bacteria.Objective The objects of this study were to investigate the therapeutic effect of specific B. bronchiseptica bacteriophages and to identify genes related to bacteriophage signaling utilizing RNA microarrays in swine nasal turbinate cells. Methods Bor-BRP-1 phages were applied 24 h prior to B.bronchiseptica infection (1 × 10 7 cfu/ml) at several concentrations of bacterial infection. Cells were incubated to detect cytokines and 24 h to detect mucin production. And real-time quantitative PCR was performed to examine related genes expression. To determine the change of total gene expression based on B.bronchiseptica and Bor-BRP-1 treatment, we performed RNA sequencing experiments. Results The results showed that B. bronchiseptica induced increased expression of several inflammatory genes such as IL-1β, IL-6, and Muc1 in a dose-dependent manner. However, Bor-BRP-1 induced reduction of gene expression compared to the B. bronchiseptica induction group. In addition, microarrays detected Bor-BRP-1-altered inflammatory gene expression against B. bronchiseptica, reducing B. bronchiseptica-induced airway inflammation in swine epithelial cells. Conclusion These results suggest that the specific bacteriophage has a therapeutic potential to defend against B. bronchiseptica infection by altering inflammatory gene expression profiles. Song declare that we have no conflict of interest.Ethical approval This article does not contain any studies with human subjects or animals performed by any of the authors.

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Section: Bor-brp-1 Significantly Inhibited B Bronchiseptica-induced mentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%