Botanical origin of<i>Apis</i>pollen loads using colour, weight and pollen morphology data

Barth, Ortrud Monika; Munhoz, Márcia; Luz, Cynthia Fernandes Pinto da

doi:10.1556/aalim.2008.0026

Cited by 26 publications

(19 citation statements)

References 12 publications

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“…Diferentes métodos de investigação palinológica foram aplicados objetivando avaliar a fonte de recurso fl oral e sua utilização como alimento por esses himenópteros (Engel & Dingemans-Bakels 1980, Biesmeijer & Sommeijer 1992, Carvalho et al 2001, Eltz et al 2001, Leal et al 2001Barth 2004, Ramalho 2004, Alves et al 2006, Gutiérrez & García 2007Barth et al 2009). …”

Section: Nota Científi Ca / Scientifi C Noteunclassified

Padrão polínico utilizado por Tetragonisca angustula Latreille (Apidae: Meliponina)

et al. 2011

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ABSTRACT(Pollen used by Tetragonisca angustula Latreille (Apidae: Meliponina)). Th e objective of this study was to quantify and evaluate the pollen load, which was classifi ed by color, found on Tetragonisca angustula Latreille (Apidae: Meliponina). Eighteen pollen types, belonging to 16 plant families, were found on T. angustula. Th e majority of the pollen, from diff erent species, was yellow, indicating that T. angustula prefers this color. Th is was followed by brown pollen and then pumpkin colored pollen. Th e pollen grains that were light colored were the most common. Th e more frequent pollen types were Meliaceae, Myrtaceae (Eucalyptus spp.), Piper mollicomum (Piperaceae), Schizolobium parahyba (Caesalpiniaceae) and Tibouchina granulosa (Melastomataceae).

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Section: Nota Científi Ca / Scientifi C Noteunclassified

Padrão polínico utilizado por Tetragonisca angustula Latreille (Apidae: Meliponina)

et al. 2011

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“…In sequence, it was homogenized and 2 g (wet weight) of it were macerated and extracted with ethanol. The preparation of the pollen pellets followed the standard European methodology (Maurizio and Louveaux 1965) without the use of acetolysis and with some modifications as suggested by Barth et al (2009).…”

Section: Study Areamentioning

confidence: 99%

“…As melissopalynological analysis is rarely made on pollen pellets that are sold on the brazilian market, generally the floral origin that is written on the label is erroneous in which it indicates a vulgar name of a plant, which pollen is not contained in the product (Barth et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Comparative pollen preferences by africanized honeybees Apis mellifera L. of two colonies in Pará de Minas, Minas Gerais, Brazil

Luz

Junior

Fonseca

et al. 2010

An. Acad. Bras. Ciênc.

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The aim of this study was to investigate the polliniferous floral sources used by Apis mellifera (L.) (africanized) in an apiary situated in Pará de Minas, Minas Gerais state, and evaluate the pollen prefences among the beehives. Two beehives of Langstroth type with frontal pollen trap collectors were used. The harvest was made from September 2007 to March 2008, with three samples of pollen pellets colected per month per beehive. The subsamples of 2 grams each were prepared according to the European standard melissopalynological method. A total of 56 pollen types were observed, identifying 43 genus and 32 families. The families that showed the major richness of pollen types were: Mimosaceae (8), Asteraceae (6), Fabaceae (3), Arecaceae (3), Euphorbiaceae (3), Rubiaceae (3), Caesalpiniaceae (2), Moraceae (2) and Myrtaceae (2). The most frequent pollen types (> 45%) were Mimosa scabrella, Myrcia and Sorocea. The results demonstrated a similarity regarding the preferences of floral sources during the major part of the time. There was a distinct utilization of floral sources among the pollen types of minor frequency. In spite of the strong antropic influence, the region showed a great polliniferous variety, which was an indicative of the potential for monofloral as well as heterofloral pollen production.

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“…In this case, single bees are captured and both of their pollen loads were analyzed (Carvalho and Marchini 1999, Marques-Souza et al 2002, Noor et al 2009). Secondly, when pollen traps are used, several pollen loads perform one sample that, for analysis, has to be well mixed; a group of pollen loads, in general selected by its weight or color (Almeida-Muradian 2005, Barth et al 2009, Modro et al 2009a, is used for the recognition of the botanical species composing this pollen sample. The main issue to address is the significance or not of the choice by color.…”

Section: Introductionmentioning

confidence: 99%

“…Commonly, a fragment of a pollen load of each color is analyzed; no sample centrifugation is used. In another way (denoted technique 1), ten pollen loads (Barth et al 2009) or 25 ones of each color batch (Barth, personal information and in the present paper) are mixed using ethanol, and a little amount is analyzed after centrifugation. As pollen grains have different weight, a gradient is obtained, from which pollen grains are removed using a piece of glycerin jelly.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the botanical origin of commercial dry bee pollen load batches using pollen analysis: a proposal for technical standardization

Barth

Freitas

Oliveira

et al. 2010

An. Acad. Bras. Ciênc.

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High quality of bee pollen for commercial purpose is required. In order to attend the consumer with the best identification of the botanical and floral origin of the product, 25 bee pollen batches were investigated using two techniques of pollen grain preparation. The first started to identify pollen loads of different colors in two grams of each well mixed batch, and the second to identify pollen grains in a pool made of all the pollen loads comprised in two grams. The best result was obtained by this last technique, when a pollen grain suspension was dropped on a microscope slide and circa 500 pollen grains were counted per sample. This analysis resulted in the recognition of monofloral and bifloral pollen batches, while the use of the first technique resulted in all samples receiving a heterofloral diagnosis.

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Botanical origin ofApispollen loads using colour, weight and pollen morphology data

Cited by 26 publications

References 12 publications

Padrão polínico utilizado por Tetragonisca angustula Latreille (Apidae: Meliponina)

Padrão polínico utilizado por Tetragonisca angustula Latreille (Apidae: Meliponina)

Comparative pollen preferences by africanized honeybees Apis mellifera L. of two colonies in Pará de Minas, Minas Gerais, Brazil

Evaluation of the botanical origin of commercial dry bee pollen load batches using pollen analysis: a proposal for technical standardization

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