Bovine herpesvirus type 5 in semen samples from bulls in Iran

Sharifzadeh, Ali; Namazi, Mohammad Javad; Mokhtari-Farsani, Abbas; Doosti, Abbas

doi:10.1007/s00705-014-2272-3

Cited by 8 publications

(8 citation statements)

References 17 publications

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“…Khamesipour et al ( 2013 ) detected the virus DNA in nine out 45 frozen semen samples from seropositive bulls. Other authors described similar results (Dus Santos et al 2006 ; Sharifzadeh et al 2011 ; Asadpour and Jafari 2012 ).…”

Section: Introductionsupporting

confidence: 66%

Seroprevalence and detection of bovine leukosis virus in semen from breeding bulls in Costa Rica

Chacón,

Leiva,

Romero-Zuñiga

et al. 2023

Trop Anim Health Prod

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Bovine leukemia virus (BLV) causes enzootic bovine leukosis, a persistent infection and the most important neoplastic disease in cattle. It is spread primarily by transferring infected lymphocytes through blood from carriers to healthy animals. The present study is aimed at determining the seropositivity of BLV in breeding bulls from Costa Rica and at detecting for the first time in the country BLV DNA in bull semen. Between May 2011 and August 2018, 379 blood and 133 semen samples were collected from bulls distributed in 118 farms. The serum was analyzed by an enzymatic immunoassay and the semen by polymerase chain reaction and sequencing. BLV seropositivity was 43.5% (165/379), while 64.4% (76/118) of the farms had positive reactors. Holstein (75.7%) and Jersey (73.0%) breeds showed the highest seropositivity. In addition, Bos taurus bulls (68.1%), older than seven years (50.0%), and those belonging to dairy farms (75.5%) had higher seropositivity compared to Bos indicus (17.7%), younger than seven years (42.2%), and those from beef farms (15.5%), respectively. Moreover, Bos taurus bulls had a higher risk of being seropositive than Bos indicus (OR = 3.4; 95% CI: 1.7–6.8). BLV DNA was found in one semen sample (2.5%; 1/40) from a seropositive bull. The importance of serum and molecular BLV screening in semen samples and the potential role of some risk factors associated with the disease, such as the bull’s age, genotype, and type of livestock productive system, is argued in the present report.

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Section: Introductionsupporting

confidence: 66%

Seroprevalence and detection of bovine leukosis virus in semen from breeding bulls in Costa Rica

Chacón,

Leiva,

Romero-Zuñiga

et al. 2023

Trop Anim Health Prod

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“…BoHV5 is endemic in South American countries-Argentina [9], Uruguay [10] and Brazil [11]. Only a few cases of the disease have been reported in Australia [12], Hungary [13], Iran [14], Canada [15] and United States [16]. BoHV5 has not been reported in India.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India

et al. 2020

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Bovine herpesvirus 1 (BoHV1) and 5 (BoHV5) are genetically and antigenically related alphaherpesviruses. Infection with one virus induces protective immunity against the other. However, disease associated with BoHV1 and BoHV5 varies significantly; whereas BoHV1 infection is usually associated with rhinotracheitis and abortion, BoHV5 causes encephalitis in cattle. BoHV5 outbreaks are sporadic and mainly restricted to the South American countries. We report BoHV5 infection for the first time from aborted cattle in India. Based on the characteristic cytopathic effects in MDBK cells, amplification of the viral genome by PCR, differential PCR for BoHV1/BoHV5, nucleotide sequencing and restriction endonuclease patterns, identity of the virus was confirmed as BoHV5 subtype A. Serum samples from the aborted cattle strongly neutralized both BoHV1 and BoHV5 suggesting an active viral infection in the herd. Upon UL27, UL44 and UL54 gene-based sequence and phylogenetic analysis, the isolated virus clustered with BoHV5 strains and showed highest similarity with the Brazilian BoHV5 strains.

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“…BHV5 is endemic in South American countries-Argentina [9], Uruguay [10] and Brazil [11]. Only a few cases of the disease have been reported in Australia [12], Hungary [13], Iran [14], Canada [15] and United States [16]. BHV5 has not been reported in India.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of bovine herpes virus 5 (BHV5) from cattle in India

Kumar

Chander

Riyesh

et al. 2020

Preprint

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15Bovine herpesvirus 1 (BHV1) and 5 (BHV5) are genetically and antigenically related 16 alphaherpesviruses. Infection with one virus induces protective immunity against the other. 17However, disease associated with BHV1 and BHV5 varies significantly; whereas BHV1 18 infection is usually associated with rhinotracheitis and abortion, BHV5 causes encephalitis in 19 cattle. BHV5 outbreaks are sporadic and mainly restricted to the South American countries. 20 We report BHV5 infection for the first time from aborted cattle in India. Based on the 21 characteristic cytopathic effects in MDBK cells, amplification of the viral genome in PCR, 22 differential PCR for BHV1/BHV5, nucleotide sequencing and restriction endonuclease 23 patterns, identity of the virus was confirmed as BHV5 subtype A. Serum samples from the 24 aborted cattle strongly neutralized both BHV1 and BHV5 suggesting an active viral infection 25 in the herd. Upon UL27, UL44 and UL54 gene-based sequence and phylogenetic analysis, the 26 isolated virus clustered with BHV5 strains and showed highest similarity with the Brazilian 27 BHV5 strains. 28 Author Summary 29BHV5 causes fatal meningoencephalitis that may result in a mortality rate of up to 100% in 30 cattle. BHV5 is rarely associated with abortion and its distribution is restricted to South 31 American countries. Only a few cases of this disease have been reported from other countries. 32For the first time, we provide a strong evidence of BHV5 infection from aborted cattle in 33 India. The finding may necessitate inclusion of BHV5 test protocol in testing of semen for 34 sexually transmitted diseases. Also, the isolated virus would be useful for developing 35 diagnostic, prophylactic and therapeutic agents to combat BHV5 disease in the country. 3 37 Introduction 38 Bovine herpesvirus types 1 (BHV1) and 5 (BHV5) belong to the family Herpesviridae, 39 subfamily Alphaherpesvirinae and genus varicellovirus [1]. They are genetically and 40 antigenically closely related and share ~85% nucleotide identity. BHV1 is prevalent globally 41 and causes infectious bovine rhinotracheitis (IBR) in cattle which is characterized by 42 conjunctivitis, rhinotracheitis, balanopostitis, infectious pustular vulvovaginitis, enteritis, 43 abortion and sometimes encephalitis. BHV1 infection results in considerable economic losses 44 due to decreased milk production, weight loss and abortions. 45 BHV5 usually infects young calves and mortality can causes up to 100% [2]. BHV5 46 mainly causes fatal meningoencephalitis in cattle and establishes latency in trigeminal 47 ganglion. The virus is excreted in nasal, ocular and genital secretions upon reactivation 48 (under stress). The clinical signs in affected cattle include depression, anorexia, weakness 49 followed by neurological signs, such as incoordination, muscular tremor, blindness, circling, 50 recumbency, head pressing, convulsions, paddling and death [3]. Occasionally, BHV5 has 51 been shown to be associated with the reproductive disorders [4]. 52 Herpesvirus ass...

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Bovine herpesvirus type 5 in semen samples from bulls in Iran

Cited by 8 publications

References 17 publications

Seroprevalence and detection of bovine leukosis virus in semen from breeding bulls in Costa Rica

Seroprevalence and detection of bovine leukosis virus in semen from breeding bulls in Costa Rica

Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India

Isolation and characterization of bovine herpes virus 5 (BHV5) from cattle in India

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