Boron stabilizes peroxide mediated changes in the structure of heme proteins

Ali, Shakir; Farooqi, Humaira; Prasad, Ram; Naime, Mohammad; Routray, Indusmita; Yadav, Savita; Ahmad, Faizan

doi:10.1016/j.ijbiomac.2010.05.013

Cited by 17 publications

(6 citation statements)

References 25 publications

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“…Boron has an unusual chemistry which has been suggested to help stabilize the structures of various macromolecular complexes including the membrane and protein complexes [ 44 – 45 ]. As suggested by Hunt, boron may interact with one-to-four OH groups or a nitrogen group on specific biological ligands, such as the flavin/pyridine nucleotides and serine proteases.…”

Section: Discussionmentioning

confidence: 99%

Boron Induces Lymphocyte Proliferation and Modulates the Priming Effects of Lipopolysaccharide on Macrophages

Routray

Ali

2016

PLoS ONE

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Chemical mediators of inflammation (CMI) are important in host defense against infection. The reduced capacity of host to induce the secretion of these mediators following infection is one of the factors in host susceptibility to infection. Boron, which has been suggested for its role in infection, is reported in this study to increase lymphocyte proliferation and the secretion of CMI by the lipopolysaccharide (LPS)-stimulated peritoneal macrophages in BALB/c mice. Boron was administered to mice orally as borax at different doses for 10 consecutive days, followed by the stimulation of animals with ovalbumin and isolation of splenocytes for proliferation assay. The lymphocyte subsets were determined by flow cytometry in spleen cell suspension. The mediators of inflammation, TNF-α, IL-6, IL-1β and nitric oxide (NO), were measured in culture supernatant of LPS-primed macrophages isolated from borax treated mice. TNF and ILs were measured by ELISA. NO was determined by Griess test. The expression of inducible nitric oxide synthase (iNOS) in macrophages was studied by confocal microscopy. Results showed a significant increase in T and B cell populations, as indicated by an increase in CD4 and CD19, but not CD8, cells. Boron further stimulated the secretion of TNF-α, IL-6, IL-1β, NO and the expression of iNOS by the LPS-primed macrophages. The effect was dose dependent and most significant at a dose level of 4.6 mg/kg b. wt. Taken together, the study concludes that boron at physiological concentration induces lymphocyte proliferation and increases the synthesis and secretion of pro-inflammatory mediators by the LPS-primed macrophages, more specifically the M1 macrophages, possibly acting through Toll-like receptor. The study implicates boron as a regulator of the immune and inflammatory reactions and macrophage polarization, thus playing an important role in augmenting host defense against infection, with possible role in cancer and other diseases.

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Section: Discussionmentioning

confidence: 99%

Boron Induces Lymphocyte Proliferation and Modulates the Priming Effects of Lipopolysaccharide on Macrophages

Routray

Ali

2016

PLoS ONE

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“…Protein protective property of a substance was determined according to Ali et al [37] method. Hemoglobin solution, O. sanctum extract and mixture (hemoglobin, H 2 O 2 and O. sanctum) were prepared followed by spectral analysis to determine the normal hemoglobin peaks.…”

Section: Spectral Analysismentioning

confidence: 99%

Free Radicals Scavenging and Protein Protective Property of Ocimum sanctum (L)

Keshari

Srivastava

Verma

et al. 2016

BJPR

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Ocimum sanctum (L) is a well-known herbal plant used for the treatment of bronchitis, malaria, diarrhea, dysentery, skin disease, arthritis, eye diseases and insect bites. It is proposed to have anticancer, antidiabetic, antifungal, antimicrobial, cardio protective, analgesic and antispasmodic action. In our present study, we have tried to explore its antioxidant, free radical scavenging and its ability to prevent damage to protein from free radicals. The antioxidant and free radical scavenging activity of Ocimum sanctum leaves was determined using ferric thiocyanate, thiobarbituric Acid, 2, 2-diphenyl-1-picrylhydrazyl, hydrogen peroxide, hydroxyl radical and superoxide radical scavenging methods. Further, its protein protective property was evaluated using UV-VIS spectrophotometer methods. The results of ferric thiocyanate and 2, 2-diphenyl-1-picrylhydrazyl methods were determined that O. sanctum have strong antioxidant activity (92.12%) and free radicals scavenging property (50.74%) respectively. Further, hydrogen peroxide, hydroxyl radical and superoxide radical scavenging methods determined that extracts (100 µg/ml) have hydrogen peroxide (20.12%), hydroxyl radicals (12.68%) and superoxide radicals (21.68%) scavenging

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“…Proteins that would be indistinguishable by either charge or hydrodynamic radius can be distinguished. (B) Comparison of data with literature monomer hydrodynamic radius. − While most proteins match their monomeric size, myoglobin is likely aggregating under the current conditions.…”

Section: Resultsmentioning

confidence: 99%

“…(B) Comparison of data with literature monomer hydrodynamic radius. 49 − 52 While most proteins match their monomeric size, myoglobin is likely aggregating under the current conditions.…”

Section: Resultsmentioning

confidence: 99%

Microscale Diffusiophoresis of Proteins

et al. 2022

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Living systems are characterized by their spatially highly inhomogeneous nature which is susceptible to modify fundamentally the behavior of biomolecular species, including the proteins that underpin biological functionality in cells. Spatial gradients in chemical potential are known to lead to strong transport effects for colloidal particles, but their effect on molecular scale species such as proteins has remained largely unexplored. Here, we improve on existing diffusiophoresis microfluidic technique to measure protein diffusiophoresis in real space. The measurement of proteins is made possible by two ameliorations. First, a label-free microscope is used to suppress label interference. Second, improvements in numerical methods are developed to meet the particular challenges posed by small molecules. We demonstrate that individual proteins can undergo strong diffusiophoretic motion in salt gradients in a manner which is sufficient to overcome diffusion and which leads to dramatic changes in their spatial organization on the scale of a cell. Moreover, we demonstrate that this phenomenon can be used to control the motion of proteins in microfluidic devices. These results open up a path towards a physical understanding of the role of gradients in living systems in the spatial organization of macromolecules and highlight novel routes towards protein sorting applications on device.

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Boron stabilizes peroxide mediated changes in the structure of heme proteins

Cited by 17 publications

References 25 publications

Boron Induces Lymphocyte Proliferation and Modulates the Priming Effects of Lipopolysaccharide on Macrophages

Boron Induces Lymphocyte Proliferation and Modulates the Priming Effects of Lipopolysaccharide on Macrophages

Free Radicals Scavenging and Protein Protective Property of Ocimum sanctum (L)

Microscale Diffusiophoresis of Proteins

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