BOVIGAMTM: an in vitro cellular diagnostic test for bovine tuberculosis

Wood, P.R.; Jones, Stephen L.

doi:10.1054/tube.2000.0272

Cited by 172 publications

(144 citation statements)

References 125 publications

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“…As this is an in vitro test that has the advantage of not interfering with the immune status of the animal and may be repeated in the same animal is the need to respect the period of desensitization. This assay showed the increase in the sensitivity and the possibility of more rapid repeat testing, no need for a second visit to the farm and more objective test procedures and interpretation in comparison to the TST (Faye et al, 2011;Neill et al, 1994;Schiller et al, 2010;Wood and Jones, 2001).…”

Section: Gamma Interferon Assays (Bovigam)mentioning

confidence: 99%

Diagnosis of bovine tuberculosis: review of main techniques

2015

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Bovine tuberculosis (BTB) remains an important economic and zoonotic problem in Latin America. Traditionally, the fight against BTB is initiated by the implementation of routine diagnostic tests for certification of free properties. The diagnosis of BTB can be made by direct and indirect methods, in which we can mention clinical, post mortem, histopathological, immunological, bacteriological and molecular methods. The renewal of scientific interest in tuberculosis in recent year has led to develop and improve methods of diagnosis, prevention, control and eradication of BTB. The aim of this review is to present and discuss different diagnosis methods of BTB.Keywords: tuberculosis bovine, diagnosis, Mycobacterium bovis, new techniques. Diagnóstico de tuberculose bovina: revisão das principais técnicas ResumoA tuberculose bovina (BTB) continua sendo um importante problema econômico na América Latina, com potenciais consequências zoonóticas. Tradicionalmente, a luta contra a tuberculose bovina tem sido iniciada pela execução de testes de diagnóstico de rotina para a certificação de propriedades livres da doença. O diagnóstico de BTB pode ser feito através de métodos diretos e indiretos, nos quais podemos citar os métodos clínicos, post mortem, histopatológicos, imunológicos, bacteriológicos e moleculares. A renovação do interesse científico em tuberculose nos últimos anos tem levado à necessidade de desenvolver e melhorar os métodos de diagnóstico, prevenção, controle e erradicação da BTB. O objetivo deste artigo é analisar e discutir sobre os diferentes métodos de diagnóstico de BTB.Palavras-chave: tuberculose bovina, diagnóstico, Mycobacterium bovis, novas técnicas.

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Section: Gamma Interferon Assays (Bovigam)mentioning

confidence: 99%

Diagnosis of bovine tuberculosis: review of main techniques

2015

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“…Wood & Jones (2001) in a comprehensive review also reported that IFN could identify TB positive animals at an earlier time point compared to the CITT, and in some cases positively reacting animals were identified at 14 days post infection (Buddle et al 1995). However, due to its relatively high cost, this assay has been recommended as an auxiliary test (Marassi et al 2009b) to compliment the tuberculin test and mostly in cattle where there is a high probability of infection.…”

Section: Discussionmentioning

confidence: 99%

“…The Gamma-Interferon assay (IFN) is also based on the cell-immune response and measures IFN released into whole-blood culture (Wood et al 1990) in vitro, in response to specific antigen stimulation. This assay has been evaluated as a primary diagnostic method in many countries (Wood 2001), including Brazil, and has some important advantages (Lilenbaum, 1999). Since lymphocyte stimulation is done in vitro, and consequently does not alter the immune status of the animal, it is not necessary to wait 60-90 days to repeat the test when the initial test is inconclusive, a distinct limitation of the skin tests.…”

Section: Introductionmentioning

confidence: 99%

A multidisciplinary approach to diagnose naturally occurring bovine tuberculosis in Brazil

et al. 2013

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“…Apart from the tuberculin test, the interferon-gamma release assay has also been approved by the European Union for the ante-mortem diagnostics of bovine tuberculosis (6). Its aim is to detect interferon-gamma secreted in vitro by the leukocytes of an infected animal (3,4,16). It is performed with the use of whole blood samples collected into tubes containing lithium heparin or any other anticoagulant, which are then incubated with tuberculin in an incubator (37°C; ± 2°C).…”

Section: Methodsmentioning

confidence: 99%

Ante-mortem and post-mortem tuberculosis diagnostics in three European Bison from the enclosure in Bukowiec in the Bieszczady National Park in Poland

Anusz¹,

Orłowska²,

Krajewska‐Wędzina³

et al. 2017

Medycyna Weterynaryjna

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SummaryThe ante-mortem diagnostics of tuberculosis in wildlife constitutes a major problem due to the difficulties in collecting samples for examination. Therefore, tuberculosis in free-living animals is usually diagnosed post mortem. Ante-mortem examinations for bovine tuberculosis (BTB) were conducted in three European bison living in an acclimatisation enclosure in Bukowiec in the Bieszczady National Park in Poland. The reason for performing these examinations was the documented contact between the aforementioned animals and tuberculous European bison from a free-living herd present in the area. The three European bison were pharmacologically immobilised. Next, their blood samples were collected for immunological studies (interferon-gamma release assays), complete blood count and blood biochemistry tests. Moreover, bronchoalveolar lavage (BAL) fluid from the lower respiratory tract, two nasal cavity swabs and one throat swab were taken from each animal. Tuberculin testing was also performed. The findings of all these examinations indicated the possible infection of the European bison with the tubercle bacillus. Ultimately, the decision was taken to euthanize the animals.

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BOVIGAMTM: an in vitro cellular diagnostic test for bovine tuberculosis

Cited by 172 publications

References 125 publications

Diagnosis of bovine tuberculosis: review of main techniques

Diagnosis of bovine tuberculosis: review of main techniques

A multidisciplinary approach to diagnose naturally occurring bovine tuberculosis in Brazil

Ante-mortem and post-mortem tuberculosis diagnostics in three European Bison from the enclosure in Bukowiec in the Bieszczady National Park in Poland

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