Bovine salivary progesterone: Application to the assessment of ovarian function and early pregnancy diagnosis

Kanchev, L.N.; Marinova, C.; Stankov, Bojidar

doi:10.1016/0378-4320(88)90040-1

Cited by 6 publications

(12 citation statements)

References 22 publications

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“…Saliva P4 concentration in cattle in estrus in this study was higher than in previous reports in Friesian/Jersey cows [16]. It has been demonstrated that P4 levels during the follicular phase fluctuates from 0.025 to 0.08ng/ml [15]. Although P4 levels are low when cows/heifers are in estrus, a low concentration of P4 does not necessary mean the animal is in estrus, as anestrous cattle with small ovaries without palpable follicles or CL, also have low levels of P4.…”

Section: P4 Detection In Non-pregnant Cycling and Anestrous Cattlecontrasting

confidence: 54%

“…Samples were stored at -80 o C until assayed. Collection procedures were based on previous reports [15,16].…”

Section: Sample Collectionmentioning

confidence: 99%

“…Determination of P4 in saliva using ELISA has also been used to determine the reproductive status in farm animals [15][16][17] as well as in women [18]. However, determination of P4 concentration alone is not sufficient to predict ovulation because there is a large variation in the timing of P4 decrease relative to ovulation [19].…”

Section: Introductionmentioning

confidence: 99%

“…Changes in total P4 circulation in blood (protein -bound plus free, non-protein bound fractions) have been widely used for accurate monitoring of reproductive status in cows [15,16,[38][39][40]. Over 80% of circulating P4 is bound to plasma proteins [41,42] and only the free fraction is biologically active [43,44].…”

Section: Introductionmentioning

confidence: 99%

“…Sex steroids in saliva can be used as an index of their unbound plasma concentration [43,45,46]. Hence, the quantitative correlation between the two fractions could be used to determine P4 concentration in matched blood and saliva samples [15,47,48].…”

Section: Introductionmentioning

confidence: 99%

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Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

Mekonnin¹,

Howie²,

Riley³

et al. 2017

Anim Husb Dairy Vet Sci

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Effective breeding management is a crucial tool to enhance the reproductive and productive performance of dairy cattle. To achieve this, regular monitoring of their reproductive status is important. The aims of this study were to monitor the reproductive status of crossbred dairy cattle based on progesterone (P4) concentration in serum, whole milk, saliva and urine, and estradiol in serum using quantitative laboratory ELISA tests, and to document hormonal profiles at different reproductive stages. Three hundred and thirty-six (336) crossbred (Holstein Friesian x Zebu) dairy cattle (232 cows and 104 heifers) from 47 dairy farms owned by smallholder or organized commercial dairy farmers were included in the study. Matched blood, milk and saliva samples were collected twice at 11-day intervals at afternoon milking, while urine was collected just once. Serum was separated within 48 hr of blood collection. All samples were stored at -80 o C until assayed. In both cows and heifers, P4 was higher during pregnancy than in other reproductive conditions. Estradiol was higher during estrus than at other reproductive stages. Estradiol levels were significantly higher (p<0.05) in estrus cows compared with heifers. The mean level of P4 in serum strongly correlated with the value in milk (r = 0.6368, P< 0.0001), however there was no correlation in P4 concentration between serum and saliva or between P4 in serum and in urine. Hence, determination of P4 in serum and milk using an ELISA test is a reliable and precise method to monitor reproductive status in crossbred dairy cattle.

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Section: P4 Detection In Non-pregnant Cycling and Anestrous Cattlecontrasting

confidence: 54%

“…Samples were stored at -80 o C until assayed. Collection procedures were based on previous reports [15,16].…”

Section: Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

Mekonnin¹,

Howie²,

Riley³

et al. 2017

Anim Husb Dairy Vet Sci

View full text Add to dashboard Cite

show abstract

Reproductive hormone concentrations in faeces during the oestrous cycle and pregnancy in cattle (Bos taurus) and muskoxen (Ovibos moschatus)

Desaulniers¹,

Goff²,

Betteridge³

et al. 1989

Can. J. Zool.

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Difficulties associated with blood sampling of wild animals are a deterrent to reproductive research but might be circumvented by the development of noninvasive techniques. Samples of faeces were collected from 4 cyclic and 19 pregnant domestic cows and from 6 female muskoxen during anoestrus, the oestrous cycle, and pregnancy. In cows, the faecal progesterone profile corresponded well with plasma values in cyclic and pregnant animals. Increased concentrations of 15-keto-13,14-dihydroprostaglandin F2α could be detected in bovine faeces during luteolysis using data from a group of animals. A large increase in total oestrogen occurred in both faeces and plasma after the 12th week of pregnancy, confirming that faecal oestrogen levels can be used for pregnancy diagnosis. In the muskox, faecal concentations of total oestrogen and progesterone were correlated with reproductive status, and animals that were anoestrous, oestrous, pregnant, or in the luteal phase of the cycle could be recognized.

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Monitoring Reproductive Status by Fecal Progesterone Analysis in Ruminants.

Hirata

Mori²

1995

J. Vet. Med. Sci.

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Bovine salivary progesterone: Application to the assessment of ovarian function and early pregnancy diagnosis

Cited by 6 publications

References 22 publications

Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

Serum, milk, saliva and urine progesterone and estradiol profiles in crossbred (Zebu x Holstein Friesian) dairy cattle

Reproductive hormone concentrations in faeces during the oestrous cycle and pregnancy in cattle (Bos taurus) and muskoxen (Ovibos moschatus)

Monitoring Reproductive Status by Fecal Progesterone Analysis in Ruminants.

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